Effect of Carica Papaya L Leaf Infusion on Transporting Glucose across Small Intestine Cell Membrane of Wistar Rats Model

Srisathis Renganathan, Vycke Yunivita, Anna Martiana S, Nida Suraya


Background: Carica papaya L leaf is one of the common types of plant that has been used as treatment for diabetes mellitus (DM). The leaf possibly contain quercetin, which may inhibit glucose transport through cell membrane of small intestine. This study was conducted to determine the effect of Carica papaya L leaf infusion on glucose transport in Wistar rat model.

Methods: A one-group pretest-posttest explorative study was designed using perfusion method. Nine male rats were fasted for 18-24 hours before dissected. A 25 mL glucose solution was given as early control, before Carica papaya L leaf infusion treatment A 25 mL glucose solution was later given again after Carica papaya L leaf infusion as late control. Artificial perfusion equipment had been used to maintain the back and forth flow of glucose in the small intestine. Sample was taken every 15 minutes for one hour for each treatment.

Results: Carica papaya L leaf infusion did not decrease the concentration of glucose absorption significantly compared to early control. The mean absorption of glucose in early control was 85.39+4.42 mg/dl and during treatment was 84.51+4.20 mg/dL.

Conclusions: Carica papaya L leaf infusion does not have significant inhibiting effect on glucose transport across small intestine cell membrane. Further explorative study is required.


Carica papaya L leaf, cell membrane, glucose transport, small intestine, Wistar rats

Full Text:



Maharani U, German M. Pancreatic hormones and diabetes mellitus. In: Gardner D, Shoback D, editors. Greenspan’s basic and clinical endocrinology. 8th ed. San Francisco: McGraw Hill; 2007. p. 661−747.

PERKENI. Konsensus Pengendalian dan Pencegahan Diabetes Mellitus Tipe 2 di Indonesia. 8th ed. Jakarta: PERKENI; 2015. p. 1-82

Molina PE. Endocrine physiology. 2nd ed. New York: McGraw-Hill Medical; 2006. p. 173−8.

Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam. Indian herbs and herbal drugs used for the treatment of diabetes. J Clin Biochem Nutr. 2007;40(3):163−73.

Indran M, Mahmood AA, Kuppusamy UR. Protective effect of Carica papaya L leaf extract against alcohol induced acute gastric damage and blood oxidative stress in rats. West Indian Med J.2008;57(4):323−6.

Lim TK. Edible medicinal and non-medicinal Plants. Carica Papaya. 2nd ed. New York: Springer;2012. p. 693−717.

Canin A, Alesiani D, D’Arcangelo G, Tagliatesta P. Gas chromatography–mass spectrometry analysis of phenoliccompounds from Carica papaya L. leaf . J Food Comp Anal. 2007;20(7):580 ̶ 90.

Abdullah NR., Afzan A, Halim SZ, Rashid BA, Semail RH, Abdullah N, et al. Repeated dose 28-days oral toxicity study of Carica papaya L. leaf extract in Sprague Dawley Rats. Molecules. 2012;17(4):4326−42.

Gray N. Phenolic compounds may regulate glucose transport: Study. 2010;[cited 2013 February 24] Available from: http://www.drpressman.com/phenolic-compounds-may-regulate-glucose-transport-study/.

Marsongkohadi, Sudigdo. Effect of herbal leaf infusion on transporting glucose across small intestine cell membrane of Wistar rat [dissertation]. Bandung: Institut Teknologi Bandung;1988.

Juarez-Rojop IE, Daaz-Zagoya JC, Ble-Castillo JL, Miranda-Osorio PH, Castell-Rodraguez AsE, Tovilla-Zarate CA, et al. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2012;12(1):236.

Maniyar Y, Bhixavatimath P. Antihyperglycemic and hypolipidemic activities of aqueous extract of Carica papaya Linn. leaves in alloxan-induced diabetic rats. J Ayurveda Integr Med. 2012;3(2):70−4.

DOI: https://doi.org/10.15850/amj.v6n1.512

Article Metrics

Abstract view : 434 times
PDF - 292 times

 This Journal indexed by




Creative Commons License
AMJ is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License


View My Stats