Comparison of Antioxidant and Antiaging Activities Between Dragon Fruit (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose) Rind Extract and Kaempferol
Abstract
Aging involves progressive physiological changes of the body, including skin dysfunction and death which can be accelerated by the presence of free radicals. Compounds with potential antioxidants and antiaging are contained in various fruits, including dragon fruit (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose). This study aimed to compare the antioxidant and antiaging activities of dragon fruit rind extract (DFRE) and Kaempferol. The antioxidant and antiaging activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenger and inhibitory activity assay of tyrosinase, respectively and was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama, from August to November 2018. Data acquired were then analyzed using ANOVA, followed by Post Hoc Test using Turkey HSD test with a confidence level of 95% (α=0.05). Phytochemical assay was also conducted to determine the content of active ingredients in thedragon fruit rind extract. Phytochemical test result showed that DFRE contained flavonoids, tannins, phenols, triterpenoids, and alkaloids compounds. Both DPPH scavenging and tyrosine inhibition assay indicated that Kaempferol compound also had antioxidant and antiaging activities. The IC50 values of DFRE and Kaempferol antioxidant activities were 164.98 μg/mL and 83.30 μg/mL, respectively, while the IC50 values of tyrosinase inhibition activity of DFRE and Kaemferol were 88.46 μg/mL and 59.34 μg/mL, respectively. DFRE had antioxidant activities and tyrosinase inhibition activities thus can be used as an alternative antiaging and antioxidant agents.
Perbandingan Aktivitas Antioksidan dengan Antitirosinase Ekstrak Etanol Kulit Buah Naga (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose)
Aging atau penuaan adalah proses kompleks yang ditandai dengan penurunan progresif fungsi fisiologis tubuh, termasuk kulit yang diikuti oleh disfungsi dan kematian kulit. Faktor-faktor yang dapat mempercepat proses penuaan dini di antaranya radikal bebas dan gaya hidup yang tidak sehat. Buah-buahan banyak mengandung senyawa yang berpotensi sebagai antioksidan dan antiaging, salah satu di antaranya adalah buah naga. Penelitian ini bertujuan mengetahui potensi antioksidan dan antiaging ekstrak kulit buah naga (EKBN) yang dibandingkan dengan senyawa kaempferol (SK) sebagai pembanding. Penelitian yang dilakukan meliputi uji fitokimia untuk mengetahui kandungan bahan aktif pada kulit buah naga, uji pemerangkapan DPPH, dan uji penghambatan tirosinase dan dilakukan di Pusat Penelitian Biomolekul dan Biomedis, Aretha Medika Utama, dari Agustus hingga November 2018. Data lalu dianalisis menggunakan analisis varians (ANOVA) satu arah (one way) Hasil penelitian uji fitokimia menunjukkan EKBN mengandung golongan senyawa, flavonoid, tanin, fenol, triterpenoid, dan alkaloid serta tidak mengandung golongan senyawa saponin, steroid, dan terpenoid. Senyawa kaempferol memiliki aktivitas antioksidan melalui pemerangkapan DPPH dan aktivitas antiaging melalui penghambatan tirosinase yang lebih baik dibanding dengan EKBN. Aktivitas antioksidan dilihat dari nilai IC50 EKBN sebesar 164.98 μg/ml dan SK sebesar 83.30 μg/mL. Aktivitas penghambatan tirosinase memiliki nilai IC50 EKBN masing-masing sebesar 88.46 μg/mL dan IC50 SK sebesar 59.34 μg/mL. EKBN memiliki aktivitas antioksidan dan penghambatan tirosinase sehigga dapat dimanfaatkan sebagai alternatif bahan antiaging dan antioksidan.
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Widowati W, Janeva BW, Nadya S, Amalia A, Arumwardana S, Kusuma HSW, Arinta Y. Antioxidant and antiaging activities of jasminum sambac extract, and its compounds. JRPS. 2018;7(3);270–85.
Manihuruk FM, Suryati T, Arief II. Effectiveness of the red dragon fruit (hylocereus polyrhizus) peel extract as the colorant, antioxidant, and antimicrobial on beef sausage. Media Peternakan. 2017; 40(1):47–54.
