Effects of Calcium Bentonite on High Blood Cholesterol Level

Hendro Sudjono Yuwono; Fadhli Rajif Tangke; Reni Farenia

doi:10.15850/amj.v5n3.551

Effects of Calcium Bentonite on High Blood Cholesterol Level

Hendro Sudjono Yuwono, Fadhli Rajif Tangke, Reni Farenia

Abstract

Background: Calcium bentonite has been known to decrease high blood cholesterol level. This study aimed to explore whether a local calcium bentonite from West Java, Indonesia, had effect on lowering total blood cholesterol level.

Methods: A laboratory experimental study was carried out on thirty adult male Wistar rats’ at Laboratory animals, Department of Pharmacology and Therapy, Universitas Padjadjaran in 2012. The rats were divided into five groups with six rats in each group, consisting of group I (negative control) with standard diet and distilled water, group II (positive control) with high lipid diet and distilled water, group III with high lipid diet and ezetimibe 0.18 g/rat/day, groups IV with high lipid diet and calcium bentonite 0.25 g/rat/day, group V with high lipid diet and calcium bentonite 0.5 g/rat/day. Ezetimibe as was used as this was an inhibitory agent of intestinal cholesterol absorption. Blood cholesterol levels were measured and analyzed using analysis of variance and Tukey’s post-hoc test.

Results: Oral calcium bentonite at 0.25 g/rat/day and 0.5 g/rat/day significantly decrease total blood cholesterol level relative to the positive control group, 31.68% (p=0.018) and 32.87% (p=0.006) respectively, but its effect is inferior to ezetimibe 62.83% (p=0.000).

Conclusions: Local calcium bentonite from West Java, Indonesia, has a significant lowering effect, however, the effect is less comparing to ezetimibe.

Keywords

Blood cholesterol, calcium bentonite, ezetimibe, wistar rat

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References

Michelle Mcguire PD, Beerman KA. Nutritional Sciences: from fundamentals to Food. 3rd ed. Belmont: Cengage Learning; 2011.

Gershkovich P, Darlington J, Sivak O, Constantinides PP, Wasan KM. Inhibition of intestinal absorption of cholesterol by surface-modified nanostructured aluminosilicate compounds. J Pharm Sci. 2009;98(7):2390–400.

Sivak O, Darlington J, Gershkovich P, Constantinides PP, Wasan KM. Protonated nanostructured aluminosilicate (NSAS) reduces plasma cholesterol concentrations and atherosclerotic lesions in Apolipoprotein E deficient mice fed a high cholesterol and high fat diet. Lipids health dis. 2009;8(1):30.

Gershkovich P, Sivak O, Contreras-Whitney S, Darlington JW, Wasan KM. Assessment of cholesterol absorption inhibitors nanostructured aluminosilicate and cholestyramine using in vitro lipolysis model. J Pharm Sci. 2012;101(1):291–300.

Supeno M. Bentonit Terpilar dan Aplikasi. Medan: USU press; 2009.

Pusat Penelitian dan Pengembangan Teknologi Mineral dan Batubara. Informasi Mineral dan Batubara. Kelompok Program Teknologi Informasi Pertambangan; 2005 [cited 2012 May 4]; Available at: http://www.tekmira.esdm.go.id/data/Bentonit/ulasan.asp?xdir=Bentonit&commId=8&comm=Bentonit.

Katzung B, Masters S. Basic and Clinical Pharmacology. 12th ed. San Fransisco: McGraw-Hill; 2011.

European Chemical Bureau. IUCLID Dataset-Bentonite. Livingston: European Commission; 2000.

American Colloid Company. Volclay HPM20. Arlington Heights: Material Safety Data Sheet; 2008.

Hardiningsih R, Nurhidayat N. The effect of consuming the hypercholesterolemia rationed food to the body weight of white rats wistar with administration of lactic acid bacteria. Biodiversitas. 2006;7(2):127–30.

Eba F, Gueu S, Eya’A-Mvongbote A, Ondo JA, Yao BK, Ndong Nlo J, Biboutou RK. Evaluation of the absorption capacity of the natural clay from Bikougou (Gabon) to remove Mn (II) from aqueous solution. International Journal of Engineering Science and Technology. 2010;2(10):5001-16.

Moosavi M. Bentonite Clay as a Natural Remedy: a brief review. Iran J Public Health. 2017; 46(9):1176-83.

DOI: https://doi.org/10.15850/amj.v5n3.551