Old Mice Epididymal Sperm Quality After Short Term Gavage of Cogon Grass Root Ethanol Extract

Alkaustariyah Lubis, Rini Widyastuti, Sondi Robianto, Madeleine Priscilla, Mas Rizky A. A. Syamsunarno


Cogon grass (Imperata cylindrica) has been known as traditional herb because of its antioxidant activity. The oxidant level in the body increases linearly with age. Antioxidant works by decreasing the vulnerability of mammalian spermatozoa towards free radical attack. This study aimed to determine whether the active compound of Imperata cyclindrica root extract improved or damaged  sperm function in old mice. This was a complete randomized study performed in the Mouse Animal Laboratory Faculty of Medicine, Universitas Padjadjaran during the period of August to December 2017. This study was carried out by oral administration of three different doses i.e 115, 230, and 345 mg/kg body weight of cogon grass root ethanol extract into 24- weeks-old DDY strain mice for 14 days. Results showed no significant difference in body weight  and testicular index before and after treatment (p<0.05). Interestingly, sperm concentration decreased significantly in 345 mg/kgBW group when compared  to control (2.03x106 and 6.43x106 respectively, p<0.05). On the other hand, sperm motility and sperm with normal morphology increased following dose pattern, although not significantly, when compared to control (p<0.05). Hence, cogon grass root ethanol extract cannot maintain optimum epididymal sperm quality in old mice. 

Key words: Aging, Imperata cylindrica, sperm quality


Kualitas Sperma Epididimis Mencit Tua setelah Pemberian Ekstrak Akar Alang-Alang secara Oral Jangka Pendek

Alang-alang (Imperata cylindrica) dikenal sebagai obat tradisional karena efek antioksidan yang dimiliki. Kadar oksidan pada tubuh meningkat seiring pertambahan usia. Antioksidan bekerja dengan cara mengurangi kerentanan spermatozoa terhadap radikal bebas. Penelitian ini bertujuan untuk menentukan apakah komponen aktif ekstrak akar alang-alang dapat memperbaiki atau merusak fungsi sperma pada mencit tua. Penelitian ini dilakukan di Laboratorium Hewan Fakultas Kedokteran Universitas Padjadjaran periode Agustus hingga Desember 2017. Eksperimen dibagi menjadi satu grup kontrol dan tiga grup perlakuan dengan dosis 115, 230 dan 345 mg/kgBB selama 14 hari. Hasil menunjukkan tidak ada perbedaan signifikan pada berat badan dan index testis sebelum dan sesudah perlakuan. Menariknya, jumlah sperma menurun dengan signifikan pada kosentrasi tertinggi 345 mg/kgBB dibanding kontrol, yaitu 2,03x106 dan 6,43x106 (p<0.05). Selain itu, terjadi peningkatan motilitas sperma dan morfologi normal secara gradual hingga dosis tertinggi dibanding dengan kontrol walaupun tidak signifikan. Berdasar atas hasil yang didapat, ekstrak akar alang-alang dapat menjaga kualitas sperma pada mencit tua walaupun tidak optimal.

Kata kunci: Imperata cylindrica, kualitas sperma, penuaan



Aging, Imperata cylindrica, sperm quality

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Tremellen K. Oxidative stress and male infertility-aclinical perspective. Hum Reprod Update. 2008;14:243–58.

Padma R, Parvathy NG, Renjith V, Kalpate PR. Quantitave estimation of tannins, phenols and antioxidant activity of methanolic extract of Imperata cylindrica. Int J Res Pharm Sci 2013;4(1):73–7.

Zhou X, Wang J, Jiang B, Shang J, Zhao C. A study of extraction process and in vitro antioxidant activity of total phenols from rhizoma imperatae. Afr J Tradit Complement Altern Med. 2013;10(4):175–8.

Rong-hua L, Li-na F, Lan-ying C, Gang R, Shi-sheng C, Zhuo C. Chemical constituents and pharmacological study progress of imperata cylindrical rhizomes. J Jiangxi University Traditional Chinese Med. 2010;22(4):80–3.

Yoshida S, Kyoko H, Shigeru O, Hirohisa T, Ken T, Takamichi I. Effects of fetal exposure to carbon nanoparticles on reproductive function in male offspring. Fertil Steril. 2010;93(5):1695–9.

WHO, Department of Reproductive Health and Research. WHO laboratory manual for the examination and processing of human semen; 2010.

Zitzmann M. Effect of age on male fertility. Best Pract Res Clin Endocrinol Metab. 2013;27(4):617–28.

Stone BA, Alex A, Werlin LB, Marrs RP. Age thresholds for changes in semen parameters in men. Fertil Steril. 2013;100:952–8.

Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010;16(3):231–45.

Shiva M, Gautam AK, Verma Y, Shivgotra V, Doshi H, Kumar S. Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clin Biochem. 2011;44(4):319–24.

Kao SH, Chao HT, Chen HW, Hwang TI, Liao TL, Wei YH. Increase of oxidative stress in human sperm with lower motility. Fertil Steril. 2008;89:1183–90.

Wang Y, Chen F, Ye L, Zirkin B, Chen H. Steroidogenesis in Leydig cells: effect of aging and environtmental factors. Reproduction. 2017;154(4):R111–22.

Krisnamurthy H, Sairam MR. Mammalian endocrinology and male reproduction biology. Florida: CRC Press; 2015.

Widyastuti R, Wira DW, Gita T, Fauziah N, Syamsunarno MR. Efek pemberian ekstrak etanol akar alang-alang (imperata cylindrica) terhadap perubahan berat badan dan organ reproduksi mencit jantan. Jurnal Ilmu Ternak. 2017;17(1):47–51.

Zada A, Dewanto JB, Dahlan A, Dhianawaty, D, Syamsunarno MRAA, Mukarromah GR, et al. Root extract of Imperatacylindrica L. improves serum nitric oxide levels in diabetic mice. Unity in Diversity and the Standardisation of Clinical Pharmacy Services: Proceedings of the 17th Asian Conference on Clinical Pharmacy (ACCP 2017), July 28-30, 2017, Yogyakarta, Indonesia: CRC Press; 2017.

Ola-Mudathir KF, Suru SM, Fafunso MA, Obioha UE, Faremi TY. Protective roles of onion and garlic extracts on cadmium-induced changes in sperm characteristics and testicular oxidative damage in rats. Food Chem Toxicol. 2008;46(12):3604–11.

Tu¨rk G, Sönmez M, Aydin M, Yüce A, Gür S, Yüksel M, et al. Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin Nutr. 2008;27(2):289–96.

Chemes HE, Alvarez Sedo C. Tales of the tail and sperm head acheschanging concepts on the prognostic significance of sperm pathologies affecting the head, neck, and tail. Asian J Androl. 2012;14(1):14–23.

Kumbar MN, Kamble RR, Kamble AA, Salian SR, Kumari S, Nair R, et al. Design and microwave assisted synthesis of coumarin derivatives as PDE inhibitors. Int J Med Chem. 2016;9890630

Kuo PL, Chiang HS, Wang YY, Kuo YC, Chen MF, Yu IS, et al. SEPT12-Mictotubules complexesare required for sperm head and tail formation. Int J Mol Sci. 2013;14:22102–1.

DOI: https://doi.org/10.15395/mkb.v50n2.1238

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