Anti-hyperglycemic Effect of Psidium guajava Leaf Infusion
Abstract
Background: Prevalence of diabetes mellitus is estimated to increase annually. Numerous people use traditional medicine, such as the Poidium guajava leaf to prevent this disease. This study aimed to analyse the effect of Psidium guajava leaf to inhibit glucose absorption in intestine epithelial membrane of wistar rats.
Methods: This laboratory experimental study used 5 wistar rats as subjects in the Laboratory of Biochemistry at Padjadjaran University from 10−26 October 2012. All rats were given three solutions by in situ perfusion
method. The first was 25 ml 3.0 X 10-3 M glucose solution, the second was 25 ml 3.0 X 10-3 M glucose solution with 1 ml Psidium guajava infusion added, and the third was 25 ml 3.0 X 10-3 M glucose solution.
The sample from each solution was taken five times at 0, 15, 30, 45, and 60 minutes after the solutions were given. The spectrophotometer was to quantify the concentration of glucose from the samples. Data were analyzed using Friedman and Wilcoxon test.
Results:The means of glucose concentration for each solution from the first solution to the third were 6.126 mg/dl, 2.447 mg/dl, and 5.345 mg/dl. The probability value showed significant difference between the first and second solutions (p ≤ 0.05).
Conclusions: Psidium guajava leaf infusion can inhibit glucose absorption in wistar rat intestine and the effect is reversible. [AMJ.2015;2(4):546–9]
DOI: 10.15850/amj.v2n4.508
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Gardner DG, Shoback DM, Greenspan FS.
Greenspan’s basic & clinical endocrinology.
th ed. San Francisco: McGraw-Hill
Medical; 2007.
Wild S, Roglic G, Green A, Sicree R, King H.
Global prevalence of diabetes estimates
for the year 2000 and projections for 2030.
Diabetes Care. 2004;27(5):1047−53.
Willett W, Manson J, Liu S. Glycemic index,
glycemic load, and risk of type 2 diabetes.
Am J Clin Nutr. 2002;76(1):274S−80S.
Gutierrez RM, Mitchell S, Solis RV. Psidium
guajava: a review of its traditional uses,
phytochemistry and pharmacology. J
Ethnopharmacol. 2008;117(1):1−27.
Deguchi Y, Miyazaki K. Anti-hyperglycemic
and anti-hyperlipidemic effects of guava
leaf extract. Nutr Metab. 2010;7(1):9.
Fortress Diagnostic. Glucose (GOD-PAP)
liquid stable. Antrim: Fortress Diagnostics
Limited; 2009. p. 1−2.
Ojewole JAO. Hypoglycemic and
hypotensive effects of Psidium guajava
Linn. (Myrtaceae) leaf aqueous extract.
Methods Find Exp Clin Pharmacol. 2005;
(10):689−95.
Mukhtar HM, Ansari SH, Ali M, Naved T,
Bhat ZA. Effect of water extract of Psidium
guajava leaves on alloxan-induced diabetic
rats. Pharmazie. 2004;59(9):734−5.
Wang B, Liu HC, Ju CY. Study on the
hypoglycemic activity of different
extracts of wild Psidium guajava leaves in
Panzhihua Area. Sichuan Da Xue Xue Bao
Yi Xue Ban. 2005;36(6):858−61.
Deguchi Y. Effect of guava tea on
postprandial blood glucose and diabetes.
Assoc J Jpn Soc Med Use Funct Foods.
;3(6):439−45.
Wang B, Liu HC, Hong JR, Li HG, Huang
CY. Effect of Psidium guajava leaf extract
on alpha-glucosidase activity in small
intestine of diabetic mouse. Sichuan Da Xue
Xue Bao Yi Xue Ban. 2007;38(2):298−301.
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