Comparison of Antioxidant and Anti-hyaluronidase Potentials of Pineapple Core Extract (Ananas comosus (L.) Merr.) and Luteolin
Abstract
Skin aging processes are divided into chronological aging and premature aging. Premature aging is generally caused by free radicals, from both air pollution and photoaging. Natural compounds from plant extracts are among sources of antioxidants and anti-hyaluronidase which have the ability to prevent antiaging. One of the potential fruits related to antioxidant and antiaging activities is Anana scomosus. A. comosus has a number of phenolic compounds with biological activities. One of the main phenolic compounds in A. comosus is luteolin. The aim of this study was to evaluate the antioxidant and antiaging potentials of Pineapple Core Extract (PCE). This study was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama from August to November 2018. Analysis of antioxidants from PCE and luteolin was carried out using H2O2 scavenging activity assay. The antiaging assay was carried out through inhibition of hyaluronidase enzyme, one of the important enzymes in the aging process. Luteolin had lower IC50 value of H2O2 scavenging activity of around 24.12±3.13 μg/ml, which was better than CPE with IC50 of 304.56±3.76μg/mL. The results of hyaluronidase inhibition activity assay showed that luteolin compound had a lower IC50 value of 67.38±3.99 μg/mL when compared to PCE with an IC50 value of 161.15±1.05 μg/mL. Hence, Luteolin has higher antioxidant and anti-hyaluronidase activities than PCE
Perbandingan Aktivitas Antioksidan dan Antihialuronidase Ekstrak Bonggol Buah Nanas (EBN) dengan Senyawa Luteolin
Terdapat dua jenis proses penuaan kulit, penuaan kronologis dan penuaan dini. Penuaan dini umumnya disebabkan oleh radikal bebas, baik dari polusi udara maupun photoaging. Penggunaan antioksidan dan antihialuronidase untuk mencegah proses penuaan telah banyak diketahui. Ananascomosus merupakan salah satu tanaman yang memiliki potensi sebagai antipenuaan dan antioksidan karena mengandung sejumlah senyawa fenolik yang memiliki berbagai aktivitas biologis. Salah satu senyawa fenolik utama dalam A. comosus adalah luteolin. Tujuan penelitian ini adalah untuk mengevaluasi potensi antioksidan dan anti-aging dari Ekstrak Bonggol Nanas (EBN) dan senyawa pembandingnya yaitu luteolin . Penelitian ini dilakukan di Biomolecular and Biomedicine Research Center, Aretha Medika Utama dari Agustus hingga November 2018. Pengujian antioksidan dari EBN dan luteolin dilakukan menggunakan uji pemerangkapan H2O2. Uji antipenuaan dilakukan melalui uji penghambatan aktivitas hialuronidase, salah satu enzim penting dalam proses penuaan. Hasil penelitian menunjukkan bahwa Luteolin memiliki nilai IC50 aktivitas pemerangkapan H2O2lebih rendah sekitar 24.12±3.13 μg/ml lebih baik daripada EBN dengan IC50 sebesar 304.56±3.76μg/mL. Hasil uji aktivitas penghambatan hialuronidase, senyawa luteolin memiliki nilai IC50 lebih rendah, yaitu sebesar 67,38±3,99 μg/mL dibanding dengan EBN nilai IC50 161,15±1,05μg/mL. Hasil ini menunjukkan bahwa senyawa pembanding luteolin memiliki aktivitas antioksidan dan antihialuronidase yang lebih tinggi daripada EBN.
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Plainfossé H, Burger P, Azoulay S, Landreau A, Verger-Dubois G, Fernandez X. Development of a natural anti-age ingredient based on quercus pubescens willd. leaves extract—a case study. Cosmetics. 2018;5(1):1–21.
Rittié L, Fisher GJ. UV-light-induced signal cascades and skin aging. Ageing Res Rev. 2002;1(4):705–20.
Ndlovu G, Fouche G, Tselanyane M, Cordier W, Steenkamp V. In vitro determination of the anti-aging potential of four southern African medicinal plants. BMC Complement Altern Med. 2013;13(1):304.
