Destructive Effect of Calcium Hypochlorite against Pseudomonas aeruginosa Biofilm

Ilma Arifani, Gita Widya Pradini, Insi Farisa Desy Arya, Adi Imam Cahyadi

Abstract


Background: Pseudomonas aeruginosa is the most common bacteria contaminating the hemodialysis water and has high capability to form a biofilm. The presence of biofilm is hazardous because it becomes a constant source of bacterial and toxin release toward the hemodialysis patient’s blood. Calcium hypochlorite (Ca(OCl)2) is an easily obtained disinfectant. This study was aimed to detect the destructive effect of Ca(OCl)2 against P. aeruginosa biofilm and the optimal disinfectant concentration required to achieve significant effect.

Methods: This experimental study was conducted in six replicates from September to October 2015 in Microbiology Laboratory of Faculty of Medicine Universitas Padjadjaran Bandung. A modified tissue culture plate method was performed to grow P. aeruginosa biofilms which were subsequently treated with Ca(OCl)2 in various chlorine concentrations, namely 20, 30, 40, and 500 parts per million (ppm). The data was analyzed using Welch Analysis of Variance (ANOVA) and Games-Howell post-hoc tests and presented in tables.

Results: Data were obtained from 36 flat-bottomed polystyrene wells. There was a statistically significant mean difference between groups [F(4, 11.92)= 91.198, p<0.001)]. All of the tested chlorine concentrations caused significant decreases in biofilm optical densities (p = 0.027 for 20 ppm and p< 0.001 for 30, 40, and 500 ppm).

Conclusions: Ca(OCl)2 with chlorine concentrations of 20, 30, 40, and 500 ppm have significant destructive effect against P. aeruginosa biofilm. The mean differences among treated groups were not significant. The most optimum concentration is 30 ppm.


Keywords


Biofilm, calcium hypochlorite, Pseudomonas aeruginosa

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References


Perkumpulan Nefrologi Indonesia. 5th report of Indonesian renal registry. Jakarta: Perkumpulan Nefrologi Indonesia; 2012.

Kwan BCH, Chow KM, Ma TKW, Cheng PMS, Leung CB, Li PKT, et al. Effect of using ultrapure dialysate for hemodialysis on the level of circulating bacterial fragment in renal failure patients. Nephron Clin Pract. 2013;123(1):246–53.

Centers for Disease Control and Prevention. Guideline for disinfection and sterilization in healthcare facilities. Atlanta: Centers for Disease Control and Prevention; 2008.

Suman E, Varghese B, Joseph N, Nisha K, Kotian MS. The bacterial biofilms in dialysis water systems and the effect of the sub inhibitory concentrations of chlorine on them. J Clin Diagn Res. 2013;7(5):849–52.

Montanari LB, Sartori FG, Cardoso MJ, Varo SD, Pires RH, Leite CQ, et al. Microbiological contamination of a hemodialysis center water distribution system. Rev Inst Med Trop Sao Paulo. 2009;51(1):37–43.

Oumokhtar B, Lalami AEO, Mahmoud M, Berrada S, Arrayhani M, Houssaini TS. Prevent infection linked to the dialysis water in a hemodialysis center in Fez city (Morocco). Pan Afr Med J. 2013;16(1):122–6.

Arvanitidou M, Vayona A, Spanakis N, Tsakris A. Occurrence and antimicrobial resistance of Gram-negative bacteria isolated in haemodialysis water and dialysate of renal units: results of a Greek multicentre study. J Appl Microbiol. 2003;95(1):180–5.

Ciobotaro P, Fialko A, Nadir E, Oved M, Bardenstein R, Gershkoviz P, et al. P205: an outbreak of polyclonal pseudomonas aeruginosa bacteremia in hemodialysis patients. Antimicrob Resist Infect Control. 2013;2(Suppl 1):205.

Al-Jailawi MH, Ameen RS, Al-Jeboori MR. Effect of Disinfectants on Antibiotics Susceptibility of Pseudomonas aeruginosa. J Appl Biotechnol. 2013;1(1):54–63.

Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis. 2011;15(4):305–11.

World Health Organization. Fact sheet 2.19: calcium hypochlorite. [cited 2015 December 1]. Available from: http://www.who.int/water_sanitation_health/hygiene/emergencies/fs2_19.pdf.

Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol. 2006;24(1):25–9.

Dutta A, Saunders WP. Comparative evaluation of calcium hypochlorite and sodium hypochlorite on soft-tissue dissolution. J Endod. 2012;38(10):1395–8.

Wang L, Bassiri M, Najafi R, Najafi K, Yang J, Khosrovi B, et al. Stabilized hypochlorous acid: a component of the inorganic armamentarium of innate immunity. J Burns Wounds. 2007;6(1):65-79.

Tote´ K, Horemans T, Berghe DV, Maes L, Cos P. Inhibitory effect of biocides on the viable masses and matrices of Staphylococcus aureus and Pseudomonas aeruginosa biofilms. Appl Environ Microbiol. 2010;76(10):3135–42.

Fukuzaki S. Mechanisms of actions of sodium hypochlorite in cleaning and disinfection processes. Biocontrol Sci. 2006;11(4):147–57.

Small DA, Chang W, Toghrol F, Bentley WE. Toxicogenomic analysis of sodium hypochlorite antimicrobial mechanisms in Pseudomonas aeruginosa. Appl Microbiol Biotechnol. 2007;74(1):176–85.

Behnke S, Parker AE, Woodall D, Camper AK. Comparing the chlorine disinfection of detached biofilm clusters with those of sessile biofilms and planktonic cells in single and dual-species cultures. Appl Environ Microbiol. 2011;77(20):7176–84.

Borges CR, Lascowski KM, Filho NR, Pelayo JS. Microbiological quality of water and dialysate in a haemodialysis unit in Ponta Grossa-PR, Brazil. J Appl Microbiol. 2007;103(5):1791–7.

Agar JW, Perkins A, Heaf JG. Home hemodialysis: infrastructure, water, and machines in the home. Hemodial Int. 2015;19(Suppl 1):S93–111.




DOI: http://dx.doi.org/10.15850/amj.v4n3.1205

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