Antioxidant and Antiaging Properties of Ethanolic Ripe Sesoot Fruit Extract

Sri Utami, Insan Sosiawan, Dewi Nurul, Endah Purnamasari, Lilian Batubara, Qomariyah Romadhiyani Sachrowardi, Ndaru Andri Damayanti, Aryenti Aryenti, Intan Keumala Dewi, Said Nafik, Betharie Cendera Arrahmani, Hanna Sari Widya Kusuma, Wahyu Widowati, Herry S Utomo

Abstract


Skin aging can be characterized by changes in skin, such as the appearance of wrinkles and loss of skin moisture. Some elements that might lead to cell damages and aging are free radicals through the increase of the activities of hyaluronidase and tyrosinase. Bioactive compounds found in sesoot (Garcinia picrorhiza Miq.) are known for their antioxidant properties, which may reduce excessive amounts of free radicals in the body and act as an antiaging agent by inhibiting hyaluronidase and tyrosinase activities. This study aimed to examine the antioxidant, anti-hyaluronidase, and anti-tyrosinase properties found in Ethanolic Extract Ripe Sesoot (EERS). The ethanol extract of ripe sesoot fruit obtained from LIPI, Bogor Botanical Garden was used for the antioxidant and antiaging assays conducted at PT Aretha Medika Utama in December 2016. The activity of antioxidants was measured as the Ferric Reducing Antioxidant Power (FRAP) of the EERS, while the antiaging assays were performed through the inhibition activity of hyaluronidase and tyrosinase. Results indicated that the EERS has a higher FRAP activity (17.58 μM Fe (II)/μg) than xanthone (2.54 μM Fe (II)/μg) at the highest concentration of sample of 5,000 µg/mL. The anti-hyaluronidase of the EERS exhibited lower activity (IC50 of 619.21±12.15 µg/mL) than xanthone (IC50 of 365.55±25.10 µg/mL) and the tyrosinase inhibitory assay demonstrated a lower activity of EERS (IC50 of 1060.68±12.81 µg/mL) compared to xanthone (IC50 of 218.33±9.73 µg/mL). To conclude, EERS shows antioxidant and antiaging properties.

Keywords


Antioxidant, Garcinia picrorhiza, hyaluronidase, skin aging, tyrosinase

Full Text:

