Durian Consumption Effect on the Plasma Malondialdehyde Level as Biomarker of Stress Oxidative in Rats

Anugrah Aulia Ulil Amri, Ani Melani Maskoen, Syarief Hidayat


Background: Excessive consumption of durian (Durio zibethinus Murray) in Indonesia is often connected with its effect on health. This study aims to understand the effect of durian consumption to malondialdehyde (MDA) in plasma as oxidative stress biomarker.

Methods: The study used an experimental research design on animal models, in the Biochemistry and Molecular Biology Department, Faculty of Medicine, Universitas Indonesia, July–August 2012. Thirty two Sprague-Dawley rats were used, divided into four groups: control, treatment week 1, 2, and 3. Each treatment group was given 20 gram durian fruit diluted with water until 20 ml volume per oral, divided into two doses (10 ml each) with 4 hours interlude between doses for 1 week, 2 weeks, and 3 weeks. All groups got normal diet and water ad libitum. Plasma MDA level was measured by TBARS method, then analyzed using Kurskal-Wallis and Mann-Whitney tests.

Results: Seventeen samples were successfully decapitated (5 for control; 6 for week 1; 3 for week 2; 3 for week 3). Average plasma MDA level for control treatment week 1, 2 and 3 groups were 0.707 nmol/ml, 0.432 nmol/ml, 0.312 nmol/ml, and 0.746 nmol/ml respectively. Data was significant (p<0.05) with p=0.02. Compared with control group, a significant increase occurred in week 1 and 2 groups with p=0.028 and p=0.025 respectively.

Conclusions: Results of durian consumption show MDA level significantly decreases in week 1 and 2. However, MDA level dramatically increases exceeding control group level in week 3. [AMJ.2016;3(1):22–8]


DOI: 10.15850/amj.v3n1.695


Durian, malondialdehyde, oxidative stress

Full Text:



Avila AP, Toledo F, Park YS, Jung ST, Kang SG, Heo BG, et al. Antioxidant properties of durian fruit as influenced by ripening. LWT-Food Sci Tech. 2006;41(10):2118–125.

Leontowicz H, Leontowicz M, Haruenkit R, Poovarodom S, Jastrzebski Z, Drzewiecki J, et al. Durian (Durio zibethinus Murr.) cultivars as nutritional supplementation to rat’s diets. Food Chem Toxicol. 2008;46(2):581–89.

Toledo F, Avila AP, Park YS, Jung ST, Kang SG, Heo BG, et al. Screening of the antioxidant and nutritional properties, phenolic contents and proteins of five durian cultivars. Int J Food Sci Nut. 2008;59(5):415–27.

Haruenkit R, Poovardom S, Vearasilp S, Namiesnik J, Sliwka-Kaszynska M, Park Y, et al. Comparison of bioactive compounds, antioxidant and antiproliferative activities of Mon Thong durian ripening. Food Chem. 2010;118(3):540–7.

Haruenkit R, Poovarodom S, Leontowicz H, Leontowicz M, Sajewicz M, Kowalska T, et al. Comparative study of health properties and nutritional value of durian, mangosteen, and snake fruit: Experiments in vitro and in vivo. J Agric Food Chem. 2007;55(14):5842−9.

Phutdhawong W, Kaewkong S, Buddhasukh D. GC–MS analysis of fatty acids in Thai durian aril. Chiang Mai J Sci. 2005;32(2):169–72.

Kumar V, Abbas AK, Fausto N, editors. Robbins and Cotran Pathologic Basis of Disease, 8thed. Philadelphia: Elsevier Inc; 2005.

Repetto M, Semprine J, Boveris A. Lipid peroxidation: Chemical mechanism, biological implications and analytical determination. In: Catala A, editor. Lipid peroxidation. Rijeka: InTech; 2012. p. 1−21.

Purnomo Suryohudoyo. Oksidan, antioksidan dan radikal bebas. In: Ilmu kedokteran molekuler. Kapita Selekta. Jakarta: Sagung Seto; 2000. p.31−46.

Linsley MD, Ekinci FJ, Ortiz D, Rogers E, Shea TB. Monitoring thiobarbituric acid-reactive substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. J Neurosci Methods 2005;141(2):219−22

Cao G, Sofic S, Prior RL. Antioxidant and prooxidant behavior of flavonoids: Structure-Activity relationships. J Free Rad Biol Med 2014;22(5):749−60.

Amid BT, Mirhosseini H, Kostadinović S. Chemical composition and molecular structure of polysaccharide-protein biopolymer from Durio zibethinus seed: Extraction and purification process. Chem Cent J. 2012;6(1):117.

Sengupta P. The laboratory rat: Relating its age with human’s. Int J Prev Med. 2013;4(6):624–6.

Article Metrics

Abstract view : 611 times
PDF - 254 times

 This Journal indexed by




Creative Commons License
AMJ is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License


View My Stats