Gambaran Pola Kuman pada Bilah Laringoskop di Ruang Operasi Rumah Sakit Dr. Hasan Sadikin Bandung

Abstract

Kebiasaan cara membersihkan bilah laringoskop dapat menyebabkan komplikasi karena potensial kontak dengan mikro-organisme patogen dan dapat menyebabkan infeksi nosokomial. Tujuan penelitian adalah mengetahui gambaran kuman pada bilah laringoskop dengan metode pembersihan yang dilakukan di Rumah Sakit Dr. Hasan Sadikin (RSHS) Bandung. Penelitian dilakukan menggunakan metode deskriptif observasional dengan cara melakukan pemeriksaan kultur kuman pada 19 bilah laringoskop sebelum digunakan dan yang sudah dibersihkan sesudah tindakan laringoskopi, serta 14 sumber air untuk membersihkan bilah laringoskop di ruang operasi RSHS Bandung pada tanggal Desember 2014–Januari 2015. Hasil penelitian ini ditemukan gambaran pola kuman komensal pada bilah laringoskop yang digunakan sebelum tindakan laringoskopi, yaitu Bacillus spp. 10 dari 19. Ditemukan gambaran pola kuman patogen pada bilah laringoskop yang sudah dibersihkan setelah tindakan laringoskopi di ruang operasi, yaitu Candida non albicans 1 dari 19, Acinobacter baumanii dan Staphylococcus haemolyticus 1 dari 19, serta Klebsiella pneumoniae dan Pseudomonas aeruginosa 1 dari 19. Simpulan, ditemukan kuman patogen pada bilah laringoskop yang sudah dibersihkan sesudah tindakan laringoskopi dan air yang digunakan untuk membersihkan bilah laringoskop di Rumah Sakit Dr. Hasan Sadikin Bandung.

Kata kunci: Bilah laringoskop, infeksi nosokomial, laringoskopi, pola kuman

Bacterial Mapping of Laryngoscope Blade at the Operating Theater of Dr. Hasan Sadikin General Hospital Bandung

Abstract

The habit of cleaning laryngoscope blades can cause complications due to potential contact with patogenic microorganisms that may cause nosocomial infections. The aim this study was to determine the microbial patterns on the laryngoscope blades cleaned using the cleaning methods applied in Dr. Hasan Sadikin General Hospital (RSHS) Bandung. This study was conducted using the descriptive observational method by taking samples of bacterial culture from 19 laryngoscope blades before laryngoscopy procedures and 19 cleaned laryngoscope after laryngoscopy, as well as from the 14 water sources that were used for cleaning laryngoscope blades in the operating theaters of RSHS Bandung in December 2014–January 2015. The results of this study revealed a commensal microbial pattern of Bacillus spp. on 10 of 19 laryngoscope blades before they were used in laryngoscopy precedures. It was revealed that among the laryngoscopes blade that had been cleaned after laryngoscopy in the operating room. 1 of 19 was found to have a non-Candida albicans pattern, 1 of 19 had Acinobacter baumannii and Staphylococcus haemolyticus pattern, and 1 of 19 had Klebsiella pneumoniae and Pseudomonas aeruginosa pattern. Hence, it can be concluded that pathogenic germs are found in laryngoscope bplades that have been cleaded after laryngoscopy and water that is used to clean them in Dr. Hasan Sadikin General Hospital Bandung.

Key words: Bacterial mapping, direct laryngoscopy, laryngoscope blades, nosocomial infections

DOI: 10.15851/jap.v4n3.899