The Effect of 3-Days Storage and Temperature On ADP-induced Platelet Aggregation

Nanda Ayu Puspita, Suryawati Suryawati

Abstract


Objective:

Previous studies have revealed that platelet functions start to deteriorate after 3 days of storage, platelet activity may have changed earlier as a response to the exposure to various conditions during storage. The aim of this study is to observe platelet functions during 3 days of platelet storage and to evaluate the effect of storage temperature towards platelet aggregation response.

Material and Methods:

Two conditions of platelet storage were used in our study, at room temperature and cold condition at 4°C to observe the temperature effect on platelet aggregation. The aggregation test was performed using a 96-wells plate platelet aggregation method.

Results:

On Day 1, the response of platelet aggregation reduced to 34.9 ± 10.6%, which was less than half of the aggregation of fresh platelet (100%). The aggregation on day 2 reduced modestly (26.8 ± 4.2%), in comparison with that seen on the first day. On day 3, platelet aggregation deteriorated significantly (6 ± 1.2%), which was comparable to the control group without the presence of platelet agonist (6.1 ± 1.4%).

When platelets were stored at 4°C or at room temperature (22°C) platelet response to stimulation with ADP were different. The result showed that storing the blood at 4 ˚C or room temperature was not able to preserve platelet function, as the deterioration of platelet aggregation response over time was still taking place. Moreover, although the results was not statistically significant, our result indicated that cold storage might reduce platelet responsiveness toward ADP activation

Conclusion:

This current study provides evidence of the deterioration of platelet function during first 3 days of platelet storage. Moreover, we found that storing platelets in 4°C showed no significant benefit in preserving the ADP-induced platelet aggregation capacity compared to that stored in 22°C.


Keywords


Platelet; ADP; Platelet aggregation; Platelet storage

References


Ohto H, and Nollet KE. Overview on platelet preservation: Better controls over storage lesion. Transfusion and Apheresis Science 2011; 44(3):321-325.

Tynngård N. Preparation, storage and quality control of platelet concentrates. Transfusion and Apheresis Science 2009; 41(2):97-104.

Zhang JG, Carter CJ, Culibrk B, Devine DV, Levin E, Scammell K, et al. Buffy‐coat platelet variables and metabolism during storage in additive solutions or plasma. Transfusion 2008;48(5):847-856.

Nair PM, Pandya SG, Dallo SF, Reddoch KM, Montgomery RK, et al. Platelets stored at 4 degrees C contribute to superior clot properties compared to current standard-of-care through fibrin-crosslinking. Br J Haematol 2017;178(1):119-129.

Bynum JA, Adam M, Getz TM, Rodriguez AC, Aden JK, Cap AP, et al. Bioenergetic profiling of platelet mitochondria during storage: 4°C storage extends platelet mitochondrial function and viability. Transfusion 2016; 56:S76-S84.

Yang J, Yin W, Zhang Y, Sun Y, Ma T, Gu Set al. Evaluation of the advantages of platelet concentrates stored at 4° C versus 22° C. Transfusion 2018; 58(3):736-747.

Thon N, Schubert P, Devine DV. Platelet storage lesion: a new understanding from a proteomic perspective. Transfusion Medicine Reviews 2008;22(4):268-279.

Egidi MG, D’Alessandro A, Mandarello G, Zolla L. Troubleshooting in platelet storage temperature and new perspectives through proteomics. Blood Transfusion 2010; 8(Suppl 3):73-81.

Villarroel JPP, Figueredo R, Guan X, Tomaiuolo M, Karamercan MA, Welsh J, Selak MA, Becker LB, Sims C. Increased platelet storage time is associated with mitochondrial dysfunction and impaired platelet function. Journal of Surgical Research 2013; 184(1):422-429.

Garraud O, Cognasse F, Tissot JD, Chavarin P, Laperche S, Morel P, Lefrère JJ, Pozzetto B, Lozano M, Blumberg N. Improving platelet transfusion safety: biomedical and technical considerations. Blood Transfusion 2016:14(2):109.

Baimukanova G, Miyazawa B, Potter DR, Gibb SL, Keating S, Danesh A, Beyer A, Dayter Y, Bruhn R, Muench MO.The effects of 22 C and 4 C storage of platelets on vascular endothelial integrity and function. Transfusion 2016;56.

Podda GM, Scavone M, Femia EA, Cattaneo M, et al. Aggregometry in the settings of thrombocytopenia, thrombocytosis and antiplatelet therapy. Platelets 2018: 1-6.

Michelson AD. Methods for the measurement of platelet function. The American journal of cardiology 2009;103(3):20A-26A.

Vinholt PJ, Nybo M, Nielsen CB, Hvas AM, et al. Light transmission aggregometry using pre-coated microtiter plates and a Victor X5 plate reader. PloS one 2017;12(10):e0185675.

Dumont LJ, AuBuchon JP, Whitley P, Herschel LH, Johnson A, McNeil D, Sawyer S, Roger JC. Seven-day storage of single-donor platelets: recovery and survival in an autologous transfusion study. Transfusion 2002; 42(7):847-854.

Akay OM, Gündüz E, Başyiğit H, Gulbas Z. Platelet function testing during 5-day storage of single and random donor plateletpheresis. Transfusion and Apheresis Science 2007;36(3):285-289.

Reddoch KM, Pidcoke H, Montgomery RK, Fedyk CG, Aden JK, Ramasubramanian AK, Cap AP. Hemostatic function of apheresis platelets stored at 4 degrees C and 22 degrees C. Shock 2014; 41(Suppl 1): 54-61.

Shrivastava M. The platelet storage lesion. Transfusion and Apheresis Science 2009;41(2):105-113.

Braune S, Walter M, Schulz F, Lendlein A, Jung F. Changes in platelet morphology and function during 24 hours of storage. Clinical Hemorheology and Microcirculation 2014;58(1):159-70.

Diab A, Thomas A, Luban NLC, Wong ECC, Wagner SJ, Levy RJ. Acquired cytochrome C oxidase impairment in apheresis platelets during storage: a possible mechanism for depletion of metabolic adenosine triphosphate. Transfusion 2012;52(5):1024-1030.


Refbacks

  • There are currently no refbacks.




This journal indexed by:

            

  

Creative Commons License
IJIHS is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License



View My Stats