Peran Induksi TNF-α Serial Doses dalam Peningkatan VEGF dan PDGF Mesenchymal Stem Cells

Agung Putra, Ananta Hutagalung, Ihdina Hanifa Hasanal, Setyo Trisnadi, Durrotul Djannah, Erwin Budi Cahyono, Yulice Soraya Nur Intan

Abstract


Mesenchymal stem cell (MSC) mempunyai kemampuan immunoregulasi dan regenerasi melalui supresi pelepasan mediator proinflamasidan peningkatan molekul proliferasi terutama vascular endothelial growth factor (VEGF) dan platelet-derived growth factor (PDGF). Mesenchymal stem cell yang diaktivasi TNF-α dengan dosis tertentu mampu meningkatkan sekresi VEGF dan PDGF, namun dosis optimal TNF-α yang mampu memaksimalkan ekspresi molekul tersebut belum diketahui secara pasti. Variasi dosis TNF-α digunakan pada penelitian ini dengan tujuan mengetahui dosis optimal, rendah, dan tinggi TNF-α dalam memaksimalkan ekspresi VEGF dan PDGF. Penelitian ini mengunakan post-test only control group design dengan 5 kelompok penelitian, terdiri atas satu kelompok kontrol (K) dan 4 kelompok perlakuan (P) (TNF-α= 5, 10, 40, 80 ng/mL) yang diinduksikan pada MSC dengan inkubasi 24 jam, kemudian kadar PDGF dan VEGF diukur dengan metode ELISA. Penelitian ini dilakukan antara bulan September–November 2017 di Laboratorium Stem Cell and Cancer Research (SCCR), Fakultas Kedokteran, Universitas Islam Sultan Agung, Semarang. Hasil penelitian menunjukkan peningkatan kadar PDGF dan VEGF secara signifikan (p<0,05) dimulai dari dosis TNF-α 5 ng/mL, optimal padadosis 10 ng/mL dan mulai terjadi penurunan pada dosis 40 ng/mL. Induksi TNF-α pada MSC mampu memaksimalkan kadar VEGF dan PDGF pada dosis 10 ng/mL.

Kata kunci: MSC, PDGF, TNF-α, VEGF

 

The Effect of TNF-α Serial Doses Induced MSC on Increasing VEGF dan PDGF

Mesenchymal Stem Cells (MSCs) have immunoregulation and regeneration capability by supressing the release of proinflammatory mediator and increasing proliferative molecules, particulary vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) TNF-α activated MSC with certain dose have ability to increase VEGF and PDGF level, however the exact optimum dose of TNF-α to optimize the level of VEGF and PDGF is unclear. In this study, we used TNF-α dose variation to determine the optimum, low and high dose of TNF-α in optimizing VEGF and PDGF expression. This study used to post-test only control group design.There were five study groups, consist of one control (K) and four treatment groups(P) were treated with TNF-α5, 10, 40 and 80 ng/mL for 24 hours, all groupswere measured PDGF and VEGF levels using ELISA. This research was conducted between September-November 2017 at the Stem Cell and Cancer Research Laboratory (SCCR), Faculty of Medicine, Sultan Agung Islamic University, Semarang. The results show significantly increased of PDGF and VEGF levels (p<0.05) from TNF-α 5 ng/mL as initiation dose, 10 ng/mL as optimum dose and the decrease dose start at 40 ng/mL. TNF-induced MSCs have ability to increase VEGF and PDGF level with optimum dose at 10 ng/mL.

Key words: MSC, PDGF, TNF-α, VEGF


Keywords


MSC, TNF-α, VEGF, PDGF

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