Perbandingan Aktivitas Antioksidan dan Toksisitas Antara Drop Vitamin A dari Karotenoid Kulit Pisang Ambon dan ß-Karoten

Harka Prasetya, Israhnanto Isradji, Suparmi Suparmi, Adrian Hardec, Muhammad Fahryzal, Laili Durotul Azizah, Dita Ferwina Utari Ashar

Abstract


Pemanfaatan kulit pisang akibat peningkatan konsumsi pisang ambon perlu dilakukan, salah satunya sebagai alternatif sumber vitamin A alami (SUVITAL) untuk mengatasi kasus kekurangan vitamin A (KVA). Potensi karotenoid kulit pisang ambon sebagai SUVITAL belum banyak diteliti. Penelitian ini bertujuan menguji aktivitas antioksidan dan toksisitas terhadap hati dan ginjal dari drop vitamin A karotenoid kulit pisang ambon (DKKP) dibanding dengan β karoten (DBKM) pada kelinci (Oryctolagus cuniculus) galur New Zealand White. DKKP dan DBKM dibuat sesuai formula vitamin A per 50.000 IU/mL. Penelitian ini dilakukan di Laboratorium Biologi Fakultas Kedokteran Universitas Islam Sultan Agung selama September–Oktober 2015. Sebanyak 12 ekor kelinci jantan dibagi menjadi 3 kelompok: kontrol, DKKP, dan DBKM. Drop diberikan per oral sebanyak 5 mL/ekor/hari selama 7 hari. Efek antioksidan diuji berdasar atas kadar malondialdehyde (MDA) dan retinol serum darah, sedangkan toksisitas terhadap hati dan ginjal ditentukan berdasar derajat kerusakan hepatosit dan sel epitel tubulus proksimal ginjal. Hasil uji LSD menunjukkan bahwa kadar MDA dan retinol serum darah kelinci yang diberi DKKP tidak berbeda signifikan (p>0,05) dibanding dengan DBKM, demikian juga dengan skor kerusakan hepatosit dan sel epitel tubulus proksimal ginjal. Aktivitas antioksidan drop vitamin A dari karotenoid kulit pisang ambon sama dengan drop β-karoten murni. Drop vitamin A dari karotenoid kulit pisang tidak toksik terhadap histopatologi hati dan tubulus proksimal ginjal. [MKB. 2016;49(1):1–7]
 
Kata kunci: Antioksidan, drop vitamin A, karotenoid, kulit pisang ambon, toksisitas


Antioxidant Activity and Toxicity of Vitamin A Drop from Ambon Peel Carotenoid and Pure ß-carotene
 
The increased consumption of ambon banana has resulted in increased amount of banana peel; which opens an opportunity to use them as a natural vitamin A alternative source to prevent vitamin A deficiency (VAD). The potential use of banana peel as a vitamin A supplement has not been much investigated. This study was conducted to measure the antioxidant activities and toxicity of vitamin A drop from carotenoids of Ambon banana peel (DKKP) in comparison with the vitamin A drop containing pure β-carotene (DBKM) on New Zealand White rabbits’ liver and kidney (Oryctolagus cuniculus) . The DKKP and DBKM were formulated according to the formula of vitamin A per 50,000 IU/ml. Twelve male rabbits were divided into 3 groups, i.e., control, DKKP, and DBKM. DKKP and DKKP groups received 5 ml/day oral dose for 7 days. Malondialdehyde (MDA) and retinol from blood serum were measured as the parameters of antioxidant activities while the hepatocyte and proximal tubule epithelium damage score were used to detect toxicity in liver and kidney. LSD test results showed that the levels of MDA and retinol in the blood serum of the DKKP group were not significantly different (p> 0.05) from those of the DBKM group. A similar situation was also seen for the hepatocyte damage score of the liver and proximal tubular epithelial cells of kidney. The antioxidant activities of vitamin A drop from banana peel carotenoid was equal to that of the vitamin A drop from pure β-carotene. Vitamin A drop from carotenoid of banana peel is safe to be consumed and have no effect on hepatic histopathology and renal proximal tubules. [MKB. 2016;49(1):1–7]
 
Key words: Ambon banana peel, antioxidant, carotenoid, toxicity, vitamin A drop

 

DOI: 10.15395/mkb.v49n1.981


Keywords


Antioksidan, drop vitamin A, karotenoid, kulit pisang ambon, toksisitas 

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References


Kurhade AH, Waghmare JS, Effect of banana peel oleoresin on oxidative stability of sunflower and soybean oil. J Food Processing Preservation. 2015;39(6):1788–97.

