Effect of High Fat Diet on Histopathological Appearance of Pregnant Wistar Rat’s Liver

Desmawati Desmawati, Ruhilmi Nisa, Nita Afriani

Abstract


Indonesia is facing increased consumption of calorie-dense foods, such as fast food, among its society. Changes in diet and physical activities towards unhealthy ones can result in an imbalanced lifestyle, leading to various consequences, including hyperlipidemia. Pregnant women experience significant increases in plasma concentrations of lipids, lipoproteins, and apolipoproteins. Excessive cholesterol levels in pregnant women will result in the formation of fat in the liver. The purpose of this study was to determine the effect of a high-fat diet to pregnant Wistar rats (Rattus norvegicus) on the histopathological features of the liver. A true experimental with post test only control group design was applied in this study, which was conducted from August 2020 to November 2021. Fourteen (14) pregnant female rats were divided into two groups. The first group of rats was fed with a high-fat diet, while the second group, which was the control group, was given a standard diet. The high-fat diet used was 3 mL/rat/day beef brain. After the rats delivered their litters, the rats’ livers were retrieved, then examined histopathologically under a microscope. This research was conducted from No fatty liver was identified in the control group or the treatment group. Hence, a high-fat diet does not affect the liver histopathological features in pregnant Wistar rats.

Keywords


high-fat diet; hepar cells;pregnant

Full Text:

PDF

References


  1. Listianasari Y, Dirgahayu P, Wasita B, Nuhriawangsa AMP. Efektifitas pemberian jus labu siam (sechium edule) terhadap profil lipid tikus (rattus novergicus) model hiperlipidemia. Penelit Gizi Makanan The J Nutr Food Res. 2017;40(1):35-43.
  2. Gitawati R, Widowati L, Suharyanto. Penggunaan Jamu pada Pasien Hiperlipidemia Berdasarkan Data Rekam Medik, di Beberapa Fasilitas Pelayanan Kesehatan di Indonesia. J Kefarmasian Indones. 2015;5(1):41–8.
  3. Dwinanda A, Afriani N, Hardisman H. Pengaruh jus seledri (apium graveolens l.) terhadap gambaran mikroskopis hepar tikus (rattus norvegicus) yang diinduksi diet hiperkolesterol. J Kesehat Andalas. 2019;8(1):68–75.
  4. Cunningham FG. Obstetri William. 23rd ed. (Setia R, ed.). Jakarta: Penerbit Buku Kedokteran EGC; 2009.
  5. R NB, Nelly M, Shirley K. Gambaran pola konsumsi makanan pada ibu hamil di kota Manado. J e-Biomedik. 2016;4(2):1–8.
  6. Heriansyah T. Pengaruh Berbagai Durasi pemberian diet tinggi lemak terhadap profil lipid tikus putih (rattus novergicus strain wistar) jantan. J Kedokt Syiah Kuala. 2013;13(3):144–50.
  7. Somba YR, Wongkar D, Ticoalu SHR, Bolang ASL. Gambaran histologik hati pada kelinci yang diinduksi lemak dengan pemberian ekstrak beras hitam. J e-Biomedik. 2016;4(2):2–6.
  8. Krisnansari D, Sulistyo H, Ati VRB. Efek propolis terhadap fungsi dan perlemakan hati (rattus norvegicus) model hiperkolesterolemia (the effect of propolis on liver function and fatty liver of hypercholesterolemic rat model). Penel Gizi Makan. 2014;37(1):77–85.
  9. Armanto RP. Deteksi dini perlemakan hati akut dalam kehamilan (acute fatty liver of pregnancy (AFLP)) dan penanganannya dengan diet rendah lemak. KELUWIH J Kesehat dan Kedokt. 2020;1(2):81–8.
  10. Pappachan JM, Babu S, Krishnan B, Ravindran NC. Non-alcoholic fatty liver disease: A clinical update. J Clin Transl Hepatol. 2017;5(4):384–93.
  11. Syafitri V, Arnelis A, Efrida E. Gambaran profil lipid pasien perlemakan hati non-alkoholik. J Kesehat Andalas. 2015;4(1):274–78.
  12. Yustiani NT, Arif M. Hubungan derajat perlemakan hati non-alkoholik dengan aktivitas aminotransferase serum. Indones J Clin Pathol Med Lab. 2018;16(1):26–8.
  13. Didit Damayanti, Pritasari N. Gizi dalam daur kehidupan. Jakarta: Kementrian Kesehatan Republik Indonesia; 2017.
  14. Priambudi PA, R Rindiani, Amareta DI. Efek pemberian buah pepino (solanum maricatum) terhadap perubahan kadar kolesterol total pada tikus hiperkolesterolemia. J Ilm Inov.. 2014;14(3):234–9.
  15. Shabah ZM, Dhanny DR. Hubungan indeks massa tubuh (IMT) dengan kadar kolesterol pada Mahasiswa Program Studi Kedokteran. Muhammadiyah J Nutr Food Sci. 2021;1(2):48–53.
  16. Hutami AT, Ratnawati, Wahyuningsih H. Hubungan antara indeks massa tubuh dengan kadar kolesterol (studi observasional analitik pada Mahasiswa Fakultas Kedokteran Universitas Islam Sultan Agung angkatan 2015). Pros Konf Ilm Mhs Unissula 2. 2019 October 18; Semarang, Indonesia: KIMU; 2019.
  17. Siregar FA, Makmur T. Metabolisme lipid dalam tubuh. J Inov Kesehat Masy. 2020;1(2):60–5.
  18. D’Adamo E, Castorani V, Nobili V. The liver in children with metabolic syndrome. Front Endocrinol (Lausanne). 2019;10:514.
  19. Arief M, Sulaeman A, Widodo J, Kasim S. Hubungan obesitas dan hipertrigliseridemia dengan risiko perlemakan hati pada pasien di Makassar. Indones J Clin Pharm. 2014;1(4):136–46.
  20. Mundiri NA, Maulida M, Tejasari M, Furqaani AR, Ekowati RAR. Pengaruh fraksi air buah lemon terhadap gambaran morfologi jaringan hati mencit tua yang diberi pakan tinggi lemak protective effect of water fraction of lemon on high-fat diet-induced liver injury in old mice. J Integr Kesehat Sains. 2019;1(22):49–53.




DOI: https://doi.org/10.15395/mkb.v54n3.2650

Article Metrics

Abstract view : 509 times
PDF - 500 times

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


 


Creative Commons License
MKB is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

 


View My Stats