Prevalence of Trichomonas vaginalis Based on Clinical Manifestation and Polymerase Chain Reaction among Reproductive Women

Huriyah Hafizhotul 'Ummah; Gita Widya Pradini; Reiva Farah Dwiyana; Muhammad Ersyad Hamda

doi:10.15850/ijihs.v7n1.1490

Prevalence of Trichomonas vaginalis Based on Clinical Manifestation and Polymerase Chain Reaction among Reproductive Women

Huriyah Hafizhotul 'Ummah, Gita Widya Pradini, Reiva Farah Dwiyana, Muhammad Ersyad Hamda

Abstract

Objective: To measure the prevalence of Trichomonas vaginalis (T. vaginalis) based on clinical manifestations and polymerase chain reaction (PCR) among reproductive women.

Methods: Subjects of the study were the vaginal swab obtained from reproductive women who attended the gynecology examination at Kandanghaur and Sindang primary health care of Indramayu District, West Java in 2016. This study was a descriptive study with cross-sectional method. Sampling was performed with total sampling method and 76 of vaginal swabs were included in this study. The prevalence of T. vaginalis was measured using PCR. The vaginal specimens were collected and then processed for PCR analysis using TVK3/TVK7.

Results: Prevalence of T. vaginalis among reproductive women in Indramayu District, West Java that analyzed using PCR was 0%. This result could be affected by the study setting in community, presence or absence of symptoms, and population studied.

Conclusion: There were no positive results of T. vaginalis, suggested by the samples that obtained from community-based of a low-risked population.

Keywords: Trichomonas vaginalis, polymerase chain reaction, prevalence, reproductive women

Keywords

Trichomonas vaginalis; polymerase chain reaction; prevalence; reproductive women

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References

Ryu JS, Min DY. Trichomonas vaginalis and trichomoniasis in the Republic of Korea. Korean J Parasitol. 2006;44(2):101–16.

Menezes CB, Frasson AP, Tasca T. Trichomoniasis - are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide? Microb Cell. 2016;3(9):404–19.

Kissinger P. Trichomonas vaginalis: a review of epidemiologic, clinical and treatment issues. BMC Infect Dis. 2015;15(1):1–8.

Harp DF, Chowdhury I. Trichomoniasis: evaluation to execution. Eur J Obstet Gynecol Reprod Biol. 2011;157(1):3–9.

Queza MI, Rivera WL. Diagnosis and molecular characterization of Trichomonas vaginalis in sex workers in the Philippines. Pathog Glob Health. 2013;107(3):136–40.

Bouchemal K, Bories C, Loiseau PM. Strategies for Prevention and treatment of Trichomonas vaginalis Infections. Clin Microbiol Rev. 2017;30(3):811–25.

Johnston VJ, Mabey DC. Global epidemiology and control of Trichomonas vaginalis. Curr Opin Infect Dis. 2008;21(1):56–64.

Patil MJ, Nagamoti JM, Metgud SC. diagnosis of Trichomonas vaginalis from vaginal specimens by wet mount microscopy, in pouch

TV culture system, and PCR. J Glob Infect Dis. 2012;4(1):22–5.

Muzny CA, Schwebke JR. The clinical spectrum of Trichomonas vaginalis infection and challenges to management. Sex Transm Infect. 2013;89(6):423–5.

Hobbs MM, Seña AC. Modern diagnosis of Trichomonas vaginalis infection. Sex Transm Infect. 2013;89(6):434–8.

Conrad MD, Gorman AW, Schillinger JA, Fiori PL, Arroyo R, Malla N, et al. Extensive genetic diversity, unique population structure and evidence of genetic exchange in the sexually transmitted parasite Trichomonas vaginalis. Plos Neglect Trop Dis [serial on the internet]. 2012 Mar [cited 2017 Jan 20];6(3):[about 11p.]. Available from: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001573.

Fernando SD, Herath S, Rodrigo C, Rajapakse L. Clinical features and sociodemographic factors affecting Trichomonas vaginalis infection in women attending a central sexually transmitted diseases clinic in Sri Lanka. Indian J Sex Transm Dis AIDS. 2012;33(1):25–31.

Gavgani AM, Namazi A, Ghazanchaei A, Alizadeh S. Prevalence and risk factors of trichomoniasis among women in Tabriz. Iranian J Clin Infect Dis. 2008;3(2):67–71.

Fernando SD, Herath S, Rodrigo C, Rajapakse S. Improving diagnosis of Trichomonas vaginalis infection in resource limited health care settings in Sri Lanka. J Glob Infect Dis. 2011;3(4):324–8.

DOI: https://doi.org/10.15850/ijihs.v7n1.1490