Studi Gambaran Histopatologi Hepar Tikus Putih Strain Wistar yang Diinduksi Aspirin Pascapemberian Ekstrak Etanol Umbi Iles-iles (Amorphophallus variabilis Bl.) Selama 7 Hari

Arfatul Makiyah, Lela Laelatul Khumaisah

Abstract


Paradigma yang berkembang di masyarakat kita saat ini ramuan tradisional dikarenakan bahan-bahannya berasal dari alam adalah tidak berbahaya dan tidak mempunyai efek samping. Tujuan penelitian adalah mengetahui gambaran histopatologi hepar tikus putih strain Wistar yang diinduksi aspirin pasca pemberian ekstrak umbi iles-iles (Amorphophallus variabilis Bl.). Penelitian ini dilaksanakan di Laboratorium Farmakologi Universitas Padjadjaran pada bulan April–Mei 2018.  Tikus putih strain Wistar sebanyak 20 ekor dibagi menjadi 4 kelompok. Tikus putih strain wistar pada setiap perlakuan diberikan ekstrak iles-iles secara oral selama 7 hari pada tanggal 8–14 April 2018. Parameter gambaran histopatologi hepar tikus putih strain wistar yang diinduksi aspirin pascapemberian ekstrak umbi iles-iles (Amorphophallus variabilis Bl.) dengan dosis yang berbeda (0 mg/kgBB, 220 mg/kgBB, 110 mg/kgBB, 55 mg/kgBB) adalah preparat histopatologi hepar masing-masing dilakukan 5 lapang pandang mikroskopik. Hasil penelitian hasil Uji Kruskall Wallis menunjukkan bahwa kelompok tikus putih strain Wistar yang diberi ekstrak dengan dosis masing-masing 110 dan 220 mg/kgBB lebih banyak (p<0,05) tikus putih strain Wistar yang mengalami penebalan septa interalveolaris dibanding dengan kelompok I (kontrol). Pemberian ekstrak terstandar etanol umbi iles-iles dosis tunggal 110 mg/kgBB dan 220 mg/kgBB pada tikus putih strain Wistar tampak menimbulkan perubahan histopatologi berupa radang, nekrosis, kongesti, dan perlemakan hepar.

 Kata kunci:  Ekstrak Etanol, histopatologi, umbi Iles-iles (Amorphophallus variabilis Bl.)

 

    Histopathology Overview of Hepar in White Wistar Rats Induced with Aspirin After 7 Days Administration of Ethanol Extracts of Iles-iles Bulb (Amorphophallus variabilis Bl.)

The paradigm that develops in our society today is traditional ingredients because the ingredients are from nature is harmless and has no side effects. The aim of this study was to find out the histopathologic features of wistar-induced aspirin-induced Wistar rats after administration of iles-iles bulb extract (Amorphophallus variabilis Bl.). This research was conducted at Pharmacology Laboratory of Padjadjaran University in April-May 2018. Twenty Wistar white rat strain were divided into 4 groups consisted of 4 doses of iles-iles extract administration i.e., 0, 55, 110, and 220 mg/kg BW. Wistar strain white rats on each treatment were administered iles-iles extract orally for 7 days on days 8-14 of April 2018. Histopathologic image parameters of Wistar rats induced with aspirin after 7 days administrations of iles-iles extract (Amorphophallus variabilis Bl.) were observed. The results showed that the results of Kruskall Wallis test showed that the group of white rat strains Wistar extracted with dose respectively 110 and 220 mg / kgBW more (p <0.05) white rat strain Wistar thickened sepal interalveolaris compared to group I (control). Provision of standardized ethanol extract of iles-iles tuber single dose of 110 mg / kgBW and 220 mg / kgBW in white mice of Wistar strains appears to cause histopathological changes such as inflammation, necrosis, congestion and hepatic fatty acids. 

Key words: Ethanol extract, histopathology, iles-iles tubers (Amorphophallus  variabilis Bl.),

 


Keywords


Ekstrak Etanol, histopatologi, umbi Iles-iles (Amorphophallus variabilis Bl.)

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