Effect of High Fat and Cholesterol Diet on Total Blood Cholesterol Levels in Pregnant Wistar Rats

Desmawati Desmawati, Annisa Yuda Mahdiyah, Husnil Kadri

Abstract


Hypercholesterolemia during pregnancy is a physiological condition resulting from increased insulin resistance, lipoprotein synthesis, and lipolysis in adipose tissue, which mobilizes lipids as an energetic substrate for fetal growth. Consumption of foods high in fat and cholesterol may lead to an increase in total blood cholesterol levels during pregnancy due to saturated fat and cholesterol contents that will increases the synthesis of lipoproteins in the blood. The objectives of this study were to determine the effects of high fat and cholesterol diet on the total blood cholesterol levels in pregnant Wistar rats. This study was a true experimental research using a randomized post-test-only control group design  conducted from November 2020 to October 2021on fourteen female Wistar rats that were divided into control and intervention groups. Cow brain was provided as the high fat and cholesterol diet and after the rats gave birth, blood was drawn from the heart. The total blood serum cholesterol levels were assessed  using Micro Lab 300 with the CHOD-PAP method and the data were analyzed using an independent t-test. This study showed that the mean total blood cholesterol levels for the control and treatment groups were 80.43±18.512 mg/dL and  142.57±24.786 mg/dL, respectively, which reflected a significant differences in the mean total blood cholesterol level between the control and treatment groups (p-value <0.01). In conclusion, a high fat and cholesterol diet affects the total blood cholesterol level in pregnant Wistar rats.


Keywords


cholesterol; diet;high fat; hypercholesterolemia; pregnancy

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References


  1. Wahjuni S. Dislipidemia menyebabkan stress oksidatif ditandai oleh meningkatnya malondialdehid. Denpasar: Udayana University Press; 2015. p. 1–8.
  2. Arsana PM, Rosandi R, Manaf A, Budhiarta A, Permana H. Pedoman pengelolaan dislipidemia di Indonesia 2019. PB Perkeni. 2019;9.
  3. Akhfiya M, Syamsianah A. Kadar kolesterol total wanita menopause hiperkolesterolemia sesudah pemberian teh buah tin. J Unimus. 2018;7(2):49–53.
  4. Feitosa ACR, Barreto LT, Da Silva IM, Da Silva FF, Feitosa Filho GS. Impact of the use of different diagnostic criteria in the prevalence of dyslipidemia in pregnant women. Arq Bras Cardiol. 2017;109(1):30–8.
  5. Sabrida O, Hariadi H, Yantri E. Hubungan kadar LDL dan HDL serum ibu hamil aterm dengan berat lahir bayi. J Kesehat Andalas. 2014;3(3):289–96.
  6. Zhang R, Dong S, Ma WW, Cai XP, Le ZY, Xiao R, et al. Modulation of cholesterol transport by maternal hypercholesterolemia in Human fullterm placenta. PLoS One. 2017;12(2):1–11.
  7. Siringoringo HE, Yusrawati Y, Chundrayetti E. Perbedaan rerata kadar profil lipid pada preeklampsia dengan kehamilan normal pada etnik Minangkabau. J Kesehat Andalas. 2016;5(1):30–5.
  8. Maymunah AO, Kehinde O, Abidoye G, Oluwatosin A. Hypercholesterolaemia in pregnancy as a predictor of adverse pregnancy outcome. Afr Health Sci. 2014;14(4):967–73.
  9. Retnakaran R, Ye C, Hanley AJ, Connelly PW, Sermer M, Zinman B. Screening glucose challenge test in pregnancy can identify women with an adverse postpartum cardiovascular risk factor profile: implications for cardiovascular risk reduction. J Am Heart Assoc. 2019;8(21):1–10.
  10. WHO. Comprehensive implementation plan on maternal, infant and young child nutrition. Geneva: World Health Organization; 2014..
  11. Cerf ME, Herrera E. High fat diet administration during specific periods of pregnancy alters maternal fatty acid profiles in the near-term rat. Nutrients. 2016;8(1):1–14.
  12. Khan TJ, Ahmed YM, Zamzami MA, Mohamed SA, Khan I, Baothman OAS, et al. Effect of atorvastatin on the gut microbiota of high fat diet-induced hypercholesterolemic rats. Sci Rep. 2018;8(1):1–9.
  13. Li SW, Yu HR, Sheen JM, Tiao MM, Tain YL, Lin IC, et al. A maternal high-fat diet during pregnancy and lactation, in addition to a postnatal high-fat diet, leads to metabolic syndrome with spatial learning and memory deficits: beneficial effects of resveratrol. Oncotarget. 2017;8(67):111998–2013.
  14. Reis LB, de Mattos Paranhos Calderon I, Bolognani CV, Dias A, Dias A. Food consumption rated by quality index diet (IQD) in pregnant women with gestational diabetes mellitus. Diabetol Metab Syndr. 2015;7(S1):A67.
  15. Narasiang BR, Mayulu N, Kawengian S. Gambaran pola konsumsi makanan pada ibu hamil di kota Manado. J e-Biomedik. 2016;4(2):1-8.
  16. Xie CH, Zhang L, Zeng BH, Yuan J, Tang H, Wei H. Hypercholesterolemia in pregnant mice increases the susceptibility to atherosclerosis in adult life. Vascular. 2014;22(5):328–35.
  17. Bartels Ä, O’Donoghue K. Cholesterol in pregnancy: a review of knowns and unknowns. Obstet Med. 2011;4(4):147–51.
  18. Toleikyte I, Retterstol K, Leren TP, Iversen PO. Pregnancy outcomes in familial hypercholesterolemia. Am Heart Assosiation. 2011;124(15):1606–14.
  19. Hai Q, Smith JD. Acyl-Coenzyme A: Cholesterol Acyltransferase (ACAT) in cholesterol metabolism: From its discovery to clinical trials and the genomics era. Metabolites. 2021;11(8):543.
  20. Ngili, Y. Mekanisme reaksi dan metabolisme molekul. Bandung: Penerbit Rekayasa Sains; 2019. p. 431.
  21. Wang C, Kong L, Yang Y, Wei Y, Zhu W, Su R, et al. Recommended reference values for serum lipids during early and middle pregnancy: a retrospective study from China. Lipids in Health and Disease. 2018;17(1):1–16.
  22. Mathias JR, Dodd ME, Walters KB, Yoo SK, Erik A, Huttenlocher A. Rat maternal obesity and high fat diet program offspring metabolic syndrome. Am J Obstet Gynecol. 2014;211(3):1–13.
  23. Plows JF, Stanley JL, Baker PN, Reynolds CM, Vickers MH. The pathophysiology of gestational diabetes mellitus. Int J Mol Sci. 2018;19(11):1–21.
  24. Lestari WA. Resistensi insulin: Definisi, mekanisme dan pemeriksaan laboratoriumnya. Buku Ilmiah Clinical Pathology Update on Suramade. 2011;1:2011–1.
  25. Hidaka H, Tozuka M, Meyer B, Yamauchi K, Sugano M, Nakabayashi T, et al. Characterization of triglyceride rich lipoproteins with very light density by ultracentrifugation and agarose gel electrophoresis using triglyceride- and cholesterol-staining. Ann Clin Lab Sci. 2018;33(2):167–78.
  26. US Department of Agriculture. Food data central: Beef brain [Internet]. 2021 [cited 2021 March 15]. Available from: https://fdc.nal.usda.gov/.




DOI: https://doi.org/10.15395/mkb.v55n1.2651

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