Li S, Tan H, Wang N, Zhang Z, Lao L, Feng Y. The role of oxidative stress and antioxidants in liver disease. Int J Mol Sci. 2015;16(11):26087–124.
Romdonah, F, Kusumo E, Supartono S. Identifikasi betasianin dan uji antioksidan ekstrak kulit buah naga merah (hylocereus polyrhiyus. Indonesian J Chem Sci. 2017; 6(1):1–4.
Soong Y, Barlow PJ. Antioxidant activity and phenolic content of selected fruit seeds. Food Chemistry. 2004;88(3):411–7.
Widowati W, Fauziah N, Heddy H, Afni M, Afifah E, Kusuma HSW, et al. Antioxidant and antiaging assays of Oryza sativa extracts, vanilin and coumaric acid. J Nat Remed. 2016;16:88–99.
Widowati W, Wijaya L, Wargasetia T, Bachtiar I, Yellianty Y, Laksmitawati D. Antioxidant, anticancer, and apoptosisinducing effects of piper extracts in hela cells. J Exp Integr Med. 2013; 3(3):225–30.
Widowati W, Mozef T, Risdian C, Yellianty Y. Anticancer and free radical scavenging potency of Catharanthus roseus, Dendrophthoe petandra, Piper betle, and Curcuma mangga extracts in breast cancer cell lines. Oxidants Antioxid Med Sci. 2013; 2(2):137–42.
Widowati W, Rani AP, Hamzah RA, Arumwardana S, Afifah E, Kusuma HSW, et al. Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Nat Prod Sci. 2017;23:192–200
Fais A, Corda M, Era B, Fadda MB, Matos MJ, Quezada QE, et al. Tyrosinase inhibitor activity of coumarin-resveratrol hybrids. Molecules. 2009;14(7):2514–20.
Tu PTB, Tawata S. Antioxidant, antiaging, and anti-melanogenic properties of the essential oil from two varieties of Alpinia zerumbet. Molecules. 2015; 20(9):16723–40.
Rusmana D, Wahyudianingsih R, Elisabeth M, Balqis B, Maesaroh M, Widowati W. Antioxidant activity of Phyllanthus niruri extract, rutin and quercetin. Indones Biomed J. 2017;9(2):84–90.
Nurliyana R, Zahir IS, Suleiman KM, Aisyah MR, Rahim KK. Antioxidant study of pulps and peels of dragon fruits: a comparative study. Int Food Research J. 2010;17(2):367–65.
Amalia S, Wahdaningsih S, Untari EK. Antibacterial activity testing of n-hexane fraction of red dragon (Hylocereus polyrhizus Britton & Rose) fruit peel on Staphylococcus aureus ATCC 25923. Trad. Med J. 2015;19(2):89–94.
Widowati W, Ratnawati H, Rusdi U, Winarno W, Imanuel V. Phytochemical assay and antiplatelet activity of fractions of velvet been seeds (Mucuna pruriens). HAYATI J Biosci. 2010;17(2):85–90.
Lourith N, Kanlayavattanakul M. Antioxidant and stability of dragon fruit peel colour. Agro Food Industry Hi Tech. 2013;24(3):56–8.
Cai Y, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem. 2003;51(8):2288–94.
Vijayakumar R, Gani SSA, Uswatun Hasanah Zaidan UH, Halmi MIE. Optimization of the antioxidant potentials of red pitaya peels and its in vitro skin whitening properties. Appl Sci. 2018;8(9):1516.
Casañola-Martin GM, Marrero-Ponce Y, Hassan MT. Dragon method for finding novel tyrosinase inhibitors: Biosilico identification and experimental in vitro assays. Eur J Med. Chem. 2007;42(11-12):1370–81.
Kamkaen N, Mulsri N, Treesak C. Screening of some tropical vegetables for anti-tyrosinase activity. Thai Pharm. Health Sci. J. 2007;2(1): 15–19.
DOI: https://doi.org/10.15395/mkb.v51n3.1715
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