Infused Oyster Mushroom (Pleurotus ostreatus) Inhibits Glucose Absorption through Intestinal Mucosal Membrane in Wistar Rats
Abstract
Objective: To observe the effects of infused oyster mushroom (latin name: Pleurotus ostreatus) on glucose absorption through the intestinal mucosal membrane in Wistar rats.
Methods: This study used experimental design. Subjects of this study were six Wistar rats which were randomly divided into two groups; three rats were used for the analysis of the most effective time for glucose absorption and the other three were used to evaluate the effect of infused oyster mushroom on glucose absorption. The intestine of subjects was connected to in situ perfusion machine. Control glucose solution (Ctr) or glucose solution containing 5% (Oys 5%), 10% (Oys 10%), and 20% (Oys 20%) infused oyster mushroom was added into the perfusion machine and flowed back and forth in the lumen of intestine subsequently, accompanied by washing procedures using 0.9% NaCl between treatments. The glucose concentration was determined using deproteinization method followed by absorbance measurement. Student’s t test was performed to analyze the difference between groups.
Results: The level of glucose absorbed through intestinal mucosal membrane in control glucose solution and glucose solution with different concentrations of infused oyster mushroom, i.e. 5%, 10% or 20%, was 25.19+3.4 mg/dL, 16.27+0.86 mg/dL, 13.22+1.58 mg/dL and 10.03+1.25 mg/dL, respectively. Student’s t test showed significant differences between groups; Ctr and Oys 5% (p=0.03), Ctr and Oys 10% (p=0.02), and Ctr and Oys 20% (p=0.007).
Conclusion: Infused oyster mushroom inhibits glucose absorption through intestinal mucosal membrane in Wistar rats.
Keywords: Oyster mushroom (Pleurotus ostreatus), glucose absorption, intestine
DOI: 10.15850/ijihs.v6n2.1354
Keywords
Full Text:
PDFReferences
Gorboulev V, Schürmann A, Vallon V, Kipp H, Jaschke A, Klessen D, et al. Na+-d-glucose cotransporter sglt1 is pivotal for intestinal glucose absorption and glucose-dependent incretin secretion. Diabetes. 2012;61(1):187–96.
Thorens B. GLUT2, glucose sensing and glucose homeostasis. Diabetologia. 2015;58(2):221–32.
Roder PV, Geillinger KE, Zietek TS, Thorens B, Koepsell H, Daniel H. The role of SGLT1 and GLUT2 in intestinal glucose transport and sensing. PLoS One [serial on the internet]. 2014 Feb [cited 2018 Mar 20];9(2):[about 10p.]. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089977.
Bakke AM, Chikwati EM, Venold FF, Sahlmann C, Holm H, Penn MH, et al. Bile enhances glucose uptake, reduces permeability, and modulates effects of lectins, trypsin inhibitors and saponins on intestinal tissue. Comp Biochem Physiol A Mol Integr Physiol. 2014;168(1):96–109.
Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol. 2018;14(2):88–98.
Wu T, Xie C, Wu H, Jones KL, Horowitz M, Rayner CK. Metformin reduces the rate of small intestinal glucose absorption in type 2 diabetes. Diabetes Obes Metab. 2017;19(2):290–3.
Paravamsivam P, Heng CK, Malek SN, Sabaratnam V, M RR, Kuppusamy UR. Giant oyster mushroom pleurotus giganteus (agaricomycetes) enhances adipocyte differentiation and glucose uptake via activation of pparγ and glucose transporters 1 and 4 in 3t3-l1 cells. Int J Med Mushrooms. 2016;18(9):821–31.
Patel Y, Naraian R, Singh VK. Medicinal properties of pleurotus species (oyster mushroom): a review. World J Fungal Plant Biol. 2012;3(1):1–12.
Zhang Y, Hu T, Zhou H, Zhang Y, Jin G, Yang Y. Antidiabetic effect of polysaccharides from Pleurotus ostreatus in streptozotocin-induced diabetic rats. Int J Biol Macromol. 2016;83(1):126–32.
Thomas PA, Geraldine P, Jayakumar T. Pleurotus ostreatus, an edible mushroom, enhances glucose 6-phosphate dehydrogenase, ascorbate peroxidase and reduces xanthine dehydrogenase in major organs of aged rats. Pharm Biol. 2014;52(5):646–54.
Gu YH, Sivam G. Cytotoxic effect of oyster mushroom Pleurotus ostreatus on human androgen-independent prostate cancer PC-3 cells. J Med Food. 2006;9(2):196–204.
Windholz M. The merck index: an encyclopedia of chemicals, drugs and biologicals, 10th ed. New Jersey: Merck and Co; 1983.
Wu T, Horowitz M, Rayner CK. New insights into the anti-diabetic actions of metformin: from the liver to the gut. Expert Rev Gastroenterol Hepatol. 2017;11(2):157–66.
Rena G, Pearson ER, Sakamoto K. Molecular mechanism of action of metformin: old or new insights? Diabetologia. 2013;56(9):1898–906.
DOI: https://doi.org/10.15850/1354
Article Metrics
Abstract view : 1026 timesPDF - 264 times
This Journal indexed by
IJIHS is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
View My Stats