Potensi Ekstrak Etanol Buah Citrullus lanatus sebagai Agen Imunosupresi melalui Pengamatan Histologi Limpa Mencit BALB/c
Abstract
Citrullus lanatus mengandung flavonoid yang berpotensi sebagai agen imunosupresi. Penelitian ini bertujuan mengkaji perubahan diameter pulpa alba limpa mencit BALB/c yang diinduksi ovalbumin (OVA) setelah pemberian ekstrak etanol buah C. lanatus. Jenis penelitian ini adalah eksperimental dengan post-test only control group design. Sebanyak 30 ekor mencit BALB/c jantan dibagi menjadi enam kelompok, yaitu tiga kelompok kontrol (normal, negatif, dan metilprednisolon) dan tiga kelompok perlakuan ekstrak etanol C. lanatus dosis 175 mg/kgBB, 350 mg/kgBB, dan 700 mg/kgBB per hari. Ekstrak dan Metilprednisolon diberikan selama 28 hari, sedangkan OVA diberikan pada hari ke-15 dan ke-22 secara intraperitoneal dan per oral pada hari ke-23 sampai dengan hari ke-28. Pada hari ke-29, mencit dikorbankan dan organ limpa diambil untuk dibuat sediaan histologi dengan teknik pewarnaan HE. Efek imunosupresi dinilai dari rata-rata diameter pulpa alba limpa. Data dianalisis dengan uji nonparametrik Kruskal-Wallis dilanjutkan uji Mann-Whitney. Diameter pulpa alba limpa mengalami peningkatan setelah diinduksi OVA, pemberian ekstrak etanol C. lanatus dosis 700 mg/kgBB dan metilprednisolon menyebabkan penurunan diameter pulpa alba limpa secara bermakna (p<0,05), tetapi ekstrak etanol C. lanatus dosis 175 dan 350 mg/kgBB tidak menurunkan diameter pulpa alba limpa. Ekstrak etanol C. lanatus dosis 700 mg/kgBB mampu menurunkan diameter pulpa alba pada limpa mencit BALB/c yang diinduksi OVA sehingga berpotensi sebagai agen imunosupresi.
Kata kunci: Agen imunosupresi, citrullus lanatus, mencit BALB/c, ovalbumin, pulpa alba limpa
Citrullus lanatus Ethanol Extract Potential as Immunosupressive Agent based on Spleen Histology Observation in BALB/c Mice
Citrullus lanatus contains flavonoid which is known as an immunosuppresion agent. This study aimed to evaluate BALB/c mice spleen white pulp diameter after Ovalbumin (OVA) induction and administration of C. lanatus ethanol extract. This was a post-test only control group experimental study. Thirty male BALB/c mice were divided into six groups consisting of three control groups (normal, negative, and methylprednisolone) and three C. lanatus ethanol extract treatment groups (175 mg/kg bw, 350mg/kg bw, and 700mg/kg bw dose per day). The ethanol extract and methylprednisolone were administered to the respective groups for 28 consecutive days, while the OVA was given intraperitoneally on day 15 and day 22 and orally from day 23 to day 28. On day 29, the mice were sacrificed and their spleen were processed histologically using the HE staining technique. The immunosupression effect was assessed by measuring the white pulp spleen diameter. Data were analyzed using nonparametric Kruskal-Wallis test, followed by Mann-Whitney test. After OVA induction, the diameter of white pulp spleen was found to increase. Significant decrease in the spleen white pulp diameter (p<0.05) was seen in the 700 mg/kgbw C. lanatus ethanol extract group and methylprednisolone group. No shortening of the spleen white pulp diameter was seen in 175 mg/kg bw and 350 mg/kgbw C. lanatus ethanol extract groups. Hence, 700 mg/kgbw C. lanatus ethanol extract can decrease the spleen white pulp diameter in an OVA-induced BALB/c mice and may have a potential to be used as an immunosuppressive agent.
Key words: BALB/c mice, citrullus lanatus, immunosuppresion agent, ovalbumin, white pulp of spleen
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Valenta R, Hochwallner H, Linhart B, Pahr S. Food allergies: the basics. Gastroenterology. 2015;148(6):1120–31.
