Propolis of Trigona spp. Protects Mucosa from Aspirin-Induced Gastric Mucosal Damage in Rats

Achadiyani Achadiyani, Anindita Laksmi, Dolvy Girawan


Background: Helicobacter pylori and non-steroid anti-inflammatory drugs are the major causes of peptic ulcer in the world. Indonesian native stingless bee species, Trigona spp., produces propolis that might be effective to protect mucosal damage. The aim of the study was to determine the protective effect of Trigona spp. propolis on aspirin-induced gastric mucosal damage in rats.

Methods: This experimental study was conducted from September–November 2013 at Animal Laboratory of Department of Pharmacology and Therapy Faculty of Medicine Universitas Padjadjaran. Healthy male Wistar rats (n=24) aged 2–3 months old and weighed 200–250 grams were randomly divided into three groups. The first group was control negative, the second group was given 100 mg/kg body weight of aspirin, and the third group was given 200 mg/kg body weight of Trigona spp. propolis, one hour before administration of 100 mg/kg body weight of aspirin. After two weeks of treatment, rats were sacrificed by laparotomy to obtain gastric tissues, followed by processing for the paraffin section for histopathological analysis. The grade of gastric mucosal damage was determined under a light microscope. Data were then compared between groups using the Mann-Whitney test.

Results: Oral administration of aspirin-induced gastric mucosal damage ranging from grade 0 to grade IV; whereas administration of propolis showed a reduction of gastric mucosal damage’s grade when compared to the aspirin group (p<0.05).

Conclusions: Trigona spp. propolis has a protective effect on aspirin-induced gastric mucosal damage. Further study is encouraged to study an optimal dose of aspirin after propolis administration.



Aspirin; gastric mucosal damage, propolis; Trigona spp

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Amin D. A textbook of clinical pharmacology and therapeutics. Ritter JM, Lewis LD, Mant TGK, Ferro A, editors. A textbook of clinical pharmacology and therapeutics. 5th Ed. Great Britain: Hodder Arnold; 2008. p. 247.

Soll AH, Graham DY. Peptic ulcer disease. Yamada T, Alpers DH, Kalloo AN, Kaplowitz N, Owyang C, Powell DW, editors. Textbook of Gastroenterology. 5th Ed. West Sussex: Wiley Blackwell; 2009. p. 936–73.

Yeomans ND. Aspirin: old drug, new uses and challenges. J Gastroenterol Hepatol. 2010;26(3):426–31.

Musumba C, Pritchard DM, Pirmohamed M. Review article: cellular and molecular mechanisms of NSAID-induced peptic ulcers. Aliment Pharmacol Ther. 2009;30:517–31.

Mahani, Karim RA, Nurjanah N, Dahlianti R. Keajaiban Propolis Trigona. 1st Ed. Jakarta: Pustaka Bunda; 2011. p. 33–55

Lotfy M. Biological activity of bee propolis in health and disease. Asian Pacific J Cancer Prev. 2006;7:22–31.

Seleem HS. Effect of aspirin versus aspirin and vitamin C on gastric mucosa (fundus) of adult male albino rats. histological and morphometric study. Egypt J Histol. 2010;33(2):313–26.

Pillai SI, Kandaswamy M, Subramanian S. Antiulcerogenic and ulcer healing effects of Indian propolis in experimental rat ulcer models. J Api Prod Api Med Sci. 2010;2(1):21–8.

Morini G, Grandi D. Methods to measure gastric mucosal lesion in the Rat. Curr Protoc Toxicol. 2010;43:1–15.

Wallace JL. Prostaglandin, NSAID, and gastric mucosal protection : why doesn’t the stomach digest itself. Physiol Rev. 2008;88(4):1547–65.

Narayana KR, Reddy MS, Chaluvadi MR. Bioflavonoid classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol. 2001;33(1):2–16.

Mota KS, Dias GE, Pinto ME, Luiz-Ferreira Â, Souza-Brito AR, Hiruma-Lima CA, et al. Flavonoids with gastroprotective activity. Molecules. 2009;14(3):979–1012.

Siregar HC, Fuah AM, Octavianty Y. Propolis: madu multikhasiat. 1st Ed. Jakarta: Penerbit Swadaya; 2011.p. 35

De Barrosa MP, Sousab JP, Bastosb JK, de Andradea SF. Effect of Brazilian green propolis on experimental gastric ulcers in rats. J Ethnopharmacol. 2007;110(3):567–71.

Mohafez OMM, Abdel-Raheem IT, Nafady AM. Antioxidant, lipid peroxidation-inhibitory and antiulcer activities of brown propolis. Bull Pharm Sci. 2010;33:169–77.

Lemos M, de Barros MP, Sousa JP, da Silva Filho AA, Bastos JK, de Andrade SF. Baccharis dracunculifolia, the main botanical source of brazilian green propolis, displays antiulcer activity. J Pharm Pharmacol. 2007;59(4):603–8.


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