Thawing Time Difference between Fresh Frozen Plasma Using Ziplock Plastic and non-Ziplock Plastic in Blood Transfusion Unit Dr. Hasan Sadikin General Hospital Bandung, Indonesia

Rima Rinanty, Leni Lismayanti, Raja Iqbal Mulya Harahap

Abstract


Background: The quality of fresh frozen plasma (FFP) in a clinical setting depends on thawing time. Thawing using a water bath is often used in blood transfusion units because it is easy to perform, affordable, and easy to look for. Protective plastics (Ziplock and non-Ziplock) are used to reduce the risk of contamination,. This study aimed to determine whether there is a difference in thawing time between FFP using Ziplock plastic and non-Ziplock plastic.

Method: This experimental design was conducted in the Blood Bank Unit of Hasan Sadikin Hospital from June-August 2021. Samples were divided into two groups, namely thawing using Ziplock and non-Ziplock. Each group consisted of volume 160-200 ml and 201-240 ml.

Results: The total samples were 24 FFP bags. In the group of the bag 160-200 ml, the median thawing time using Ziplock plastic was 8 minutes (8-16 minutes), non-Ziplock was 15 minutes (8-16 minutes) (p value 0,111), whereas in a group of bags with volume 201-240 ml, the median thawing time using Ziplock was 15 minutes (8-28 minutes), non-Ziplock was 20 minutes (14-30 minutes) (p- value 0,332). Although there was a time difference in both groups, the difference was non-significant.

Conclusion: The thawing time between the small bag with a volume of 160-200 ml and the larger volume of 201-240 ml shows no difference. Ziplock plastic can be used to reduce the risk of contamination.


Keywords


FFP, non-Ziplock plastic,thawing time, water bath, Ziplock plastic

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References


  1. Uhl L. Clinical use of plasma components. UpToDate [Internet]. [cited 2022 June 10]. Available from: https://www.uptodate.com/contents/clinical-use-of-plasma-components.
  2. O’Shaughnessy DF, Atterbury C, Maggs PB, Murphy M, Thomas D, Yates S, et al. Guidelines for the use of fresh-frozen plasma, cryoprecipitate and cryosupernatant. Br J Haematol. 2004;126(1):11–28.
  3. Khawar H, Kelley W, Stevens JB, Guzman N. Fresh frozen plasma (FFP). Treasure Island (FL): StatPearls Publishing; 2021.[cited 2021 April 11]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK513347/.
  4. Veera RRL, Schneider D, Pickens PV. Fresh frozen plasma (FFP) usage and appropriateness in adult medical in-patients: a retrospective audit. Blood. 2012;120(21):4375.
  5. Cardigan R, Green L. Thawed and liquid plasma–what do we know? Vox Sang. 2015;109(1):1–10.
  6. Meledeo MA, Peltier GC, McIntosh CS, Corley JB, Bynum JA, Cap AP. Field-expedient thawing of fresh-frozen plasma. Transfusion . 2020;60(Suppl 3):S87–95.
  7. Churchill WH, Schmidt B, Lindsey J, Greenberg M, Boudrow S, Brugnara C. Thawing fresh frozen plasma in a microwave oven: a comparison with thawing in a 37 °C waterbath. Am J Clin Path. 1992;97(2):227–32.
  8. Khandpur RS. Plasma thawing equipment. In: Khandpur RS, editor. Compendium of biomedical instrumentation. 1st Ed. Hoboken: John Wiley & Son; 2019. p.1537–9.
  9. Retno D, Zainal AR, Machsoos BD, Hermanto DH, Wardhani SO. Perbedaan kualitas fresh frozen plasma yang dicairkan dengan metode konvensional dan dengan metode alat FFP thawer. J Peny Dalam. 2012;13(1):21–7.
  10. Pinki S, Mohan G, Rafi A, Innah S, Thomas T. Rapid dry plasma thawing system: An alternative to conventional thawing baths. Asian J Transfus Sci. 2017;11(2):147–50.
  11. Heger A, Pock K, Römisch J. Thawing of pooled, solvent/detergent-treated plasma octaplasLG®: validation studies using different thawing devices. Transfus Med Hemother. 2017;44(2):94–8.
  12. Sojiphan K, Iverson A, Kim S. Materials selection analysis: bag for viable blood storage [Internet]. 2004. [cited 2021 April 24]. Available from: https://www.researchgate.net/publication/291766516_Materials_Selection_Analysis_Bag_for_Viable_Blood_Storage.
  13. Zahra NM, Siswanto S, Widiyanti P. The role of chitosan on polyvinyl chloride (PVC)-glycerol biocomposites for blood bag application. JBBBE. 2018;37:94–106.
  14. Platton S, Elegbe O, Bower L, Cardigan R, Lancut J, McCullagh J, et al. Thawing times and hemostatic assessment of fresh frozen plasma thawed at 37°C and 45°C using water-bath methods. Transfusion. 2019;59(11):3478–84.
  15. Chavan SK. Determination of rate and analysis of reasons for discarding blood and blood components in a blood bank of tertiary care hospital:a retrospective study. Int J Res Med Sci. 2017;5(3):1111–5.
  16. Green L, Bolton-Maggs P, Beattie C, Cardigan R, Kallis Y, Stanworth SJ, et al. British Society of Haematology Guidelines on the spectrum of fresh frozen plasma and cryoprecipitate products: their handling and use in various patient groups in the absence of major bleeding. Br J Haematol. 2018;181(1):54–67.
  17. Plotz RD, Ciotola RT. Thawing of fresh-frozen plasma at 45 °C versus 37 °C: comparison using satellite packs of the same donor units. Am J Clin Pathol. 1988;89(3):381–4.
  18. Dhantole L, Dubey A, Sonker A. A study on factors influencing the hemostatic potential of fresh frozen plasma. Asian J Transfus Sci. 2019;13(1):23–9.
  19. Castillo B, Dasgupta A, Klein K, Tint H, Wahed A. Transfusion medicine for pathologists: a comprehensive review for board preparation, certification, and clinical practice. 1st Ed. Amsterdam: Elsevier; 2018.
  20. Ihwah A, Deoranto P, Wijana S, Dewi IA. Comparative study between Federer and Gomez method for number of replication in complete randomized design using simulation: study of Areca Palm (Areca catechu) as organic waste for producing handicraft paper. IOP Conf Ser Earth Environ Sci. 2018;131(1):012049.
  21. Agrawal A, Tiwari AK, Mehta N, Bhattacharya P, Wankhede R, Tulsiani S, et al. ABO and Rh (D) group distribution and gene frequency; the first multicentric study in India. Asian J Transfus Sci. 2014;8(2):121–5.
  22. Simpson V. World population by percentage of blood types-WorldAtlas [Internet]. 2020. [Cited 2022 May 1]. Available from: https://www.worldatlas.com/articles/what-are-the-different-blood-types.html.
  23. Virtanen AJ, Goedeken DL, Tong CH. Microwave assisted thawing of model frozen foods using feed-back temperature control and surface cooling. J Food Sci. 2006;62(1):150–4.




DOI: https://doi.org/10.15850/amj.v10n3.2795

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