Efek Interval Training terhadap Indeks Lee, Kadar Adiponektin, dan IL-6 pada Tikus Model Obesitas

Endang Mulyana Zein, Vita Murniati T. Lubis, Ambrosius Purba


Kenaikan berat badan pada tikus model obesitas yang tetap diberikan pakan tinggi lemak terus berlangsung, akan tetapi jenis aktivitas fisik yang tepat mungkin dapat memperlambatnya. Penelitian ini bertujuan mengetahui perbedaan efek interval training tipe cepat, interval training tipe lambat, dan continuous training intensitas sedang terhadap berat badan, nilai indeks Lee, serta kadar adiponektin dan IL-6. Penelitian ini dilakukan di Laboratorium Farmakologi dan Terapi Fakutas Kedokteran Unpad pada bulan April–Mei 2014. Penelitian ini menggunakan posttest-only control group design pada 28 ekor tikus model obesitas (indek Lee >0,30), kadar adiponektin dan IL-6 plasma diperiksa dengan metode ELISA. Hasil penelitian menunjukkan perlakuan interval training tipe lambat lebih baik dibanding dengan interval training tipe cepat, continuous training intensitas sedang dan kontrol, yaitu kenaikan berat badan (2,34% vs 10,79% vs 4,49% vs 7,58%, p=0,000), penurunan kadar adiponektin (9,04 vs 10,27 vs 10,57 vs 7,24 ng/mL, p=0,000), glukosa (101,91±7,1 vs 113,63±6,4 vs 144,03±9,0 vs 82,24±5,9 mg/dL, p=0,000), trigliserida (28,07±3,3 vs 34,14±5,7 vs 42,00±4,9 vs 17,34±2,7 mg/dL, p=0,000), peningkatan kadar IL-6 (60,29±3,1 vs 54,55±2,1 vs 50,76±4,1 vs 56,36±2,9 pg/mL, p=0,000), dan penurunan nilai indeks Lee (7,3% vs 3,6% vs 6,4% vs 5,2%, p=0,000). Penelitian ini menyimpulkan bahwa aktivitas fisik interval training tipe lambat lebih baik dalam memperlambat kenaikan berat badan dan menurunkan nilai indeks Lee akibat peningkatan penggunaan adiponektin dan IL-6. [MKB. 2016;49(1):15–21]

Kata kunci: Adiponektin, IL-6, Indeks Lee, interval training, obesitas


Effects of Interval Training on Lee Index, Adiponectine, and Il-6 in Obese Rat Model

Weight gain increase in obese rats that are continuously fed with fat rich chow may be slowed downs by putting them on a training regimen. This study aimed to determine the differences between the effects of fast-type interval training, slow-type interval training, and moderate-intensity continuous training on weight gain, Lee index, adiponectine, and IL-6. Using a post-test only control group, this study involved 28 obese rats (Lee index values >0.30). Adiponectin and IL-6 levels were tested using ELISA. This research was conducted in Pharmacology and Therapeutic Laboratory of Faculty of Medicine Unpad in April–May 2014. The results showed that the slow-type interval training was the most effective training compared to the fast-type interval training, continuous training of moderate intensity, and control in slowing down the weight gain (2.34% vs 10.79% vs 4.49% vs 7.58%, p=0.000), which was also accompanied by the lowest level of blood glucose level (101.91±7.1 vs 113.63±6.4 vs 144.03±9.0 vs 82.24±5.9 mg/dL, p=0.000), triglyceride (28.07±3.3 vs 34.14±5.7 vs 42.00±4.9 vs 17.34±2.7 mg/dL, p=0.000) and adiponectin (9.04±0.5 vs 10.27±0.7 vs 10.57±0.7 vs 7.24±0.8 ng/ml, p=0.000) as well as the highest level of IL-6 (60.29±3.1 vs 54.55±2.1 vs 50.76±4.1 vs 56.36±2.9 pg/mL, p=0.000) and lowest Lee index score (7.3% vs 3.6% vs 6.4% vs 5.2%, p=0.000). The study concludes that the slow-type interval training is the best training when compared to other types of training in slowing down weight gain and lowering Lee index and increasing the use of adiponectin and IL-6. [MKB. 2016;49(1):15–21]

Key words: Adiponectin, IL-6, interval training, Lee index, obese

DOI: 10.15395/mkb.v49n1.983



Adiponektin, IL-6, Indeks Lee, interval training, obesitas

Full Text:



WHO. 2008–2013 Action plan for the global strategy for the prevention and control of noncommunicable diseases. Physical Inactivity. 2008;61(8):19–29.

Boutcher SH. High-intensity intermittent exercise and fat loss. J Obes. 2011;2011(1):1–10.

Dunn SL. Effects of exercise and dietary intervention on metabolic syndrome markers of inactive premenopausal women [disertasi]. Sydney: University of New South Wales; 2009.

Campbell L, Wallman K, Green D. The effects of intermittent exercise on physiological outcomes in an obese population: continuous versus interval walking. J Sports Sci Med. 2010;9(1):24–30.

Bompa T, Haff GG. Periodization: theory and methodology of training. Edisi ke-5. Champaign: Human Kinetics; 2009.

Huang H, Iida KT, Sone H, Ajisaka R. The regulation of adiponectin receptors expression by acute exercise in mice. J Exp Clin Endocrinol Diabetes. 2007;115(7):417–22.

Alhusseini NF, Belacy NA, Kasem EM, Allam MM. Effect of exercise training on adiponectin receptor expression and insulin resistance in mice fed a high fat diet. J Am J Biochem Biotechnol. 2010;6(2):77–83.

Holland WL, Miller RA, Wang ZV, Sun K, Barth BM, Bui HH, dkk. Receptor-mediated activation of ceramidase activity initiates the pleiotropic actions of adiponectin. J Nat Med. 2011;17(1):55–63.

Tang H, Xie MH, Lei Y, Zhou L, Xu YP, Cai JG. The roles of aerobic exercise training and suppression IL-6 gene expression by RNA interference in the development of insulin resistance. J Cyto. 2013;61(2):394–405.

Scott JP, Sale C, Greeves JP, Casey A, Dutton J, Fraser WD. Effect of exercise intensity on the cytokine response to an acute bout of running. Med Sci Sports Exerc. 2011;43(12):2297–306.

Shahram S, Masoomeh K, Reza I, dkk. Adiponectin responses to continues and intermittent training in non-athlete obese women. J EJEBAU. 2011;1(4):216–20.

Helge JW, Klein DK, Andersen TM, van Hall G, Calbet J, Boushel R, dkk. Interleukin-6 release is higher across arm than leg muscles during whole-body exercise. Exp Physiol. 2011;96(6):590–8.

Baker JS, McCormick MC, Robergs RA. Interaction among Skeletal Muscle Metabolic Energy Systems during Intense Exercise. J Nutr Metab. 2010;2010:1–13.

Tang H, Xie MH, Lei Y, Zhou L, Xu YP, Cai JG. The roles of aerobic exercise training and suppression IL-6 gene expression by RNA interference in the development of insulin resistance. Cytokine. 2013;61(2):394–405.

Pedersen BK, Edward F. Adolph distinguished lecture: muscle as an endocrine organ: IL-6 and other myokines. J Appl Physiol. 2009;107(4):1006–14.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


This Journal indexed on:


Creative Commons License
MKB is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License


View My Stats