Preservation in Paraffin Oil: Alternative for Fungi Preservation in Simple Laboratory Concept

Sem Samuel Surja, Gabi Vania Sally, Favian Sergius Ruby Hartoyo, Sandy Vitria Kurniawan, Yanto Budiman


Background: A good collection of fungi culture can be maintained with good preservation methods. With appropriate methods, preservation can be performed in low-budget laboratories, such as with periodic subculture and preservation in either sterile water or in paraffin oil. The aim of this study was to assess the viability, morphology, contamination, and antifungal susceptibility profile of fungi preserved in sterile water or paraffin oil compared to a periodic subculture.

Methods: This study was an experimental study using Aspergillus sp., Trichophyton mentagrophytes and Candida albicans. The fungi were preserved by a periodic subculture or in sterile water or in paraffin oil for six months. A comparison of viability, morphology, contamination, and antifungal susceptibility profile between methods were made before and after six months of study.

Results: The viability of all the fungi was maintained in both periodic subculture and preservation with paraffin oil. However, the T. mentagrophytes was not viable in preservation using sterile water. All methods could maintain the morphology without contamination. There were reductions in the inhibition zone against antifungal drug in preservation with sterile water and paraffin oil.

Conclusion: Preservation in paraffin oil is superior to preservation in sterile water in terms of maintaining fungal viability. This method is suitable for the use in a simple laboratory, albeit considerations should be given to the reduction of inhibition zone.


Fungi, paraffin oil, preservation, sterile water

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Smith D. Culture collections over the world. Int microbiol. 2003;95–100.

Machmud M. Teknik Penyimpanan dan Pemeliharaan Mikroba. Bul AgroBio. 2001;4(1):24–32.

Suciatmih, Rachmat. Pengujian Survival Jamur yang Dipreservasi Dalam Air dan Parafin Cair [ Survival Testing of Preserved Fungi in Water and Liquid-Paraffin ]. Ber Biol. 2005;7(5):241–8.

Ajello L, Grant VQ, Gutzke MA. Use of Mineral Oil in The Maintenance of Cultures of Fungi Pathogenic For Humans. Jama Netw. 1951;63(6):747–9.

Smith D, Onions AHS. A comparison of some preservation techniques for fungi. Trans Br Mycol Soc. 1983;81(3):535–40.

Buell CB, Weston WH. Application of the Mineral Oil Conservation Method to Maintaining Collections of Fungous Cultures. Am J Bot. 1947;34(10):555–61.

Surja SS, Adawiyah R, Houbraken J, Rozaliyani A, Sjam R, Yunihastuti E, et al. Talaromyces atroroseus in HIV and non-HIV patient: A first report from Indonesia. Med Mycol. 2019.

James C, Welsh C. Modern Microbiology Lab. 11th ed. Microbiology. England: Pearson Education; 2017.

Mcginnis MR, Padhye AA, Ajello L. Storage of Stock Cultures of Filamentous Fungi,Yeasts, and Some Aerobic Actinomycetes in Sterile Distilled Water. Appl Microbiol. 1974;28(2):218–22.

Rosana Y, Matsuzawa T, Gonoi T, Karuniawati A. Modified Slide Culture Method for Faster and Easier Identification of Dermatophytes. Microbiol Indones. 2014;8(3):135–9.

Haley LD, Callaway CS. Laboratory Methods in Medical Mycology. 4th ed. Georgia: U.S. Department of Health, Education and Welfare; 1978.

Surja SS, Wijaya M, Padmasutra L, Yolanda H, Joprang FS, Makimian R, et al. Atlas Parasitologi Kedokteran. 1st ed. Jakarta: Penerbit Universitas Katolik Indonesia Atma Jaya; 2019.

Agarwal RK, Gupta S, Mittal G, Khan F, Roy S, Agarwal A. Antifungal Susceptibility Testing of Dermatophytes by Agar Based Disk Diffusion Method. Int J Curr Microbiol Appl Sci. 2015;4(3):430–6.

Alexander BD, Procop GW, Dufresne P, Fuller J, Ghannoum MA, Hanson KE, et al. M60 Performance Standards for Antifungal Susceptibility Testing of Yeasts. 1st ed. Clinical and Laboratory Standards Institute. Clinical and Laboratory Standards Institute; 2017.

Alexander BD, Procop GW, Dufresne P, Fuller J, Ghannoum MA, Hanson KE, et al. M61 Performance Standards for Antifungal Susceptibility Testing of Filamentous Fungi. 1st ed. Clinical and Laboratory Standards Institute; 2017.

de Capriles CH, Mata S, Middelveen M. Preservation of fungi in water (Castellani): 20 years. Mycopathologia. 1989;106(2):73–9.

Karabıçak N, Karatuna O, Akyar I. Evaluation of the Viabilities and Stabilities of Pathogenic Mold and Yeast Species Using Three Different Preservation Methods Over a 12-Year Period Along with a Review of Published Reports. Mycopathologia. 2016;181(5–6):415–24.

Reggiori F, Klionsky DJ. Autophagic processes in yeast: Mechanism, machinery and regulation. Genetics. 2013;194(2):341–61.

Pollack JK, Harris SD, Marten MR. Autophagy in filamentous fungi. Fungal Genet Biol. 2009;46(1):1–8.

Hartsell SE. Microbiological process report. Maintenance of cultures under paraffin oil. Appl Microbiol. 1956;4(6):350–5.


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