Widowati W, Noverina R, Ayuningtyas W, Kurniawan D, Faried A, Laksmitawati D, et al. Reactive Oxygen Species and Aging Mechanism. Reactive oxygen species (ROS) mechanism and role in health and disease. In: Shannon Wilkerson, editor. Reactive oxygen species (ROS): Mechanism and role in helath and disease. New York: Nova Science Publisher; 2018. p. 101–34.
Utami S, Sachrowardi QR, Damayanti NA, Wardhana A, Syarif I, Nafik S, et al. Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging. J Herbmed Pharmacol. 2018;7(2):88–93.
Widowati W, B WJ, Nadya S, Amalia A, Arumwardana S, Kusuma HSW, et al. Antioxidant and antiaging activities of Jasminum sambac extract, and its compounds. J Reports Pharm Sci. 2018;7(3):270–85.
Mukherjee PK, Maity N, Nema NK, Sarkar BK. Bioactive compounds from natural resources against skin aging. Phytomedicine. 2011;19(1):64–73.
Widowati W, Rani AP, Hamzah RA, Arumwardana S, Afifah E, Kusuma HSW, et al. Antioxidant and Antiaging Assays of Hibiscus sabdariffa Extract and Its Compounds. Nat Prod Sci. 2017;23(3):192.
Bhagwat S, Haytowitz DB, Holden JM. USDA database for the flavonoid content of selected foods, Release 3.1. Maryland: Department of Agriculture, Agricultural Research Service Beltsville Human Nutrition Research Center Nutrient Data Laboratory; 2014.
Kang KA, Piao MJ, Ryu YS, Hyun YJ, Park JE, Shilnikova K, et al. Luteolin induces apoptotic cell death via antioxidant activity in human colon cancer cells. Int J Oncol. 2017;51(4):1169–78.
Kasala ER, Bodduluru LN, Barua CC, Gogoi R. Antioxidant and antitumor efficacy of Luteolin, a dietary flavone on benzo(a)pyrene-induced experimental lung carcinogenesis. Biomed Pharmacother. 2016;82:568–77.
Utami S, Adityaningsari P, Sosiawan I, Endrini S, Sachrowardi QR, Laksono SP, et al. Antioxidants and anticholinesterase activities of the characterized ethanolic of ripe sesoot (Garcinia picrorrhiza Miq.) fruit extract (GpKar) and xanthone. Trad Med J. 2017;22(3):160–5.
Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Kusuma HSW, et al. Antioxidant and anti aging assays of oryza sativa extracts, vanillin and coumaric acid. J Nat Remedies. 2016;16(3):88–99.
Mukhopadhyay D, Dasgupta P, Sinha Roy D, Palchoudhuri S, Chatterjee I, Ali S, et al. A Sensitive in vitro spectrophotometric hydrogen peroxide scavenging assay using 1,10-phenanthroline. Free Radicals Antioxidants. 2016;6(1):124–32.
Thring TS, Hili P, Naughton DP. Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complement Altern Med. 2009;9(1):27.
Fidrianny I, Virna V, Insanu M. Antioxidant potential of different parts of bogor pineapple (ananas comosus [l.] merr. var. queen) cultivated in West Java-Indonesia. Asian J Pharm Clin Res. 2018;11(1):129–33.
Mandrone M, Lorenzi B, Venditti A, Guarcini L, Bianco A, Sanna C, et al. Antioxidant and anti-collagenase activity of Hypericum hircinum L. Ind Crops Prod. 2015;76:402–8.
Tu P, Tawata S. Anti-oxidant, anti-aging, and anti-melanogenic properties of the essential oils from two varieties of alpinia zerumbet. Molecules. 2015;20(9):16723–40.
Stern R, Maibach HI. Hyaluronan in skin: aspects of aging and its pharmacologic modulation. Clin Dermatol. 2008;26(2):106–22.
Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: A key molecule in skin aging. Dermatoendocrinol. 2012;4(3):253–8.
DOI: https://doi.org/10.15395/mkb.v51n2.1629
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