Identification of Enteric Bacterial Pathogens in Beverages Sold By Hawkers around Jatinangor, Bandung
Abstract
Background: Water is essential for life and is considered as a medium for the propagation and dissemination of bacteria. Water-borne disease is one of the problems in Indonesia, therefore, this study was conducted to explore the presence of enteric bacterial pathogens in the beverages sold by hawkers.
Methods: A descriptive laboratory method was conducted in September 2015 Beverages were collected from hawkers that were randomly chosen around Jatinangor using sterile containers, and brought to the laboratory within 1 hour. The beverages were incubated on Mueller Hinton Broth, followed by culturing on MacConkey Agar. The organisms were identified using Gram Staining, Kliger Test, Motility-Indole-Urease test and Citrate test.
Results: Out of 30 samples collected, 2 of the samples were tested positive for enteric bacterial pathogen Salmonella paratyphi. Furthermore, Klebsiella pneumoniae (n=12), Enterobacterspp (n=10) Alcaligenes faecalis (n=3) and Pseudomonas spp. (n=3) were detected in the samples.
Conclusions: The Salmonella paratyphi as enteric bacterial pathogen found in the beverages sold by hawkers has potential to cause water-borne disease. Education to hawkers need to be enhanced.
Keywords
Full Text:
PDFReferences
Popkin BM, D’Anci KE, Rosenberg IH. Water, hydration, and health. Nutr Rev. 2010;68(8):439–58.
Gleeson C, Gray N. The coliform index and waterborne disease: problems of microbial drinking water assessment: London; CRC Press; 2002.
Matilainen A, Vepsäläinen M, Sillanpää M. Natural organic matter removal by coagulation during drinking water treatment: A review. Adv Colloid Interface Sci. 2010;159(2):189–97.
UNICEF, WHO. Progress on drinking-water and sanitation–2012 Update. New York: WHO Press; 2012.
Walters SP, Gannon VPJ, Field KG. Detection of Bacteroidales fecal indicators and the zoonotic pathogens E. coli O157:H7, salmonella, and campylobacter in river water. Environ Sci. Technol. 2007;41(6):1856–62.
Madec J-Y, Lazizzera C, Châtre P, Meunier D, Martin S, Lepage G, et al. Prevalence of fecal carriage of acquired expanded-spectrum cephalosporin resistance in Enterobacteriaceae strains from cattle in France. J Clin Microbiol. 2008;46(4):1566–7.
Mercado-Blanco J, Bakker PA. Interactions between plants and beneficial Pseudomonas spp.: exploiting bacterial traits for crop protection. Antonie Van Leeuwenhoek. 2007;92(4):367–89.
Akhtar S, Riaz M, Ismail T, Farooq U. Microbiological safety of street vended fresh fruit juices, drinks and conventional blends in Multan-Pakistan. Pak J Agri Sci. 2013;50(2):255–60.
Had S, Amer, Ustovi, Smajlovi J, Ahmetagi S. Distribution of nosocomial infections caused by Klebsiella pneumoniae ESBL strain. J Environ Occup Sci. 2012;1(3):141–6.
Zhu H, Hart CA, Sales D, Roberts NB. Bacterial killing in gastric juice – effect of pH and pepsin on Escherichia coli and Helicobacter pylori. J Med Microbiol. 2006;55(Pt9):1265–70.
Semenov AV, van Bruggen AHC, van Overbeek L, Termorshuizen AJ, Semenov AM. Influence of temperature fluctuations on Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in cow manure. FEMS Microbiol Ecol. 2007;60(3):419–28.
Alvarez-Ordóñez A, Begley M, Prieto M, Messens W, López M, Bernardo A, et al. Salmonella spp. survival strategies within the host gastrointestinal tract. Microbiology. 2011;157(Pt 12):3268–81.
Momtaz H, Dehkordi FS, Rahimi E, Asgarifar A. Detection of Escherichia coli, Salmonella species, and Vibrio cholerae in tap water and bottled drinking water in Isfahan, Iran. BMC Public Health. 2013;13:556.
DOI: https://doi.org/10.15850/amj.v6n1.1536
Article Metrics
Abstract view : 394 timesPDF - 353 times
This Journal indexed by
AMJ is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
View My Stats