Uji Aktivitas Antibakteri Ekstrak Kasar Umbi Sarang Semut (Myrmecodia pendens) Dibanding dengan Klorheksidin terhadap Streptococcus sanguinis

Fathimah Azzahra Attamimi, Rovina Ruslami, Ani Melani Maskoen

Abstract


Streptococcus sanguinis merupakan bakteri pionir penyebab plak gigi. Penggunaan obat kumur klorheksidin merupakan tindakan untuk mengontrol pembentukan plak gigi, namun karena efek sampingnya, klorheksidin tidak dapat digunakan untuk jangka panjang. Umbi sarang semut (Myrmecodia pendens) merupakan tanaman obat yang memiliki banyak aktivitas biologis untuk kesehatan. Penelitian ini bertujuan menguji aktivitas antibakteri ekstrak kasar umbi sarang semut terhadap bakteri S. sanguinis dibandingkan dengan klorheksidin dan pengaruh pemberian konsentrasinya terhadap kematian sel bakteri. Metode penelitian menggunakan uji  Kirby-Bauer untuk menentukan diameter hambat pertumbuhan bakteri, dilanjutkan dengan uji MIC untuk mengetahui nilai MIC dari sampel dan pengaruhnya terhadap kematian sel bakteri. Penelitian dilakukan pada bulan Agustus-Oktober 2015 di Laboratorium Kimia Organik Universitas Padjadjaran Hasil uji Kirby-Bauer ekstrak kasar umbi sarang semut menunjukkan aktivitas antibakteri. Zona hambat yang dihasilkan oleh ekstrak kasar tidak berbeda signifikan dengan klorheksidin (12 mm vs 15 mm, p>0,05). Nilai MIC ekstrak kasar berada diantara 9,77 ppm dan 19,53 ppm, nilai ini lebih kecil dibanding dengan klorheksidin, yaitu sebesar 1,935 ppm. Selain itu, terdapat korelasi positif dan kuat antara konsentrasi ekstrak kasar dan kematian sel bakteri S. sanguinis (r=0,867). Dapat disimpulkan ekstrak kasar umbi sarang semut memiliki efek antibakteri lebih kecil dibanding dengan klorheksidin. Peningkatan konsentrasi eksrak kasar umbi sarang semut memiliki korelasi positif dan kuat terhadap peningkatan kematian sel bakteri S. sanguinis. [MKB. 2017;49(2):94–101]
 
Kata kunci: Antibakteri, ekstrak kasar, klorheksidin, S. sanguinis, umbi sarang semut
 
 
Antibacterial Activity Test of Ant Nest Tuber (Myrmecodia Pendens) Crude Extract against  Streptococcus Sanguinis Compared to Chlorhexidine
 
Streptococcus sanguinis is a pioneer bacterium that causes dental plaque formation. The use of chlorhexidine mouthwash is one of the treatments to control dental plaque. However, due to its side effects, chlorhexidine cannot be used in a long period. Ant nest tuber (Myrmecodia pendens) is one of the medicinal plants with variousbiological activities that are beneficial to human health. The purpose of this study was to examine the antibacterial activity of ant nest tuber crude extract against bacteria S. sanguinis compared to chlorhexidine and measure the effect of increased concentration on bacterial cell death. This study was conducted using Kirby-Bauer method to measure the diameter of bacterial growth inhibition in August–October 2015 at the Organic Chemistry Laboratory of Universitas Padjadjaran. The MIC was then measured to determine the MIC values of the sample and the effect on bacterial cell death. Kirby-Bauer test showed that the inhibition zone produced by crude extract was not significantly different from the one produced by chlorhexidine (12 mm vs 15 mm, p>0.05) when both were used to inhibith the growth of S. sanguinis. The MIC value of crude extract was between 9.77 ppm and 19.53 ppm, smaller than the MIC value of the chlorhexidine of 1.93 ppm. In addition, there was a positive and strong correlation (r=0.867) between the concentration of crude extract and S. sanguinis cell death.  Hence,  crude extract from ant nest tuber has a lower antibacterial effect than chlorhexidine in inhibiting the growth of S. sanguinis. Additionally, there is a strong correlation between the increased concentration of crude extract and increased S. sanguinis cell death. [MKB. 2017;49(2):94–101]
 
Key words: Antibacterial, ant nest tuber, chlorhexidine, crude extract, S. sanguinis
 

Keywords


Antibakteri, ekstrak kasar, klorheksidin, S. sanguinis, umbi sarang semut

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References


Lockhart PB, Bolger AF, Papapanou PN, Osinbowale O, Trevisan M, Levison ME, dkk. Periodontal disease and atherosclerotic vascular disease: does the evidence support an independent association? A scientific statement from the American Heart Association. Circulation. 2012;125(20):2520–44.

