Effect of Sappan Wood Ethanol Extracts (Caesalpinia sappan. L) to the Sperm Motility, Viability, and Concentration of Male Wistar Rats

Nadiyah Nadiyah, Andri Rezano, Sunarjati Sudigdoadi

Abstract


Background: Idiopathic male infertility becomes an issue because of the limited management options and increasing number of infertile couples. Sappan wood (Caesalpinia sappan. L) is an herbal plant predicted to have fertility and spermatogenesis enhancing effects through antioxidant properties. This study aimed to observe the effects of sappan wood ethanol extract in improving sperm quality of male wistar rat based on sperm motility, viability, and concentration.

Methods: This was an experimental laboratory study by using sappan wood ethanol extracts as material and male wistar rat as the experimental animal. Twenty-four adult male wistar rats were divided into 4 groups including rats which were treated with aquades, 30, 60, and 120 mg/kg/day/orally of ethanol extract of sappan wood, respectively, during 35 days. After this period, the animals were sacrificed. Then the epididymis were taken for samples to calculate sperm motility percentage, sperm viability percentage and sperm concentration. Comparison between control group and the treated group was analyzed by one-way Annova.

Results: There were significant differences between the control group and the group with 120 mg/kg of sappan wood ethanol extracts in the percentage of motility (36.39% and 51.75%; p-value 0.001), viability percentage (55.36% and 66.63%, p-value 0.031), and sperm concentration (54.47 million/ml and 47.79 million/ml; p-value 0.001).

Conclusions: The ethanol extract of sappan wood with a dose of 120 mg/kg for 35 days is able to improve sperm motility percentage, sperm viability percentage, and sperm concentration of male wistar rat.


DOI: 10.15850/amj.v4n2.1078


Keywords


Antioxidant, Caesalpinia sappan. L, infertility

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References


Diemer T, Kopa Z, Dohle G, et al. European Association of urology guidelines on male infertility: the 2012 update. Eur Urol. 2012;62(2):324–32.

Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLos Med. 2012;9(12):e1001356.

Hasanpoor-Azghdy SB, Simbar M, Vedadhir A. The emotional-psychological consequences of infertility among infertile women seeking treatment : a result of qualitative study. Iran J Reprod Med. 2014;12(2):131–8.

Kim SJ, Kim MR, Hwang SY, Bae WJ, Kim S, Hong SH, et al. Preliminary report on the safety of a new herbal formula and its effect on sperm quality. World J Mens Health. 2013;31(3):254–61.

Imamovic Kumalic S, Pinter B. Review of Clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. Biomed Res Int. 2014;21(2):190–5.

Nirmal NP, Rajput MS, Prasad RG, Ahmad M. Brazilin from Caesalpinia sappan heartwood and its pharmacological activities: a review. Asian Pac J Tropmed. 2015;8(6):421–30.

Fujii J, Tsunoda S. Redox regulation of fertilisation and the spermatogenic process. Asian J Androl. 2011;13(3):420–3.

Kementrian Kesehatan Republik Indonesia. Suplemen I farmakope herbal Indonesia. Jakarta: Kementrian Kesehatan Republik Indonesia; 2010.

WHO. WHO laboratory manual for the examination and processing of human semen [Internet]. 2010 [cited 12 Mei 2015]. Available from : http://www.who.int/reproductivehealth/publications/infertility/9789241547789/

Min BS, Cuong TD, Hung TM, Min BK, Shin BS, Woo MH. Compounds from the heartwood of Caesalpinia sappan and their anti-inflammatory activity. Bioorg Med Chem Lett. 2012;22(24):7436–9 .

Bae I-K, Min H-Y, Han A-R, Seo E-K, Lee SK. Suppression of lipopoly saccharide-induced expression of inducible nitric oxide synthase by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol. 2005;513(3):237– 42.

Sasaki Y, Hosokawa T, Nagai M, Nagumo S. In vitro study for inhibition of NO production about constituents of Sappan Lignum. Biol Pharm Bull. 2007;30(1):193–6.

Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem. 2006;99(1):191–203 .

Tremellen K. Oxidative stress and male infertility––a clinical perspective. Hum Reprod Update. 2008;14(3):243–58.

Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol. 2009;16(5):449–57




DOI: https://doi.org/10.15850/amj.v4n2.1078

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