Serum Albumin Levels of Oral Candidiasis Immunosuppressed Rats Treated With Hyperbaric Oxygen

Agni Febrina Pargaputri; Dwi Andriani

doi:10.15850/ijihs.v8n1.2086

Serum Albumin Levels of Oral Candidiasis Immunosuppressed Rats Treated With Hyperbaric Oxygen

Agni Febrina Pargaputri, Dwi Andriani

Abstract

Objective: To investigate serum albumin levels in oral candidiasis immunosuppressed rats treated with hyperbaric oxygen. One of the predisposing factors for oral candidiasis was the use of immunosuppressive drugs continuously. It can also affect the work of the liver because it’s one of the organs responsible for drug metabolism. Hyperbaric oxygen therapy was used not only to suppressing fungal infections, but also to improve liver function by evaluating the serum albumin levels.

Methods: This study used a post-test only control group design. Fifteen Wistar rats were divided into 3 groups(n=5/3): G1 (healthy group), G2 (oral candidiasis immunosuppressed rats group without hyperbaric oxygen therapy), and G3 (oral candidiasis immunosuppressed rats group with hyperbaric oxygen therapy). G2 and G3 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetracycline 1 mg/day/rat. Hyperbaric oxygen therapy was given to the G3 group in 5 days. Blood serum of rats in all groups was taken to calculate albumin levels.

Results: The average value of albumin levels in G2 group showed a decrease compared to the G1 group, while G3 showed the highest level. One way Anova test showed a significant difference among groups (p<0,05). To compare the difference between each group we used LSD test and showed a significant difference (p<0,05) between G1 compared to G2, G1 compared to G3, and G2 compared to G3.

Conclusion: Liver albumin levels of oral candidiasis immunosuppressed rats treated with hyperbaric oxygen therapy showed higher levels than those without therapy.

Keywords

Albumin; hyperbaric oxygen; immunosuppressed; liver; oral candidiasis

Full Text:

PDF

References

Vila T, Sultan AS, Montelongo-Jauregui D, Jabra-Rizk MA. Oral candidiasis: a disease of opportunity. J Fungi (Basel). 2020;6(1):15.

Zhang M, Yang X, Wang D, Yu C, Sun S. Antifungal activity of immunosuppressants used alone or in combination with fluconazole. J Appl Microbiol. 2019;126(5):1304–17.

Keerthi M, Reddy GS, Shekar PC, Chandra KL, Kumar KK, Reddy B. A study on isolation, identification, and antifungal susceptibility of various oral candidal species in renal transplant patients. J NTR Univ Health Sci [serial online]. 2015 [cited 2020 Jul 1];4:170-5. Available from: http://www.jdrntruhs.org/text.asp?2015/4/3/170/165399.

Jeanne Louise Schonborn. The role of the liver in drug metabolism. Anaesthesia Tutorial of the Week. [cited 2020 Jul 2] Available from: https://www.wfsahq.org/components/com_virtual_library/media/f7d652ac786de56a4a5832208294e382-ac7f3d7a04a43cfa854d58102cd229eb-179-The-role-of-the-liver-in-drug-metabolism.pdf

Prasetiawan E, Sabri E, Ilyas S. Gambaran histologis hepar mencit (Mus musculus L.) strain ddw setelah pemberian ekstrak n-heksan buah andaliman (Zanthoxylum acanthopodium Dc.) Selama masa pra implantasi dan pasca implantasi. Jurnal Online Saintia Biologi. 2012; 1(1):1-6.

Philip Hall and Johnny Cash. What is the real function of the liver ‘function’ tests?. Ulster Medical Journal. 2012;81(1):30–6.

George Kasarala, Hans L. Tillmann. Standard liver tests. Clinicla Liver Disease a Multimedia Review Journal. 2016;8(1):13–8.

Joana R. Carvalho, Mariana Verdelho-Machado. New insights about albumin and liver disease. Ann Hepatol. 2018;17(4):547–60.

Anna Skiada, Fanny lanternier, Andreas H. Groll, Livio Pagano, Stephen Zimmerli, Raoul Herbrecht, et al. Diagnosis and treatment of mucormycosis in patients with hematological malignancies: guidlines from the 3rd European conference on infections in leukimia (ECIL 3). Haematologica. 2013:98(4):492–501.

Fanny Margaretha Laihad, I Ketut Sudiana, M Guritno. Hyperbaric Oxygen Therapy on mucormycosis infection in oral cavity. Folia Medica Indonesiana. 2017; 53(2):163–8.

Agni Febrina Pargaputri dan Dwi Andriani. Pengaruh pemberian terapi oksigen hiperbarik terhadap jumlah limfosit darah pada kandidiasis oral imunosupresi model. Denta Jurnal Kedokteran Gigi. 2018;12(2):36.

Yun Sun, Yankai Wen, Chanjuan Shen, Yuanrun Zhu, Wendong You, Yuanyuan Meng, et al. Hyperbaric oxygen therapy in liver disease. Int J Med Sci. 2018;15(8):782–7.

Andriani D, Pargaputri AF. Enhance of IL-22 expression in oral candidasis immunosuppressed model with Acanthus ilicifolius extract therapy. IOP Conference Series : Earth and Environmental Science 217. 2019:1–6.

Irawan H, Semadi IN, Devi A. Effect of hyperbaric oxygen therapy to improve serum albumin for patients with diabetic foot ulcers. Biomed Pharmacol J. 2018;11(1):569–75.

Bernardi M, Maggioli C, Zaccherini G. Human albumin in the management of complication in liver chirrosis. Critical Care. 2012;16(211):1–7.

Rosida A. Pemeriksaan laboratorium penyakit hati. Berkala Kedokteran. 2016;12(1):123–31.

Saad RA, FathelBab MF, El-Saba AA, Shalaby. AA. The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats. Journal of Taibah University for Science. 2016;10(6):877–86.

MY Taslipinar, I Aydin, U Kaldirim, FN Aydin, M Agilli, YE Eyi, et al. Hyperbaric oxygen treatment and N-acetylcysteine ameliorate acetaminophen-induced liver injury in a rat model. Hum Exp Toxicol. 2013;32(10):1107–16.

Garcovich M, Zocco MA, Gasbarrini A. Clinical use of albumin in hepatology. Blood Transfus. 2009;7(4):268–77.

Prameswari N, Sunaryo IR, Damayanti DW, Pargaputri AF. The influence of hyperbaric oxygen therapy (HBOT) to intercausal relationship between blood vessels, osteoblast, and new bone formation during maxillary suture expansion. Padjadjaran Journal of Dentistry. 2020; 32 (1):14–21.

Dirmadana RA, Mediani GS, Sandana IKI, Alief F, Yasin JJ, Brahmanta A. Inovasi Stichopus hermanii dan TOHB dalam meningkatkan jumlah fibroblas pada ligamen periodontal. Denta Jurnal Kedokteran Gigi. 2017;11(1):15–24.

Yasin JJ, Brahmanta A, Pargaputri AF. Inovasi terapi oksigen hiperbarik dan Stichopus hermanii terhadap jumlah makrofag pada ligamen periodontal antara daerah tekanan dan tarikan selama pergerakan gigi ortodonti. Denta Jurnal Kedokteran Gigi. 2018;12(1):1–10.

Arroyo V, Clària J. Acute-on-Chronic liver failure, human serum albumin, and immune modulation: the beginning of an exciting adventure. Clin Gastroenterol Hepatol. 2018;16(5):633-636.

DOI: https://doi.org/10.15850/ijihs.v8n1.2086