Anti-Inflammatory Effect of Centella asiatica (L.) Extract by Decreasing TNF-α Serum Levels in Rat Model of Traumatic Brain Injury
Abstract
Centella asiatica (L.) has many active ingredients with many important roles, including as antioxidant, anti-inflamation and neuroprotectant. Centella asiatica (L.) can reduce inflammatory reactions by inhibiting the activity of TNF-α. Thus, Centella asiatica (L.) is a potential alternative therapy for traumatic brain injury by reducing inflammation via TNF-α expression modulation. This study aimed to determine the effect of Centella asiatica (L.) on serum TNF-α levels in rat model of traumatic brain injury. This study was conducted during the period of July 3-17, 2020 at the LPPT Unit IV, Gajah Mada University. This was a true experimental with post-test only control group study on 35 male wistar rats as the experimental animals. The rats were divided into 5 groups: P1, P2, and traumatic brain injury groups that received Centella asiatica (L.) treatement at 150, 300, and 600mg/kgBW/d doses, respectively. Blood samples were collected after the experimental animals were terminated to assess serum TNF-α levels. Mean TNF-α levels were 60,980±4,057, 76,931±0,698, P3=75,889±0,948, P4=75,868±1,163, and 74,508±1,126 for P1, P2, P3, P4, and P5, respectively. The Kruskal Wallis test results showed a statistically different between groups (p = 0.005). This study shows that Centella asiatica (L.) can decrease serum TNF-α level in rat model of traumatic brain injury.
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Freire MAM. Pathophysiology of neurodegeneration following traumatic brain injury. West Indian Med Journal. 2012;61(7);p. 751-755.
Kementerian Kesehatan RI Badan Penelitian dan Pengembangan. Hasil Utama Riset Kesehatan Dasar. Jakarta: Badan penelitian dan Pengembangan Kesehatan Kementrian Kesehatan Republik Indonesia; 2018;. p. 1–200.
Faul M, Xu L, Wald MM and Coronado VG. Traumatic Brain Injury in the United States: Emergency Department Visits, Hospitalizations and Deaths 2002–2006. Atlanta: Centers for Disease Control and Prevention, National Center for Injury Prevention and Control; 2010; p. 1-74.
Badjatia N, Carney N, Crocco TJ, Fallat ME, Hennes HMA, Jagoda AS, et al. Guidelines for prehospital management of traumatic brain injury 2nd edition. Prehospital Emergency Care. 2008;12(1):1–522.
Prasetyo BH, Handono K, Dalhar M. Pengaruh Pemberian Ekstrak Propolis terhadap Ekspresi TLR4 dan Apoptosis pada Jaringan Otak Tikus Model Traumatic Brain Injury (TBI). Jurnal Kedokteran Brawijaya. 2017;29(4):287–292.
Andrzejowski J, Fraser J. Cottrell and Young’s Neuroanaesthesia. British Journal of Anaesthesia. 2010;105(5):704.
Sari LM. Apoptosis: Mekanisme Molekuler Kematian Sel. Cakradonya Dental Journal. 2018;10(2):65–70.
Jazmi AF, Alfiantya PF, Nurarifah SAH, Purmitasari EA, Vitania LA, Riawan W. Spade Leaf Extract Phytosome Modulates Krox-20, Neuregulinـ1, Phospholipids, and Cognitive Function of Traumatic Brain Injury Model in Rats. Indonesian Journal of Cancer Chemoprevention. 2017;6(3):105-110.
Khotimah H, Riawan W, and Kalsum, U. Efek Neuroprotektif Ekstrak Pegagan (Centella asiatica) terhadap BDNF (Brain-Derived Neurotropic Factor), TNFaR, NFkB dan Apoptosis Pada Kultur Sel Syaraf yang Diinduksi LPS. Laporan Hibah Penelitian Strategis Nasional. Direktorat Jenderal Pendidikan Tinggi, Departemen Pendidikan Nasional; 2009; p.1-9.
Sharma S andThakur SC. Centella asiatica extract protects against both inflammation and oxidative stress in collagen-induced arthritis rat model. Journal of Natural Science, Biology and Medicine. 2011;2(3):117.
Chen H, Hua XM, Ze BC, Wang B, and Wei L. The anti-inflammatory effects of asiatic acid in lipopolysaccharide-stimulated human corneal epithelial cells. International Journal of Ophthalmology. 2017;10(2):179–185.
Wan J, Gong X, Jiang R, Zhang Z, and Zhang L. Antipyretic and anti-inflammatory effects of asiaticoside in lipopolysaccharide-treated rat through up-regulation of heme oxygenase-1. Phytotherapy Research. 2013;27(8):1136–1142.
George M, Joseph L, and Ramaswamy. Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella asiatica extracts. African Journal of Traditional, Complementary and Alternative Medicine. 2009;6(4):554–559.
Chong NJ and Aziz Z. A systematic review on the chemical constituents of Centella asiatica. Research Journal of Pharmaceutical, Bioogical and Chemical Science. 2011;2(3):445–459.
Maleki SJ, Crespo JF, and Cabanillas B. Anti-inflammatory effects of flavonoids. Food Chemistry. 2019;299(2019):1-11.
Rochmah MA, Harini IM, Septyaningtrias DE, Sari DCR, and Susilowati R. Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic Stress. Biomed Research International. 2019;1-7.
Lumbessy M, Abidjulu J, and Paendong JJ. Uji Total Flavonoid pada Beberapa Tanaman Obat Tradisonal di Desa Waitina Kecamatan Mangoli Timur Kabupaten Kepulauan Sula Provinsi Maluku Utara. Jurnal MIPA. 2013; 2(1): 50-55.
DOI: https://doi.org/10.15395/mkb.v53n2.2222
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