Lethal Dose of Calcium Bentonite in Wistar Rats

Dwika Audiyananda, Hendro Sudjono Yuwono, Adria Adnan

Abstract


Background: Calcium bentonite is a type of clay produced by a devitrification of volcanic ash which is often used as a traditional medicine to absorb toxins and waste products of metabolism. The aim of this study was to determine the lethal dose of calcium bentonite in Wistar rats to explore its toxicity level and safe use.

Methods: Fifty male and female Wistar rats were randomly divided into five groups for each sex. The control group was given 5 cc aquadest whereas the other four groups received calcium bentonite solution of 50 mg/kgBW, 300 mg/kgBW, 2,000 mg/kgBW, and 5,000 mg/kgBW, respectively, at a single dose. The weight was observed for up to 7 days and analyzed using the unpaired t-test and Mann-Whitney test. The death rate was calculated using the probit analysis. Median lethal dose results were then classified according to Loomis Criteria.

Results: No deaths occurred at the highest dose, suggesting that the median lethal dose value of calcium bentonite was >5,000 mg/kgBW. No weight loss occurred due to the administration of calcium bentonite and a significant increase in the body weight was even observed in the male rat group. Interestingly, a significant decrease was found in the female rats group when compared to the control group.

Conclusions: Calcium bentonite is classified as a practically non-toxic material with a median lethal dose of over 5,000 mg/kgBW.


Keywords


calcium bentonite; LD50; toxicity

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References


Bhusnure OG, Shinde MC, Vijayendra SSM, Gholve SB, Giram PS, Birajdar MJ. Phytopharmaceuticals: an emerging platform for innovation and development of new drugs from botanicals. JDDT. 2019;9(3-s):1046–57.

Gomes CF, Gomes JH, da Silva EF. Bacteriostatic and bactericidal clays: an overview. Environ Geochem Health. 2020;42(11):3507–27.

Behroozian S, Svensson SL, Li LY, Davies JE. Broad-spectrum antimicrobial and antibiofilm activity of a natural clay mineral from British Columbia, Canada. mBio. 2020;11(5):e02350–20

Moosavi M. Bentonite clay as a natural remedy: a brief review. Iran J Public Health. 2017;46(9):1176–83.

Maisanaba S, Pichardo S, Puerto M, Gutiérrez-Praena D, Cameán AM, Jos A. Toxicological evaluation of clay minerals and derived nanocomposites: A review. Environ Res. 2015;138:233–54.

Massaro M, Noto R, Riela S. Past, present and future perspectives on halloysite clay minerals. Molecules. 2020;25(20):4863.

Barile FA. Clinical toxicology: principle and mechanisms. 3rd ed. Boca Raton: CRC Press; 2019.

Loomis TA, Hayes AW. Loomis’s essential of toxicology. 5th ed. San Diego: Academic Press; 2019.

World Health Organization Regional Office for the Western Pacific. Research guidelines for evaluating the safety and efficacy of herbal medicines. Manila: WHO Regional Office for the Western Pacific; ‎1993 [cited 2021 June 6] Available from: https://apps.who.int/iris/handle/10665/207008.

Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Sasidharan S. Acute oral toxicity of methanolic seed extract of cassia fistula in mice. Molecules. 2011; 16(6):5268–82.

ThermoFisher Scientific. Safety data sheet bentonite powder. Loughborough, UK; 2020 [cited 2021 June 27] Available from: https://www.fishersci.co.uk/chemicalProductData_uk/wercs?itemCode=B/1300/60

Carl Roth GmbH+Co KG. Safe work Australia- code of practice: safety data sheet of bentonite pure. Schoemperlenstr, Karlsruhe, Germany; 2020 [cited 2021 June 27] Available from: https://carlroth.com › medias › SDB-0113-AU-EN.pdf

Maxim LD, Niebo R, McConnell EE. Bentonite toxicology and epidemiology – a review. Inha Toxicol. 2016;28(13):591–617.

Mitchell NJ , Kumi J, Aleser M, Elmore SE, Rychlik KA, Zychowski KE, et al. Short-term safety and efficacy of calcium montmorillonite clay (UPSN) in children. Am J Trop Med Hyg. 2014 ;91(4):777–85.




DOI: https://doi.org/10.15850/amj.v8n2.2227



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