Xu P, Alves JM, Kitten T, Brown A, Chen Z, Ozaki LS, dkk. Genome of the opportunistic pathogen Streptococcus sanguinis. J Bacteriol. 2007;189(8):3166–75.

Ge X, Kitten T, Chen Z, Lee SP, Munro CL, Xu P. Identification of Streptococcus sanguinis genes required for biofilm formation and examination of their role in endocarditis virulence. Infect Immunol. 2008;76(6):2551–9.

Okahashi N, Nakata M, Terao Y, Isoda R, Sakurai A, Sumitomo T, dkk. Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation. Microb Pathog. 2011;50(3):148–54.

Dutt P, Rathore PK, Khurana D. Chlorhexidine-An antiseptic in periodontics. IOSR-JDMS. 2014;13(9):85–8.

Hamsar M, Mizaton H. Potential of ant-nest plants as an alternative cancer treatment. J Pharm Res. 2012;5(6):3063–6.

Setyani W. Isolasi dan identifikasi senyawa antimikroba dari umbi sarang semut (Myrmecodia tuberosa (non Jack.) BI) [tesis]. Yogyakarta: Universitas Gadjah Mada; 2011.

Efferth T, Koch E. Complex interactions between phytochemicals. The multi-target therapeutic concept of phytotherapy. Curr Drug Targets. 2011;12(1):122–32.

Abdul-Aziz MH, Lipman J, Mouton JW, Hope WW, Roberts JA. Applying pharmacokinetic/pharmacodynamic principles in critically ill patients: optimizing efficacy and reducing resistance development. Semin Respir Crit Care Med. 2015;36(1):136–53.

Richards G, Joubert I, Brink A. Optimising the administration of antibiotics in critically ill patients: continuing medical education-article. S Afr Med J. 2015;105(5):419–24.

Supriatno. Antitumor activity of Papua’s Myrmecodia pendans in human oral tongue squamous cell carcinoma cell line through induction of cyclin-dependent kinase inhibitor p27Kip1 and suppression of cyclin E. J Cancer Res Ther. 2014;2:48–53.

Pandia DMH, Wibriansyah A, Pratiwi W, Priadinata AF, Sari W. Uji toksisitas akut ekstrak etanol sarang semut (myrmecodia pendens) Kalimantan pada mencit (Mus musculus) Swiss. Majalah Kesehatan Pharmamedika. 2012;3(1):1-5;

Soeksmanto A, Simanjuntak P, Subroto MA. Uji toksisitas akut ekstrak air tanaman sarang semut (myrmecodia pendans) terhadap histologi organ hati mencit. Jurnal Natur Indonesia. 2010;12(2):152–5.

Woodward K. Veterinary pharmacovigilance. Part 4. Adverse reactions in humans to veterinary medicinal products. J Vet Pharmacol Ther. 2005;28(2):185–201.

Engida AM, Kasim NS, Tsigie YA, Ismadji S, Huynh LH, Ju Y-H. Extraction, identification and quantitative HPLC analysis of flavonoids from sarang semut (Myrmecodia pendan). Industrial Crops Products. 2013;41:392–6.

Hajifattahi F, Moravej-Salehi E, Taheri M, Mahboubi A, Kamalinejad M. Antibacterial Effect of hydroalcoholic extract of punica granatum Linn. Petal on common oral microorganisms. Int J Biomaterials. 2016; 2016(2):1–6.

Razak FA, Othman RY, Rahim ZHA. The effect of Piper betle and Psidium guajava extracts on the cell-surface hydrophobicity of selected early settlers of dental plaque. J Oral Sci. 2006;48(2):71–5.

Nair MS, Joseyphus RS. Synthesis and characterization of Co (II), Ni (II), Cu (II) and Zn (II) complexes of tridentate Schiff base derived from vanillin and DL-α-aminobutyric acid. Spectrochimica Acta Part A: Molecular Biomolecular Spectroscopy. 2008;70(4):749–53.

Iwasaki Y, Hirasawa T, Maruyama Y, Ishii Y, Ito R, Saito K, dkk. Effect of interaction between phenolic compounds and copper ion on antioxidant and pro-oxidant activities. Toxicol In Vitro. 2011;25(7):1320–7.




DOI: https://doi.org/10.15395/mkb.v49n2.1053

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