Baena-Cagnani CE, Teijiro A. Role of food allergy in asthma in childhood. Curr Opin Allergy Clin Immunol. 2001;1:145-9.
Bronte V, Pittet MJ. The Spleen in local and systemic regulation of immunity. Immunity. 2013;39(5):806–18.
Husain M, Makiyah SNN. Pengaruh pajanan gelombang telepon seluler terhadap struktur histologi limpa pada mencit (Mus musculus). J Kedokteran Yarsi. 2012;20(3):167–73.
Makiyah SNN, Iszamriach R, Nofariyandi, A. Paparan ultraviolet c meningkatkan diameter pulpa alba limpa dan indeks mitotik epidermis kulit mencit. Jurnal Kedokteran Brawijaya. 2014;28(1):17–21.
Deshmukh, CD, Jain A, Tambe MS. Phytochemical and pharmalogical profile of Citrullus lanatus (THUNB). Biolife. 2015; 3(2):483–8.
Durga M, Nathiya S, Devasena T. Multifarious actions of dietary flavonoids – implications in cancer and cataract. Int J Pharm Bio Sci 2014;5(2):404–16.
Hidayati NA, Listyawati S, Setyawan AD. Kandungan kimia dan uji antiinflamasi ekstrak etanol Lantana camara L. pada tikus putih (Rattus norvegicus L.) jantan. Bioteknologi. 2008;5(1):10–7.
Kantham, S. Influence of Carica papaya linn extracts on paracetamol and thioacetamide induced hepatic damage in rats. Internet J Pharmacol. 2009;9(1):1–5.
Makiyah SNN, Noor Z, Djati MS, Rifa’i M, Widodo. Ethanol extracts of tubers Dioscorea alata l. as antiallergic agents on mice balb/c induced with ovalbumin. Int J Pharma Bio Sci. 2014;5(3):214–20.
Anggarasari Y. Pengaruh pemaparan karbofuran terhadap gambaran diameter pulpa putih limpa mencit (Mus musculus). Veterinaria Medika. 2014;7(2):100–5.
Prasetyo D. Efek Pemaparan ovalbumin aerosol terhadap eosinofilia bronkus pada mencit balb/c. Nexus Medicus. Surakarta: Fakultas Kedokteran Universitas Sebelas Maret; 2007.
Baratawidjaja KG. Imunologi dasar. Edisi ke-9. Jakarta: Balai Penerbit FKUI; 2009.
Novia A. Pengaruh pemberian ekstrak etanol umbi bawang dayak (Eleutherine bulbosa (Mill.) Urb.) secara oral pada mencit balb/c terhadap pencegahan penurunan jumlah sel yang terekspresi IFN-γ dan peningkatan jumlah sel yang terekspresi CD14. J Biosains Pascasarjana UNAIR. 2015;17(3):1–16.
Sudir J. Efek kortikosteroid terhadap metabolisme sel; dasar pertimbangan sebagai tujuan terapi pada kondisi akut maupun kronik. Dexa Media. 2007;20(2):77–80.
García-Lafuente A1, Guillamón E, Villares A, Rostagno MA, Martínez JA. Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm Res. 2009;58(9):537–52.
Rang HP, Dale MM, Ritter JM, Flower RJ. Rand and dale’s pharmacology. Edisi ke-7. London: Churchill Livingstone; 2007.
Gupta A., Khamkar PR, Chaphalkar SR. Inhibition of nitric oxide production and pro-inflammatory cytokines by aqueous extract of terminalia arjuna in human peripheral blood mononuclear cells. Int J Pharm Biol Sci Arch. 2014;2:29–33.
Gajalakshmi S, Vijayalakshmi S, Devirajeswari. Echinacea purpurea–a potent immunostimulant. J Pharm Sci Rev Res. 2012;14(2):47–52.
Makiyah A, Husin U, Sadeli R. Efek imunostimulasi ekstrak etanol umbi iles-iles terhadap aktivitas fagositosis sel makrofag pada tikus putih strain wistar yang diinokulasi Staphylococcus aureus. MKB. 2016;48(2):68–77.
DOI: https://doi.org/10.15395/mkb.v49n4.1024
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