Antibacterial Effect of Human Milk against Streptococcus pyogenes: an in vitro Study

Novi Vicahyani Utami, Nurul Nadiya Binti Abdul Razak, Dzulfikar D. Lukmanul Hakim

Abstract


Background: Streptococcus pyogenes infection is one of the common upper respiratory infections among children. Human milk has antibacterial properties that may play a role against infections.  The study was conducted to observe the antibacterial effect of human milk against Streptococcus pyogenes.

Methods: This was an in vitro experimental study conducted on October–November 2012 in Microbiology Laboratory, Faculty of Medicine, Universitas Padjadjaran, Bandung, using agar well diffusion method. Human milk was collected from eligible donors and tested at concentrations of 70%, 80%, 90%, and 100% dilution with water. Inhibition zone formed surrounding the wells were measured after 24 hours of incubation.

Results: All samples incubated with human mils (in concentration of 70%, 80%, 90% and 100%) inhibited the growth of Streptococcus pyogenes, with the inhibition zone mean diameters of 26.33, 28.00, 28.00 and 28.33mm, respectively, compared to the well containing sterile aquadest that did not show any zone of inhibition.

Conclusions: Human milk has an in vitro antibacterial effect against Streptococcus pyogenes. Giving human milk to babies is important for preventing infectious diseases.


Keywords


Antibacterial; human milk; in vitro; Streptococcus pyogenes

Full Text:

PDF

References


World Health Organization. The global burden of disease: 2004 update. Geneva: WHO Press; 2008.

Lucile Packard Children’s Hospital Standford. Maternal and fetal infections overview. [Online Web Page] 2010 [Cited 2012 April 26]; Available from: https://www.stanfordchildrens.org/en/topic/default?id=maternal-and-fetal-infections-overview-90-P09523&sid=.

World Health Organization. World health statistics 2011. Geneva: WHO Press; 2011.

World Health Organization. The current evidence for the burden of group A Streptococcal diseases. Geneva: WHO Press; 2005.

MedlinePlus. Bacterial infections. [Online Web Page] 2019 [Cited 2019 October 13]; Available at: https://medlineplus.gov/bacterialinfections.html.

Kalpana S, Sundar JS, Parameshwari S, Kuganantham P, Selvam JM, Valarmathi MS, et al. Isolation and identification of group A Streptococcal infection among slum children in the age group of 5–15 years in Chennai-one year prospective study. IOSR-JPBS. 2012;2(1):27–30.

Pechere JC, Kaplan EL, editors. Streptococcal pharyngitis: optimal management. Basel, Switzerland: Karger Medical and Scientific Publishers; 2004. p. 16–32.

Chantry CJ, Howard CR, Auinger P. Full breastfeeding duration and associated decrease in respiratory tract infection in US children. Pediatrics. 2006;117(2):425–32.

Goldman AS. The immune system in human milk and the developing infant. Breastfeed Med. 2007;2(4):195–204.

Lonnerdal B. Nutritional and physiologic significance of human milk proteins. Am J Clin Nutr. 2003;77(6):1537S–43S.

Aldy OS, Lubis BM, Sianturi P, Azlin E, Tjipta GD. Dampak proteksi air susu ibu terhadap infeksi. Sari Pediatri. 2009;11(3):167–73.

Chan GM, Lee ML, Rechtman DJ. Effects of a human milk-derived human milk fortifier on the antibacterial actions of human milk. Breastfeed Med. 2007;2(4):205–8.

Cid SRC, Cruz MC, Faustino V, Tuazon AO. In vitro study on the antimicrobial activity of probiotic milk against common pediatric community acquired respiratory pathogens. PIDSP Journal. 2005;9(2):25–9.

Bachrach VRG, Schwarz E, Bachrach LR. Breastfeeding and the risk of hospitalization for respiratory disease in infancy: a meta-analysis. Arch Pediatr Adolesc Med. 2003;157(3):237–43.




DOI: https://doi.org/10.15850/amj.v6n4.1697



 This Journal indexed by

                         

Creative Commons License
AMJ is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

 


View My Stats