Solanum muricatum Aiton Juice as A Hepatoprotective Agent in Wistar Rats Induced With Carbon Tetrachloride
Abstract
Background: Liver participates in various metabolic processes in human body. Exposures to toxins such as carbon tetrachloride (CCl4) results in hepatocyte destruction and release the cell contents. Enzymes such as serum glutamic pyruvic transaminase (SGPT) were used as a parameter to diagnose liver damage. Pepino (Solanum muricatum Aiton) contains antioxidants that protect liver from hepatoxicity. The aim of this experiment is to determine the effect of pepino in protecting the hepatocyte from hepatotoxic effect of CCl4.
Methods: A total of 16 Wistar rats used as the subject were divided into Control and Therapeutic Group. The Control group was induced with CCl4 but was not given pepino juice. Meanwhile, the Therapeutic Group was given pepino juice for 10 days. For induction of hepatotoxicity, CCl4 10% was given at a dosage of 2.0 ml/kg intra-peritoneal. Each Wistar rat in Therapeutic Group was given 1.0 ml of 300 g/ml pepino juice via oral feeding. Spectrophotometer with adjusted wavelength of 340 nm was used to measure SGPT level of the Wistar rats and statistical T-test was used to analyze the data.
Results: Wistar rats in Therapeutic group showed a larger decrease (6898.9 IU/L–79.1 IU/L) in SGPT level compared to the Control group (6469.8 IU/L–418.5 IU/L). The SGPT level of the Wistar rat in Therapeutic group reached normal baseline (50 IU/L–150 IU/L). The significance of the experiment was supported by the T-test, P-value <0.05
Discussion: Pepino juice had antioxidants that protect the hepatocyte of Wistar rats from free radicals. The synergistic action of antioxidants and hepatocyte regeneration of Wistar rats in Therapeutic group caused a decrease in SGPT level. Therefore, the experiment concluded that pepino juice at the dosage of 300 g/ml has hepatoprotective effect.
Key words: Carbon tetrachloride, hepatotoxicity, Pepino, Solanum muricatum Aiton, SGPT
DOI: 10.15850/amj.v2n4.484
Keywords
Full Text:
PDFReferences
Akram E, Olamafar S, Zaringhalam J, Rezazadeh S, Eidi M. Protective effect of Walnut (Juglans regia L.) extract against CCl4 – induced hepatotoxicity in rats. Pejouhesh. 2011;35(2):87–92.
Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol. 2003;33(2):105–36.
Navarro VJ, Senior JR. Drug-Related Hepatotoxicity. N Engl J Med. 2006;354(7):731–9.
Dooley JS, Lok A, Burroughs A, Heathcote J, editors. Sherlock's Diseases of the Liver and Biliary System. 12th ed. Oxford:Wiley-Blackwell; 2011.
McPherson RA, Pincus MR, Henry JB. Henry's Clinical Diagnosis and Management by Laboratory Methods. 21st ed. Philadelphia: Saunders Elsevier; 2007.
Giboney PT. Mildly elevated liver transaminase levels in the asymptomatic patient. Am Fam Physician. 2005;71(6):1105–10.
Hsu C, Guo Y, Wang Z, Yin M. Protective effects of an aqueous extract from pepino (Solanum muricatum Ait.) in diabetic mice. J Sci Food Agric. 2011;91(8):1517–22.
Arindah D. Fractionation and Identification of Compound In Fruit Meat of Solanum muricatum Aiton that has Potential as Antioxidants [dissertation]. Malang: Universitas Islam Negeri Maulana Malik Ibrahim; In Press 2010.
Sudha G, Priya MS, Shree RB, Vadivukkarasi S. Antioxidant activity of ripe and unripe pepino fruit (Solanum muricatum Aiton). J Food Sci. 2012;77(11):1131–5.
Saptarini N.M., Suryasaputra D., A.M. S. Analisis Rasio Proteksi Antiulser Sari Buah Pepino (Solanum muricatum Aiton) Menggunakan Mencit sebagai Model Hewan Coba. Majalah Obat Tradisional. 2011;16 (2):75–80.
Galal RM, Zaki HF, Seif El-Nasr MM, Agha AM. Potential protective effect of honey against paracetamol-induced hepatotoxicity. Arch Iran Med. 2012;15(11):674–80.
Feng Y, Siu K, Ye X, Wang N, Yuen M, Leung C, et al. Hepatoprotective effects of berberine on carbon tetrachloride-induced acute hepatotoxicity in rats. Chinese medicine. 2010;5(1):1–6.
Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in small laboratory animals. J Pharmacol Pharmacother. 2010;1(2):87–93.
Domitrović R, Jakovac H, Blagojević G. Hepatoprotective activity of berberine is mediated by inhibition of TNF-α, COX-2, and iNOS expression in CCl4 intoxicated mice. Toxicology. 2011;280(1-2):33–43.
Wong LL, Fan ST, Man K, Sit WH, Jiang PP, Jor IW, et al. Identification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicity. Hum Exp Toxicol. 2011;30(9):1369–81.
Article Metrics
Abstract view : 463 timesPDF - 232 times
This Journal indexed by
AMJ is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
View My Stats