PDF

References


  1. Genovese L, Corbo A, Sibilla S. An insight into the changes in skin texture and properties following dietary intervention with a nutricosmeceutical containing a blend of collagen bioactive peptides and antioxidants. Skin Pharmacol Physiol. 2017;30(3):146–58. doi:10.1159/000464470
  2. Miranda AF, Kenneth WM, Peter E, Howard IM., Characteristics of the aging skin. Adv Wound Care. 2013;2(1):5–10. doi:10.1089/wound.2011.0356
  3. Rizzi V, Gubitosa J, Fini P, Cosma P. Neurocosmetics in skincare-the fascinating world of skin-brain connection: A review to explore ingredients, commercial products for skin aging, and cosmetic regulation. Cosmetics. 2021;8(3):66.
  4. Pintus F, Floris S, Fais A, Era B, Kumar A, Gatto G, et al. Hydroxy-3-Phenylcoumarins as multitarget compounds for skin aging diseases. synthesis, molecular docking and tyrosinase, elastase, collagenase and hyaluronidase inhibition, and sun protection factor. Molecules. 2022;27(20):6914. doi:10.3390/molecules27206914
  5. D’Mello SAN, Finlay GJ, Baguley BC, Askarian-Amiri ME. Signaling pathways in melanogenesis. Int J Mol Sci. 2016;17(7):1144. doi:10.3390/ijms17071144
  6. Krumova K, Cosa G. Chapter 1: Overview of reactive oxygen species. Application in Biosciences and Nanosciences. 2016;1(2016):1–21.
  7. Utami S, Sachrowardi QR, Damayanti NA, Wardhana A, Syarif I, Arrahman BC, et al. Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorhiza Miq.) fruit as antiaging. J Herb Med Pharmacol. 2018;7(2):88–93.
  8. Sukandar ER, Kaennakam S, Raab P, Nöst X, Rassamee K, Bauer R, et al. Cytotoxic and Anti-Inflammatory Activities of Dihydroisocoumarin and Xanthone Derivatives from Garcinia picrorhiza. Molecules. 2021;26(21):6626. doi:10.3390/molecules26216626
  9. Utami S, Laksono SP, Sachrowadi QR, Mustaqimah DN, Endrini S, Damayanti NA, Nafik S, Arrahmani BC, Kusuma HSW, Widowati W. In-vitro studies on antioxidant and antidiabetic potential of Sesoot (Garcinia picrorhiza Miq.) fruit ethanolic extract from Indonesia. Pharmaciana. 2021;11(2):273–82.
  10. Widowati W, Fauziah N, Heddy H, Afni M, Afifah E, Kusuma HSW, et al. Antioxidant and antiaging assays of Oryza sativa extracts, vanilin and coumaric acid. J Nat Remed. 2016;16(3):88–99.
  11. Cane HPCA, Saidi N, Mustanir M, Darusman D, Idroes R, Musman M. Evaluation of antibacterial and antioxidant activities of xanthone isolated from orophea corymbosa leaf. Rasayan J Chem. 2020;13(4):2215–22.
  12. Widowati W, Widyanto RM, Husin W, Ratnawati H, Laksmitawati DR, Setiawan B, et al. Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity. Iranian J Basic Med Sci. 2014;17(9):702–9.
  13. Utami S, Damayanti, NA, Rahmah NA, Nafik S, Arrahmani, BC, Muthmainnah, AS, et al. In vitro studies on antioxidant potential of apple (malus domestica) fructus extract nanoparticle. J Natural Remedies. 202;23(4):1589–98.
  14. Kolayli S, Huseyin S, Zehra C, Oktay Y, Kubra S. Honey shows potent inhibitory activity against the bovine testes hyaluronidase. J Enzyme Inhib Med Chem. 2016;31(4):599–602.
  15. Widowati W, Rani AP, Hamzah RA, Arumwardana S, Afifah E, Kusuma HSW, et al. Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Nat Prod Sci. 2017;23(3):192–200.
  16. Sandhu S, Bansal Y, Silakari O, Bansal G. Coumarin hybrids as novel therapeutic agents. Bioorg Med Chem. 2014;22(15):3806–14. doi:10.1016/j.bmc.2014.05.032
  17. Tu PTB, Tawata S. Antioxidant, antiaging and antimelanogenic propreties of the essential oil from two varieties of Alpinia zerumbet. Molecules. 2015;20(9):16723–40.
  18. Utami S, Laksono SP, Sachrowardi QR, Mustaqimah DN, Endrini S, Damayanti NA, et al. In-vitro studies on antioxidant and antidiabetic potential of Sesoot (Garcinia picrorhiza Miq.) fruit ethanolic extract from Indonesia. Pharmaciana. 2021;11(2):273–82.
  19. Eze FI, Uzor PF, Ikechukwu P, Obi BC, Osadebe PO. In vitro and in vivo models for anti-inflammation: An evaluative review. INNOSC Theranostics and Pharmacological Sciences. 2019;2(2):3–15.
  20. Laksmiani NPL, Nugraha IPW. Depigmentation activity of secang (Caesalpinia Sappan L.) extract through tyrosinase, tyrosinase related protein-1 and dopachrome tautomerase inhibition. Biomed Pharmacol J. 2019;12(2):799–808.
  21. Chantree P, Martviset P, Thongsepee N, Sangpairoj K, Sornchuer P. Anti-Inflammatory effect of garcinol extracted from garcinia dulcis via modulating NF-κB signaling pathway. Nutrients. 2023;15(3):575. doi:10.3390/nu15030575
  22. Rizaldy D, Hartati R, Nadhifa T, Fidrianny I. Chemical compounds and pharmacological activities of Mangosteen (Garcinia mangostana L.)-Update review. Biointerface Res Appl Chem. 2021;12(2021):2503–16.
  23. Tjahjani S, Widowati W, Khiong K, Suhendra A, Tjokropranoto R. Antioxidant properties of Garcinia mangostana L (mangosteen) Rind. Procedia Chem. 2014;13(2014):198–203.
  24. Utami S, Adityaningsari P, Sosiawan I, Endrini S, Sachrowardi QR, Laksono SP, et al. Antioxidants and anticholinesterase activities of the characterized ethanolic of ripe sesoot (Garcinia picrorhiza Miq.) fruit extract (EERS) and xanthone. Trad Med J. 2017;22(3):160–5.
  25. Francik R, Szkaradek N, Oelaszczyk D, Maarona H. Antioxidant activity of xanthone derivatives. Acta Pol Pharm Drug Res. 2016;73(6):1505–9.
  26. Kamal UZ, Sharique AA, Ayesha SA. Mushroom tyrosinase: recent prospects. Hindawi Publishing Corporation Biotechnology Research International. 2016;2016(2016):1–8.
  27. Guo L, Li W, Gu Z, Wang L, Guo L, Ma S, et al. Recent advances and progress on melanin: from source to application. Int J Mol Sci. 2023;24(5):4360. doi:10.3390/ijms240543600
  28. Chatatikun M, Chiabchalard A. Thai plants with high antioxidant levels, free radical scavenging activity, anti-tyrosinase and anti-collagenase activity. BMC Complement Altern Med. 2017;17(2017):487. doi:10.1186/s12906-017-1994-7
  29. Guanhua W, Yue X, Wenjie S, Chuanling S. Lignin as a novel tyrosinase inhibitor: Effects of sources and isolation processes. ACS Sustainable Chem. 2018;6(7):9510–8.
  30. Sahasrabudhe A, Deodhar M. Anti-hyaluronidase, anti-elastase activity of Garcinia indica. Int J Botany. 2010;6(3):299–303.




DOI: https://doi.org/10.15395/mkb.v56.3472

Article Metrics

Abstract view : 186 times
PDF - 75 times

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


 


Creative Commons License
MKB is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

 


View My Stats