Nuclei DNA Damage Due to Extreme High-Temperature Exposure during Forensic Identification Examination

Ahmad Yudianto, Masniari Novita, Ariyanto Wibowo, Fery Setiawan

Abstract


Accurate personal identification is important in investigations because an error in the identification process may bring fatal consequences during trial. The most common identification process is the Deoxyribonucleic acid [DNA] analysis. Degraded DNA sample due to extremely high-temperature exposure may limit DNA analysis. This study aimed to analyze DNA damage patterns caused by an extremely high temperature using STR (short tandem repeat) CODIS marker. This study was conducted at the Forensic and Medicolegal Department, Laboratorium Balai Besar Kesehatan Surabaya, Ministry of Health of the Republic of Indonesia, Human Genetic Study Group of Universitas Airlangga, and Faculty of Science and Technology of Universitas Brawijaya Malang from July until October 2009. Results of PCR visualization using STR CODIS for costae demonstrated that the THO1 detection (+) in 1,2500C - 40’: 25% and the TPOX detection (+) in 1,0000C - 30’: 50% whereas the results from molar teeth showed that the THOI locus detection (+) in 1,2500C - 30’: 25% and TPOX in 1,0000C - 40’: 50%. Results for PCR visualization using mini-STR CODIS for the costae presented that the mini-THOI in 1,2500C - 20’: 50% (+) while for the molar tooth the mini-THOI in 1,2500C - 30’ : 25% (+) and mini-TPOX in 1,0000C - 40’ : 50% (+). All loci were detected on costae and second molar teeth samples of the control group. Thus, extreme high-temperature exposure significantly decreased the DNA level of second costae and second molar tooth. Sequence patterns of STR loci successfully detected were TPOX, THO1, and CSF1PO.


Kerusakan DNA Inti Karena Paparan Suhu Tinggi Selama Proses Identifikasi Forensik

Identifikasi melalui DNA secara tepat penting dalam penyelidikan karena kesalahan akan berakibat fatal selama proses persidangan. Salah satu keterbatasan adalah DNA yang terdegradasi karena paparan suhu yang sangat tinggi. Penelitian ini bertujuan menganalisis pola kerusakan DNA akibat suhu sangat tinggi menggunakan penanda CODIS STR (short tandem repeat). Penelitian dilakukan di Instalasi Kedokteran Forensik, Laboratorium Kemenkes, Human Genetic Study Group, dan Universitas Brawijaya Malang pada periode Juli sampai Oktober 2009. Visualisasi PCR menggunakan STR CODIS untuk costae adalah sebagai berikut: deteksi THO1 (+) pada 12500C - 40’: 25%, deteksi TPOX (+) pada 10000C - 30’: 50%, sedangkan hasil dari gigi molar adalah sebagai berikut: THOI locus detection (+) di 12500C - 30’: 25% dan TPOX di 10000C - 40’: 50%. Hasil visualisasi PCR menggunakan mini-STR CODIS untuk costae adalah sebagai berikut: mini-THOI pada 12500C - 20’: 50% (+) dan untuk gigi molar: mini-THOI pada 12500C-30': 25% (+ ) dan mini-TPOX di 10000C - 40’ : 50%  (+). Semua lokus terdeteksi pada kelompok kontrol pada sampel costae dan gigi molar kedua. Kesimpulannya, paparan suhu tinggi yang ekstrim secara signifikan menurunkan tingkat DNA kosta dan gigi molar kedua.

 


Keywords


Costae, DNA level, Extreme high temperature, molar tooth, Short Tandem Repeat Combined DNA Index System

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References


Yudianto A. Analisis DNA tulang dan gigi pada lokus short tandem repeats-combined DNA index system (STR-CODIS), Y-Chromosome STRs & mitochondrial DNA (mtDNA) akibat efek paparan panas suhu tinggi [dissertation] Surabaya: Universitas Airlangga; 2010. Available from: http://repository.unair.ac.id/32039/.

Yudianto A. Pemeriksaan identifikasi forensik molekuler. Surabaya: Global Persada Press; 2015.

Yudianto A. Pemeriksaan forensik DNA tulang dan gigi : identifikasi pada DNA Lokus STR CODIS, Y-STRs dan mtDNA. Surabaya: Sintesa; 2020.

Butler JM. The future of forensic DNA analysis. Philos Trans R Soc Lond B Biol Sci. 2015;370(1674):20140252.

Karni M, Zidon D, Polak P, Zalevsky Z, Shefi O. Thermal degradation of DNA. DNA Cell Biol. 2013;32(6):298–301.

Sultana GNN, Sultan MZ. Mitochondrial DNA and methods for forensic identification. J Forensic Sci & Criminal Inves. 2018;9(1):1–6.

Kantidze OI, Velichko AK, Luzhin AV, Razin SV. Heat stress-induced DNA damage. Acta Naturae. 2016;8(2):75–8.

Krishan K, Kanchan T, Garg AK. Dental evidence in forensic identification-an overview, methodology and present status. Open Dent J. 2015;9:250–6.

Ibrahim AN, Bhat V, Shenoy SM, Shetty VA. Quantitative evaluation of DNA from the tooth pulp exposed to varying temperatures. NUJHS. 2016;6(3):6–9.

Tozzo P, Scrivano S, Sanavio M, Caenazzo L. The Role of DNA degradation in the estimation of post-mortem interval: a systematic review of the current literature. Int J Mol Sci. 2020;21(10):3540.

Latham KE, Miller JJ. DNA recovery and analysis from skeletal material in modern forensic contexts. Forensic Sci Res. 2018; 4(1):51–9.

Giglia-Mari G, Zotter A, Vermeulen W. DNA damage response. Cold Spring Harb Perspect Biol. 2011;3(1):a000745.




DOI: https://doi.org/10.15395/mkb.v52n4.2143

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