Effect of Red Dragon Fruit Extract Ingestion on Mitochondrial Cytochrome c Levels in Rat Soleus Muscle Following Moderate Exercise

Ade Indra Mukti, Gusbakti Gusbakti, Putri Handayani, Dewi Sartika, Alvin Abrar Harahap, Sulaiman Delrizal

Abstract


Background: Exercise-induced free radicals harm cellular mitochondria and trigger apoptosis via cytochrome-c release. Red dragon fruit (RDF) extract is known for its potent antioxidant properties. This study aimed to investigate whether RDF extract could counteract oxidative stress and apoptosis in rat soleus muscle post-exercise.

Methods: This experimental study employed a randomized posttest-only control group design, conducted at the Animal House Unit of the Biology Laboratory, Universitas Sumatera Utara. Using 25 male rats, approximately 3 months old and weighing 200 g, selected by simple random sampling. Subjects were divided into five groups: sedentary control (K1), exercise-only (K2), and three test groups receiving RDF extract (75, 150, and 300 mg/kg) alongside moderate-intensity swimming for 31 days. Measured variables included malondialdehyde (MDA), cytochrome-c, and muscle histology. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests (p<0.05).

Results: RDF-treated rats showed significantly lower levels of MDA and cytochrome-c compared to the exercise-only group. The Kruskal-Wallis test revealed significant differences among groups for both MDA (p=0.002) and cytochrome-c (p=0.003). Post-hoc analysis showed that the exercise-only group (K2) exhibited the highest cytochrome-c levels (mean rank 16.50), whereas the highest RDF dose (300 mg/kg) significantly reduced it (mean rank 6.75, p=0.008 vs. K2). Histopathological analysis confirmed less muscle tissue damage and reduced inflammatory cell infiltration in extract-treated groups.

Conclusions: RDF extract suppresses exercise-induced oxidative stress, inhibits apoptotic signaling, and protects muscle tissue, demonstrating its potential as a post-exercise recovery supplement. 


Keywords


Cytochrome-c, oxidative stress, physical exercise, red dragon fruit extract, soleus muscle