Suparmi, Harka P. Isolation and identification of carotenoid pigments of yellow ambon banana peel (Musa paradisiaca sapientum L.). Proceeding of Humboldt Kolleg: Synergy, Networking and The Role of Fundamental Research Development in ASEAN in conjunction with the International Conference on Natural Sciences (ICONS); 2011 July 9–11; Malang. Indonesia; Ma Chung University: 2011.

Suparmi, Harka P. Aktifitas antioksidan ekstrak kasar pigmen karotenoid pada kulit pisang ambon kuning (Musa parasidiaca sapientum): potensi sebagai suplemen vitamin A. Sains Medika. 2012;4(1):78–88.

Danielle N, Smarsh, Carey A, Williams, Oxidative stress and antioxidant status in standardbreds: Effect of age and training in resting plasma and muscle. J Equine Veterinary Sci. 2017. 48:93–102,

Diding HP, Endang LS, A Guntur H. Ekstrak etanol propolis isolat menurunkan derajat inflamasi dan kadar malondialdehid pada serum tikus model sepsis. MKB. 2013; 45(3):161–6.

Putri V, Armaya A, Herri SS. Perlindungan hepatotoksisitas ekstrak metanol pegagan dibanding vitamin E pada tikus model hepatitis. MKB. 2014;42(3):101–7.

Asep S, Herri SS. Antioksidan ekstrak air biji kopi robusta Lampung dalam menghambat degenerasi sel hati tikus model hepatitis yang diinduksi CCL4. MKB. 2012;44(3):127–32.

Permiasih D. Penilaian status vitamin A secara biokimia. Gizi Indon. 2008;31(2):92–7.

Stice CP, Wang XD. Carotenoids and alcoholic liver disease. Hepatobil Surg Nutr. 2013; 2(5):244–7.

Aquino JDS, Vasconcelos MHDA, Pessoa DCNDP, Soares JKB, Prado JPDS, Mascarenhas RDJ, dkk. Intake of cookies made with buriti oil (Mauritia flexuosa) improves vitamin A status and lipid profiles in young rats. Food Function. 2016;7(10):4442–50.

Suparmi, Harka P, Martanto M, Lasmono TS. Effects of β-carotene from yellow ambon banana peel on rat serum retinol level. J Pure App Chem Res. 2014;3(2):83–8.

Mahassni S, N Al-Shaikh. Effects of vitamin A overdose on rat’s organs involved in immunity and vitamin A storage. Acta Alimentaria. 2014.43(3):452–8.

Hosseini F, Naseri MK, Badavi M, Ghaffari MA, Shahbazian H, Rashidi I. Effect of beta carotene on lipid peroxidation and antioxidant status following renal ischemia/reperfusion injury in rat. Scand J Clin Lab Invest. 2010;70(4):259–63.

Bühler V. Generic drug formulations. Swiss: BASF Fine Chemicals., [diunduh 20 November 2014]. Tersedia dari: diambil dari: www.ut.ee/ARFA/tehnol/generic.pdf.

Panjaitan TD, Budhi P, Limantara L. Peranan karotenoid alami dalam menangkal radikal bebas dalam tubuh [tesis]. Medan: Universitas Sumatera; 2010.

Orak HH, KaramaÄM, Orak A, Amarowicz R, Antioxidant potential and phenolic compounds of some widely consumed Turkish white bean (Phaseolus vulgaris L.) varieties. Polish J Food Nutr Sci. 2016; 66(4):253–60.

Ramanathan VS, Hensley G, French S, Eysselein V, Chung D, Reicher S and Pham B. Hypervitaminosis A inducing intra-hepatic cholestasis – a rare case report. Exp Mol Pathol. 2009;88;324–5.

El-baky MSA, Ahmed LF. Effect of some fruits and vegetables peels extract on CCl4 induced hepatic injury in rats. Med J Cairo Univ. 2011;79(1):49–57.


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