Use of Herb Combination (Jamu Atoke) Before Mating to Improve Rat Pup Health

Andriyanto Andriyanto, Leliana Nugrahaning Widi, Hamdika Yendri, Kharisma Mardathilah, Diky Yuliansah, Firda Agustin, Aulia Andi Mustika, Wasmen Manalu

Abstract


Mortality and health disturbances in children often correlate with maternal health and fertility. Avocado, mung bean sprouts, and holy basil have been traditionally used to improve maternal health, before and during pregnancy. This study was aimed to assess the efficacy of herbal combination of avocado, mung bean sprouts, and holy basil (Jamu ATOKE) in optimizing reproductive health. Eighteen female Sprague Dawley rats (9–10 weeks old, BW: 180–250 g) were randomly divided into 3 treatment groups (n=6 rats for each group) of control, group I and II. ATOKE were added into rats drinking water and consumed for 30 days before pregnancy. After the rats were pregnant and gave birth, pup per parent ratio, pup mortality, and pup health performance (body weight gain, feed and drink consumption, motor activity, pup speed in finding light and feed, red blood cell (RBC) count, white blood cell (WBC) count and differential, SGPT, SGOT, blood urea nitrogen (BUN), creatinine, and proinflammatory mediators (TNF-α and IL-6) were analyzed. Pups born to in group I had a generally better health performance compared to the control group. There was a 100% mortality in the litter of group II due to the excessive aggressive behavior of the dams as a sign of toxicity. Administration of 2.5% Jamu ATOKE showed the best effects on the health and intelligence of the rat pup.


Keywords


Avocado, holy basil, Jamu ATOKE, maternal problems, mung bean sprouts

Full Text:

PDF

References


Andriyanto, Arif R, Winarto A, Soinbala LS, Achmadi B, Mustika AA. et al. Pemberian Pregnant Mare Serum Gonadotropin sebelum perkawinan dan jamu selama kebuntingan untuk memperbaiki performa anak domba. J Vet. 2014;16 (3):357–363.

Andriyanto, Amrozi, Rahminiwatt M, Boediono A, Manalu W. Optimum dose and time of pregnant mare serum gonadotropin injections in Kacang goats to increase endogenous secretion oestrogen and progesterone without superovulation response. Small Ruminant Res. 2017;149: 147–153.

Luke B, Gopal D, Cabral H, Stern JE, Diop H. Pregnancy, birth, and infant outcomes by maternal fertility status: the massachusetts outcomes study of assisted reproductive technology. Am J Obstet Gynecol. 2017;217(3):327.e1-327.e14.

Cao H, Wei X, Guo X, Chunying S, Luoa Y, Cui Y, et al. Screening high-risk clusters for developing congenital disabilities in mothers in Shanxi province, China: application of latent class cluster analysis. BMC Pregnancy Childbirth. 2015;15:343.

Baron-Cohen S, Tsompanidis S, Auyeung, Norgaard-Pedersen B, Hougaard DM, Abdallah M, et al. Foetal oestrogens and autism. Mol Psychiatry. 2020;25(11):2970–8.

Whitaker-Azmitia PM, Lobel M, Moyer A. Low maternal progesterone may contribute to both obstetrical complications and autism. Med Hypotheses.2014;82(3):313–8.

Gaskins AJ, Chavarro JE. Diet and fertility: a review. Am J Obstet Gynecol. 2018;218 (40):379–89.

Dreher ML, Davenport AJ. Hass avocado composition and potential health effects. Crit Rev Food Sci Nutr. 2013;53 (7):738–50.

Gioia FD, Petropoulos SA. Phytoestrogens, phytosteroids and saponins in vegetables: biosynthesis, functions, health effects and practical applications. Adv Food Nutr Res. 2019;90:351–421.

Bano N, Ahmed A, Tanveer M, Khan GM, Ansari MT. Pharmacological evaluation of Ocimum sanctum. J Bioequiv Availab. 2017;9 (3):387–92.

Martin CL, Siega-Riz AM, Sotres-Alvarez D, Robinson WR, Daniels JL, Perrin, et al. Maternal dietary patterns during pregnancy are associated with child growth in the first 3 years of life. J Nutr. 2016;146(11):2281–8.

Yeap SK, Beh KB, Ali NM, Yusof HM, Ho WY, Koh SP, et al. In vivo antistress and antioxidant effects of fermented and germinated mung bean. Biomed Res Int. 2014;2014:694842.

Ballard O, Morrow AL. Human milk composition: nutrients and bioactive factors. Pediatr Clin North Am. 2013;60(1):49–74.

Barha CK, Salvante KG, Jones MJ, Farré P, Blais J, Kobor MS. et al. Early post-conception maternal cortisol, children’s HPAA activity and DNA methylation profiles. J. Dev. Orig. Health. Dis. 2019;10 (1):73–87.

Alkhalf MI, Alansari WS, Ibrahim EA, Elhalwagy MEA. Anti-oxidant, anti-inflammatory and anti-cancer activities of avocado (Persea americana) fruit and seed extract. J King Saud Univ Sci. 2018;3:1358–62.

Tang D, Dong Y, Ren H, Li L, He C. A review of phytochemistry, metabolite changes, and medicinal uses pf the common food mung bean and its sprouts (Vigna radiata). hem Cent J. 2014;8(1):4.

Melo MF, Pereira DE, Moura RDL, Silva EBD, de Melo FA, Dias CD. et al. Maternal supplementation with avocado (Persea americana Mill.) pulp and oil alters reflex maturation, physical development, and offspring memory in rats. Front Neurosci. 2019;13:9.

Manikandaselvi S, David R, Aravind S, Ravikumar R, Thinagarbabu R, Nandhini S. Anti-anemic activity of sprouts of Vigna radiata L. in male albino rats. Int J Pharm Sci. 2015;7(11):263–7.

Das R, Raman RP, Saha H, Singh R. Effect of Ocimum sanctum Linn. (Tulsi) extract on the immunity and survival of Labeo rohita (Hamilton) infected with Aeromonas hydrophila. Aquac Res. 2015;46:1111–21.

Timilsina S, Karki S, Gautam A, Bhusal P, Paudel G, Sharma D. Correlation between maternal and umbilical cord blood in pregnant women of Pokhara Valley: a cross sectional study. BMC Pregnancy Childbirth. 2018;18:70.

Singh, A. Nutritional aspect of avocado: a review. Am Res Thoughts. 2015;1:2459–67.

Kang KS. Abnormality on liver function test. Pediatr Gastroenterol Hepatol Nutr. 2013;16(4):225–32.

Engelmann G, Hoffmann GF, Grulich-Henn, J, Teufel U. Alanine aminotransferase elevation in obese infants and children: a marker of early onset non alcoholic fatty liver disease. Hepat Mon. 2014;14 (4):14112.

Widhyari SD, Esfandiari A, Cahyono AD. Profil kreatinin dan nitrogen urea darah pada anak sapi Friesian Holstein yang disuplementasi Zn. Acta Vet Indones. 2015;3(2):45–50.

Abd El-Maksoud AM, Khairya SA, Sharadab HM, Abdallab MS, Ahmeda NF. Evaluation of pro-inflammatory cytokines in nutritionally stunted Egyptian children. Egyptian Pediatr Association Gazette. 2017;65(3):80–4.




DOI: https://doi.org/10.15395/mkb.v52n4.2629

Article Metrics

Abstract view : 395 times
PDF - 375 times

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


 


Creative Commons License
MKB is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

 


View My Stats