Gestational Monosodium Glutamate Exposure Effects on Anogenital Distance of Male Rat Pups
Abstract
High-dose Monosodium Glutamate (MSG) expo sure increases the estrogen level in pregnant rats. However, there are limited data available on whether the MSG-related maternal hormonal effects can affect male litters' genitalia phenotype. This study aimed to analyze the impact of MSG on estrogen level in pregnant rats and anogenital distance in male pups. Experiment for this study was performed at the animal facility of Biomedical Laboratory at the Faculty of Medicine, Universitas Andalas, from April 2019 to February 2020. Pregnant Wistar rats were given MSG orally at 2 and 4 mg/g body weight (BW) for 20 days. On day 21, pregnant rats were sacrificed and blood was drawn intracardially. Estradiol serum level was measured by ELISA. Male pups were counted, and the anogenital distance (AGD) was measured. Maternal serum estradiol levels were statistically analyzed by One-Way ANOVA and the AGD of male litters were analyzed by the Kruskal-Wallis test. Results showed that perinatal MSG exposure increased the estradiol level (26.3±4.5 pg/ml; 37.5±6.7 pg/ml; 62.1±8.2 pg/ml in control, 2 mg/g BW, and 4 mg/g BW group, respectively [mean±SD; p=<0.001]) and decreased the AGD (4 mm; 3 mm; 1.5 mm in control, 2 mg/g BW, and 4 mg/gBW group, respectively [median; p=<0.01]) in a dose-dependent manner. Thus, MSG exposure during pregnancy is a risk factor for male rat feminization.
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Kurtanty D, Faqih DM, Upa NP. Review monosodium glutamate. Jakarta: Ikatan Dokter Indonesia; 2018
Oladipo IC, Adebayo EA, Kuye OM. Effect of monosodium glutamate in ovaries of female Sprague Dawley rats. International Journal of Microbiology and Applied Science. 2015;4(5):737-745.
Iamsaard S, Sukhorum W, Samrid R, Yimde J, Kanla P, Hipkaeo W, et al. The sensitivity of male reproductive organ to monosodium glutamate. Medica Academica. 2014;43(1): 3-9.
Okoye CN, Ochiogu LS, Onah CE. The effect of monosodium glutamate administration of the reproduction and serum biochemistry of adult male rabbits. Veterinary Medicine. 2016;61(3):141-147.
Mondal M, Sarkar K, Nath PN, Paul G. Monosodium glutamate suppresses the female reproductive function by impairing the function of ovary and uterus in the rat. Environmental Toxicology. 2017;1-11. Doi: 10.1002/tox.22508
Eweka AO, Eweka A, Om'iniabohs FAE. Histological studies of the effect of monosodium glutamate of the fallopian tubes of adult female Wistar rats. North American Journal of Medical Sciences. 2010;2(3):146-149.
Hsieh MH, Breyer BN, Eisenberg, ML, Baskin LS. Associations among hypospadias cryptorchidism, anogenital distance, and endocrine disruption. Current Urology Report. 2008;9:137-142.
Walker VR, Jefferson WN, Couse JF, Korach KS. Estrogen receptor-α mediates diethylstilbestrol-induced feminization of the seminal vesicle male mice. Environmental Health Perspectives. 2012;120(4):560-565. Doi.org /10.1289 /ehp. 1103978
Zia MS, Qamar K, Hanif R, Khalil M. Effect of monosodium glutamate on the serum estrogen and progesterone levels in female rat and prevention of this effect with diltiazem. Journal of Ayub Medical College Abbottabad. 2014;26(1):18-20.
Nwajei JC, Onuoha SC, Essien EB. Effect of oral administration of selected food seasonings consumed in Nigeria on some sex hormones of Wistar albino rats. Journal of Biotechnology and Biochemistry. 2015;1(5):15-21.
He K, Du S, Xun P, Sharma S, Wang H, Zhai F et al. Consumption of monosodium glutamate in relation to incidence of overweight in Chinese adult: China health and nutrition survey (CHNS). American Society for Nutrition. 2011;93:1328-1336
Onaolapo OJ, Onaolapo AY, Akanmu MA, Gbola O. Evidence of alterations in brain structure and antioxidant status following low dose monosodium glutamate ingestion. Pathophysiology. 2016;23:147-156.
Afifi MM, Abbas AM. Monosodium glutamate versus diet-induced obesity in pregnant rats and their offspring. Acta Physiologica Hungarica. 2011;98(2):177-188. DOI: 10.1556/Physiol.98.2011.2.9.
Ogbuagu EO, Airadion AI, Okoroukwu VN, Ogbuagu U, Ekonjoku JA. Effect of Monosodium glutamate on body weight and alanine aminotransferase activity in wistar rats. International Research Journal of Gastroenterology and Hepatology. 2019;2(2):1-8.
Gaspar RS, Benevides ROA, Fontelles JLL, Vale CC, França LM, Barros PTS et al. Reproductive alterations in hyperinsulinemic but normoandrogenic MSG obese female rats. Journal of Endocrinology. 2016;229:61-72.
Obochi GO, Malu SP, Abang MO, Alozie Y, Iyam MA. Effect of garlic on monosodium glutamate induced fibroid in wistar rats. Pakistan Journal of Nutrition. 2009;8(7):979-976
Mitchell RT, Mungall W, McKinnell C, Sharpe RM, Cruickshanks L, Milne L, et al. Anogenital distance plasticity in adulthood: Implication for its use as a biomarker of fetal androgen action. Endocrinology. 2015;156(1):24-31. Doi: 10.1210/en.2014-1534.
Thankamony A, Lek N, Carroll D, Williams M, Dunger DB, Acerini CL, Ong KK, Hughes IA. Anogenital distance and penile length in infants with hypospadias or cryptorchidism: comparison with normative data. Environ Health Perspect. 2014; 122(2):207–211. Doi.org/10.1289/ehp.13071 78.
Gallavan RH, Holson JF, Stump DG, Knapp JF, Reynolds VL. Interpreting the toxicologic significance of alterations in anogenital distance: potential for confounding effect of progeny body weights. Reproductive Toxicology.1999;13(5):383-390.
DOI: https://doi.org/10.15395/mkb.v53n2.2302
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