Antibacterial Effect of Jatropha multifida L. Leaf Infusion towards Staphylococcus aureus and Pseudomonas aeruginosa
Abstract
Background: Jatropha multifida is one of the medicinal plants commonly found in Indonesia. This plant is used in the community to heal open wounds, however, scientific evidence is lacking. The two most common bacteria which often cause infection in open wounds are Staphylococcus aureus and Pseudomonas aeruginosa. This study aimed to determine the antibacterial effect of J. multifida leaf infusion towards S. aureus and P. Aeruginosa in vitro.
Methods: This was an experimental laboratory study conducted at the Microbiology Laboratory, Faculty of Medicine, Universitas Padjadjaran in 2014. The modified Kirby-Bauer antimicrobial diffusion procedure on Mueller-Hinton agar was applied to determine the inhibitory zone. In determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), the modified technique of tube dilution was used.
Results: The results of this research showed that the infusion of J. multifida leaves had inhibitory effects on the growth of S. aureus dan P. aeruginosa at the concentration of 100% and 75%. The minimum inhibition concentration and minimum bactericidal concentration could not be determined.
Conclusions: There is evidence confirming the bacteriostatic antibacterial effect of J. multifida leaves which inhibits the growth of S. aureus and P. Aeruginosa. Further study is needed to explore J. multifida leaves.
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Mina EC, Ibarra MR, Franzblau SG, Aguinaldo AM. Chemical and anti-tubercular screening on the leaves of Jatropha multifida Linn. Pure Appl Bio. 2014;2(1):32–6.
Falodun A, Igbe I, Erharuyi OY, Agbanyim OJ. Chemical characterization, anti inflammatory and analgesic properties of Jatropha multifida root bark. J Appl Sci Environ Manag. 2013;17(3):357–62.
Agoes Goeswin. Teknologi bahan alam. Bandung: Penerbit ITB; 2007. p.10–24.
Muntiaha MC, Yamlean PVY, Lolo WA. Uji efektivitas sediaan krim getah jarak cina (Jatropha multifida L.) untuk pengobatan luka sayat yang terinfeksi bakteri Staphylococcus aureus pada kelinci (Orytolagus cuniculus). PHARMACON. 2014;3(3):294–302.
Dwiprahasto I. Kebijakan untuk meminimalkan risiko terjadinya resistensi bakteri di unit perawatan intensif rumah sakit. JMPK. 2005;8(4):177–81.
Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology. 4th ed. USA: Saunders Elsevier; 2011. p.280–1.
Falodun A, Imieje V, Erharuyi O, Joy A, Langer P, Jacob M, et al. Isolation of antileishmanial, antimalarial and antimicrobial metabolites from Jatropha multifida. Asian Pac J Trop Biomed. 2014;4(5):374–8.
Aransiola MN, Ehikhase C, Mmegwa JC, Wahab IO. Antibacterial and antifungal activities of Jatropha multifida (ogege) sap against some pathogens. IOSR J Pharm Biol Sci. 2014;9(4):53–7.
Nuria MC, Faizatun A, Sumantri. Uji aktivitas antibakteri ekstrak etanol daun jarak pagar (Jatropha curcas L) terhadap bakteri Staphylococcus aureus ATCC 25923, Escheria coli ATCC 25922, dan Salmonella typhi ATCC 1408. MEDIAGRO. 2009;5(2):26–37.
Fahriya PS, Shofi MS. Ekstraksi zat aktif antimikroba dari tanaman yodium (Jatropha multifida L) sebagai bahan baku alternatif antibiotik alami. JTKI. 2011:1–7.
Agustiningsih, Wildan A, Mindaningsih. Optimasi cairan penyari pada pembuatan ekstrak daun pandan wangi (Pandanus amaryllifous R) secara maserasi terhadap kadar fenolik dan flavonoid total. MOMENTUM. 2010;6(2):36–41.
DOI: https://doi.org/10.15850/amj.v6n2.1622
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