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References


  1. Furrer R, Handschin C. Molecular aspects of the exercise response and training adaptation in skeletal muscle. Free Radic Biol Med. 2024;223:53–68. doi:10.1016/j.freeradbiomed.2024.07.026
  2. Furrer R, Hawley JA, Handschin C. The molecular athlete: exercise physiology from mechanisms to medals. Physiol Rev. 2023;103(3):1693–787. doi:10.1152/physrev.00017.2022
  3. Protasoni M, Zeviani M. Mitochondrial Structure and Bioenergetics in Normal and Disease Conditions. Int J Mol Sci. 2021;22(2):586. doi:10.3390/ijms22020586
  4. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. 2017;2017:8416763. doi:10.1155/2017/8416763
  5. Bhatti JS, Bhatti GK, Reddy PH. Mitochondrial dysfunction and oxidative stress in metabolic disorders - A step towards mitochondria based therapeutic strategies. Biochim Biophys Acta Mol Basis Dis. 2017;1863(5):1066–77. doi:10.1016/j.bbadis.2016.11.010
  6. Redza-Dutordoir M, Averill-Bates DA. Activation of apoptosis signalling pathways by reactive oxygen species. Biochim Biophys Acta. 2016;1863(12):2977–92. doi:10.1016/j.bbamcr.2016.09.012
  7. Hussar P. Apoptosis regulators Bcl-2 and caspase-3. Encyclopedia. 2022;2(4):1624–36. doi:10.3390/encyclopedia2040111
  8. Mustafa M, Ahmad R, Tantry IQ, Ahmad W, Siddiqui S, Alam M, et al. Apoptosis: a comprehensive overview of signaling pathways, morphological changes, and physiological significance and therapeutic implications. Cells. 2024;13(22):1838. doi:10.3390/cells13221838
  9. Zhang M, Zheng J, Nussinov R, Ma B. Release of cytochrome c from bax pores at the mitochondrial membrane. Sci Rep. 2017;7(1):2635. doi:10.1038/s41598-017-02825-7
  10. Anwar H, Hussain G, Mustafa I. Antioxidants from natural sources. In: Shalaby E, editor. Antioxidants in Foods and Its Applications. London: IntechOpen; 2018. doi:10.5772/intechopen.75961
  11. Monserrat-Mesquida M, Quetglas-Llabrés M, Bouzas C, García S, Mateos D, Gómez C, et al. Effects of 2-year nutritional and lifestyle intervention on oxidative and inflammatory statuses in individuals of 55 years of age and over at high cardiovascular risk. Antioxidants (Basel). 2022;11(7):1326. doi:10.3390/antiox11071326
  12. Marrazzo P, O’Leary C. Repositioning natural antioxidants for therapeutic applications in tissue engineering. Bioengineering. 2020;7(3):104. doi:10.3390/bioengineering7030104
  13. Chen SY, Xu CY, Mazhar MS, Naiker M. Nutritional value and therapeutic benefits of dragon fruit: a comprehensive review with implications for establishing australian industry standards. Molecules. 2024;29(23):5676. doi:10.3390/molecules29235676
  14. Yuna P, Chiuman L, Ginting CN. Anti-inflammatory effect of red dragon fruit (hylocereus polyrhizus) peel on male white rat. JFIKI. 2023;10(1):22–9. doi: 10.20473/jfiki.v10i12023.22-29
  15. Mulyati AH, Nurmayani R, Widiastuti D, Warnasih S, Sinaga SE. Antioxidant potential and active compound identification of hylocereus costaricensis and hylocereus undatus peel extracts using LC-MS/MS. Chim Nat Acta. 2025;13(2):175–84. doi: 10.24198/cna.v13.n2.61186
  16. Gusti AMT, Qusti SY, Alshammari EM, Toraih EA, Fawzy MS. Antioxidants-related superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, and nitric oxide synthase gene variants analysis in an obese population: a preliminary case-control study. Antioxidants (Basel). 2021;10(4):595. doi:10.3390/antiox10040595
  17. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase, catalase, and glutathione peroxidase: their fundamental role in the entire antioxidant defence grid. Alexandria J Med. 2018;54(4):287–93. doi:10.1016/j.ajme.2017.09.001
  18. Luu T, Le T, Huynh N, Quintela-Alonso P. Dragon fruit: a review of health benefits and nutrients and its sustainable development under climate changes in Vietnam. Czech J Food Sci. 2021;39(2):71–94. doi:10.17221/139/2020-CJFS
  19. Rusip G, Ilyas S, Lister INE, Ginting CN, Mukti I. The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (Rattus norvegicus). F1000Res. 2021;10:1061. doi:10.12688/f1000research.54254.3
  20. Rizo-Roca D, Ríos-Kristjánsson JG, Núñez-Espinosa C, Ascensão A, Magalhães J, Torrella JR, et al. A semiquantitative scoring tool to evaluate eccentric exercise-induced muscle damage in trained rats. Eur J Histochem. 2015;59(4):2544. doi:10.4081/ejh.2015.2544.
  21. Nirmal S, Olatunde OO, Medhe S, Vitti S, Khemtong C, Nirmal NP. Betalains alleviate exercise-induced oxidative stress, inflammation, and fatigue and improve sports performance: an update on recent advancement. Curr Nutr Rep. 2023;12(4):778–87. doi:10.1007/s13668-023-00500-0
  22. Ke Y, Mitacek RM, Abraham A, Mafi GG, VanOverbeke DL, DeSilva U, et al. Effects of muscle-specific oxidative stress on cytochrome c release and oxidation–reduction potential properties. J Agric Food Chem. 017;65(35):7749–55. doi:10.1021/acs.jafc.7b01735
  23. Feng W, Wang Y, Gu X, Yu D, Liu Z. Exercise as a modulator of systemic inflammation and oxidative stress biomarkers across clinical and healthy populations: an umbrella meta-analysis. BMC Sports Sci Med Rehabil. 2025 Nov 28;17(1):360. doi:10.1186/s13102-025-01327-8
  24. Guo Y, Qian H, Xin X, Liu Q. Effects of different exercise modalities on inflammatory markers in the obese and overweight populations: unraveling the mystery of exercise and inflammation. Front Physiol. 2024;15:1405094. doi:10.3389/fphys.2024.1405094
  25. Santoso A, Amalia E, Sari K, Takarini V, Sufiawati I. Histopathological Evaluation of Wound Healing and Anti-Inflammatory Effects of Granola Potato Peel Ethanol Extract in Rat Oral Mucosa. J Exp Pharmacol. 2024;16:377–95. doi:10.2147/JEP.S487373
  26. Gupta A, Kumar P. Assessment of the histological state of the healing wound. Plast Aesthet Res. 2015;2:239–42. doi:10.4103/2347-9264.158862
  27. Grevendonk L, Connell NJ, McCrum C, Fealy CE, Bilet L, Bruls YMH, et al. Impact of aging and exercise on skeletal muscle mitochondrial capacity, energy metabolism, and physical function. Nat Commun. 2021;12(1):4773. doi:10.1038/s41467-021-24956-2
  28. Li J, Wang Z, Li C, Song Y, Wang Y, Bo H, et al. Impact of exercise and aging on mitochondrial homeostasis in skeletal muscle: roles of ROS and epigenetics. Cells. 2022;11(13):2086. doi:10.3390/cells11132086




DOI: https://doi.org/10.15850/amj.v13n2.4596

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