Effect of Mangkokan (Polyscias scutellaria) Leaf Extract on Blood Sugar Levels in Alloxan-Induced Male White Rats

Sri Lestari Ramadhani Nasution, Awanis Awanis, Stiven Elsafarindo

Abstract


Type 2 diabetes is a metabolic disorder caused by insulin resistance and is associated with oxidative stress. In Indonesia, the mean prevalence of diabetes ranges from 1.4% to 1.6%; however, some areas have a much higher diabetes prevalence such as Pekajangan (2.3%) and in Manado (6%). The 2013 Indonesian Basic Health Research (IBHR) stated that the number of people with DM in Indonesia has reached an alarming rate. This study aimed to determine the antidiabetic effect of Polyscias scutellaria on alloxan-induced male Wistar. This was an experimental study conducted in July 2020 in the Faculty of Medicine, Prima Indonesia University. This study used 25 rats that were grouped into 5 treatment groups: control group (Na-CMC), standard (Metformin), and 3 extract groups with different doses (125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW). Mangkokan leaf extract was obtained through the maceration method. All ratswere induced intraperitoneally using alloxan monohydrate 10% at a dose of 175 mg/kg BW. The parameters used in this study were fasting blood glucose level before induction, after induction, and after treatment and body weight before treatment. It was observed that there was a significant change in blood glucose level between the extract groups. The blood sugar level in the 125 mg/kg BW group was 495.00 mg/dl while in the 250 mg/kg BW and 500 mg/kg BW, the blood glucose levels were 317.00 mg/dl and 126.00 mg/dl, respectively, with the 500 mg/Kg BW dose as the most effective dose (P-value = 0.001). Thus, mangkokan leaves have the potential to reduce blood glucose level but are not as good as the standard group.


Keywords


Blood glucose level, mangkokan leaves, metformin, pancreas

Full Text:

PDF

References


Purnamasari D. Diagnosis dan klasifikasi diabetes melitus. In: Setiati S, Alwi I, Sudoyo AW, Simadibrata M, Setiyohadi B, Syam AF, editor. Buku ajar ilmu penyakit dalam. 6th ed. Jakarta: Interna Publishing; 2014. p. 2569–98.

Ndraha S. Diabetes melitus tipe 2 dan tatalaksana terkini. Medicinus. 2014;27(2): 9–16.

IDF. Diabetes Atlas Ninth edition 2019. Individual, social and economic impacts. Brussels: IDF Advocacy Guide; 2019.

Soelistijo SA. Konsensus pengendalian dan pencegahan diabetes melitus tipe 2 di Indonesia. Jakarta: PB PERKENI; 2015.

Tarigan THE, Yunir E, Subekti I. Profile and analysis of diabetes chronic complications in outpatient diabetes clinic of Cipto Mangunkusumo Hospital, Jakarta. Med J Indones. 2015;24(3):156–62.

Kementerian Kesehatan Republik Indonesia. Hasil utama riset kesehatan dasar. Jakarta: Kementerian Kesehatan RI; 2019; 1–200. https://doi.org/10.1088/1751-8113/44/8/085201%.

Forouhi NG, Wareham NJ. Epidemiology of diabetes. Medicine (Abingdon). 2014;42(12): 698–702.

Isnaini N, Ratnasari R. Faktor risiko mempengaruhi kejadian diabetes mellitus tipe dua. J Kebidanan dan Keperawatan Aisyiyah. 2018;14(1):59–68.

Oguntibeju OO. Type 2 diabetes mellitus, oxidative stress and inflammation: examining the links. Int J Physiol Pathophysiol Pharmacol. 2019;11(3):45–63.

Amani ZA, Mustarichie R. Review artikel : aktivitas antihiperglikemia beberapa tanaman di Indonesia. Farmaka. 2018;16(1): 127–32.

Jubaidah S, Indriani R, Sa’adah H, Wijaya H. Formulasi dan uji pertumbuhan rambut kelinci dari sediaan hair tonic kombinasi ekstrak daun seledri (apium graveolens linn) dan daun mangkokan (polyscias scutellaria (burm.f.) fosberg). J Ilm Manuntung. 2018; 4(1):8–14.

Eden WT, Buanasari, Shihabuddin, Badahdah NK. Aktivitas antioksidan ekstrak metanol daun mangkokan (polyscias scutellaria (burn.f.)fosberg). Media Farm Indones. 2016;11(2):1126–35.

Rosa D, Halim Y, Kam N, Sugata M, Samantha A. Antibacterial activity of polyscias scutellaria fosberg against acinetobacter sp. Asian J Pharm Clin Res. 2019;12(1):516–9.

Widowati W, Wargasetia TL, Afifah E, Mozef T, Kusuma HSW, Nufus H, et al. Antioxidant and antidiabetic potential of Curcuma longa and its compounds. Asian J Agric Biol. 2018; 6(2):149–61.

Njagi E N Mwaniki MJM, Njagi J Murugi NMP. In vivo anti-diabetic effects of aqueous leaf extracts of rhoicissus tridentata in alloxan induced diabetic mice. J Dev Drugs. 2015;04(03):1–5.

Zubaidah E, Ifadah RA, Kalsum U, Lyrawati D, Putri WDR, Srianta I, et al. Anti-diabetes activity of Kombucha prepared from different snake fruit cultivars. Nutr Food Sci. 2019;49(2):333–43.

Revina M, Yuliani R, Putri M, Hulu W, Sinaga A, Nasution SLR. Efektivitas ekstrak daun mangkokan terhadap penyembuhan luka bakar pada tikus. Sci J. 2018;7(2):166–72.

Nasution SLR, Revina M, Yuliani R, Nasution SW. The Effectiveness comparison of aloe vera and nothopanax schutellarium exract toward thermal burn on white rat (rattus norvegicus). J Educ Heal Sport. 2019;9(4):580–90.

Ighodaro OM, Adeosun AM, Akinloye OA. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina (Kaunas). 2017;53(6):365–74.

Vijayaraj R, Kumaran NS, Swarnakala. In vivo and in vitro models for biological screening of anti-diabetic drugs. Int J Pharm Sci. 2019;9(2):294–8.




DOI: https://doi.org/10.15395/mkb.v53n3.2223

Article Metrics

Abstract view : 664 times
PDF - 512 times

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


 


Creative Commons License
MKB is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

 


View My Stats