Antibacterial Activity of Mangrove Leaves Extract (Rhizopora Apiculata) Against Salmonella typhi Growth

Nurfadly Nurfadly, Sevani Ayu Harahap, Ance Roslina, Munauwarus Sarirah

Abstract


Mangrove leaves (Rhizopora apiculata) contain antibacterial chemical compounds with antibacterial properties against various pathogens. Salmonella typhi, the causative agent of thyphoid fever, triggers a systemic infectious disease that can lead to complications and deaths if not treated properly.  This study aimed to screen the phytochemical content of R. apiculata leaf extract, evaluate its antibacterial activity against S. typhi, and determine the optimal inhibitory concentration. Leaves were collected from the Sicanang mangrove forest in Belawan, North Sumatra, Indonesia, and extracted using the maceration method with 96% ethanol.  Antibacterial effectiveness was assessed using disc diffusion method by measuring the zone of inhibition after exposure to mangrove leaf extract at concentrations of 40%, 60%, 80%, and 100%, with chloramphenicol as a positive control, to determine the most effective concentration for inhibiting the growth of Salmonella typhi. The results of the study showed that there were differences in inhibition zones in each group. Mangrove leaves extract at a concentration of 100% is the most effective in inhibiting the growth of Salmonella typhi compared to 80%, 60%, and 40% concentrations.

Keywords


Mangrove leaves, rhizopora apiculata, salmonella typhi

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References


  1. Genilar LA, Kurniawaty E, Mokhtar RA, Audah KA. Mangroves and their medicinal benefit: a mini review. Ann RSCB. 2021;25(4):695–709.
  2. Wardina MA, Mustofa S, Malarangeng| AFTA Review Article: Potensi Rhizophora apiculata Sebagai Fitofarmaka. Medula. 2023;13(2): 137–46.
  3. Mutik MS, Sibero MT, Widianingsih W, Subagiyo S, Pribadi R, Haryanti D, et al. Kandungan senyawa bioaktif dan aktivitas biologis ekstrak daun Rhizophora apiculata asal perairan Teluk Awur, Jepara. J Kelaut Trop. 2022;25(3):378–90.
  4. Li Y, Kong D, Fu Y, Sussman MR, Wu H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol Biochem. 2020;148:80–89. doi:10.1016/j.plaphy.2020.01.006
  5. Widiani DY, Aliyah SD, Awwalia EF, Al Husen IM, Komarudin P. Antibacterial test of Rhizophora apiculata extract originated from Mangrove Wonorejo Surabaya against Staphylococcus aureus and Escherichia coli. J Marine Resources Coastal Management. 2023;4(1):6–10.
  6. Acharya S, Jali P, Pradhan M, Pradhan C, Mohapatra PK. Antimicrobial and antioxidant property of a true mangrove rhizophora apiculata Bl. Chem Biodivers. 2023;20(9):e202201144. doi:10.1002/cbdv.202201144
  7. Marchello CS, Hong CY, Crump JA. Global typhoid fever incidence: A systematic review and meta-analysis. Clin Infect Dis. 2019;68(Suppl2):S105–S116. doi:10.1093/cid/ciy1094
  8. Ferrari RG, Rosario DKA, Cunha-Neto A, Mano SB, Figueiredo EES, Conte-Juniora CA. Worldwide epidemiology of Salmonella serovars in animal-based foods: A meta-analysis. Appl Environ Microbiol. 2019;85(14):e00591–19. doi:10.1128/AEM.00591-19
  9. Hancuh M, Walldorf J, Minta AA, et al. Typhoid fever surveillance, incidence estimates, and progress toward typhoid conjugate vaccine introduction—worldwide, 2018-2022. MMWR Morb Mortal Wkly Rep. 2023;72(7):171–6. doi:10.15585/mmwr.mm7207a2
  10. Hancuh M, Walldorf J, Minta AA, Tevi-Benissan C, Christian KA, Nedelec Y. Typhoid fever infection – Antibiotic resistance and vaccination strategies: A narrative review. Travel Medicine and Infectious Disease. 2021;40(101946):2–15
  11. Kuehn R, Stoesser N, Eyre D, Darton TC, Basnyat B, Parry CM. Treatment of enteric fever (typhoid and paratyphoid fever) with cephalosporins. Cochrane Database Syst Rev. 2022;11(11):CD010452. doi: 10.1002/14651858.CD010452.pub3
  12. Terreni M, Taccani M, Pregnolato M. New antibiotics for multidrug-resistant bacterial strains: latest research developments and future perspectives. Molecules. 2021;26(9):2671. doi:10.3390/molecules26092671
  13. Alhaddad ZA, Tanod WA, Wahyudi D. Bioaktivitas antibakteri dari ekstrak daun mangrove avicennia sp. J Kelaut Indones J Mar Sci Technol. 2019;12(1):12–22
  14. Keerthi RR, Baskar K, Mavishna M, Johnson A. Antibacterial and antioxidant activity of mangrove plant (Avicennia Marina) extract against some specific oral pathogens. Int J Health Sci. 2022;6(3):1348–55. doi:10.53730/ijhs.v6nS3.5562
  15. Akasia AI, Nurweda Putra IDN, Giri Putra IN. Skrining fitokimia ekstrak daun mangrove rhizophora mucronata dan rhizophora apiculata yang dikoleksi dari Kawasan Mangrove Desa Tuban, Bali. J Mar Res Technol. 2021;4(1):16–22.
  16. Masuet-Aumatell C, Atouguia J. Typhoid fever infection - Antibiotic resistance and vaccination strategies: A narrative review. Travel Med Infect Dis. 2021;40:101946. doi: 10.1016/j.tmaid.2020.101946
  17. Arifin MH, Yacoeb AM, Abdullah A, Riyadi PH. Drying method comparison of black mangrove leaves (Rhizophora mucronata) for an antioxidant activity assay. Food Res. 2023;7(Suppl3):67–75. doi:10.26656/fr.2017.7(S3).095
  18. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6(2):71–9. doi:10.1016/j.jpha.2015.11.005
  19. Eswaraiah G, Peele AK, Krupanidhi S, Kumar RB, Venkateswaru TC. Studies on phytochemical, antioxidant, antimicrobial analysis and separation of bioactive leads of leaf extract from the selected mangroves. J King Saud Univ Sci. 2020;32(1):842–7. doi:10.1016/j.jksus.2018.11.014
  20. Summer K, Browne J, Hollanders M, Benkendorff K. Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO). Biofilm. 2022;4:100081. doi:10.1016/j.bioflm.2022.100081
  21. Karundeng EDB, Hanizar E, Sari DNR. Potensi Ekstrak Daun Rhizophora mucronata Sebagai Antibakteri pada Staphylococcus aureus. Biosapphire J Biol dan Divers. 2022;1(1):10–18.
  22. Andriani S, Putri A, Khairani IA, et al. Aktivitas antibakteri ekstrak daun mangrove terhadap bakteri Escherichia coli. J Sci Med Lab. 2023;1(1):14–22.
  23. Hafizhah R, Muthmainah N, Biworo A. Literature review: pola kepekaan salmonella typhi terhadap antibiotik. Homeostasis. 2021;4(3):773–84.
  24. Cooper CR. Antimicrobial agent disk susceptibility table. Microbiology laboratory. [Internet] 2019 [cited 2024 September 12]. Available from: http://crcooper01.people.ysu.edu/microlab/ast-chart.pdf.




DOI: https://doi.org/10.15395/mkb.v57.4092

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