Sapodilla (Manilkara zapota) Broth as an Alternative Media for Candida albicans

Chen Chui Ying, Ine Kuswardinah, Elsa Pudji Setiawati

Abstract


Objective: To determine whether sapodilla can be used to grow Candida albicans. Among all the high galactose and arabinose content fruits, the sapodilla was chosen because it is available year round and can get easily in market. Other than that, it also contains vitamins, calcium and phosphorus which are very useful for fungi growth.

Methods: This study used an experimental study as a method of research. The researcher culture Candida albicans on the experimental sapodilla media and identifies the morphology of the fungi by using Gram staining method. The experiment will be replicated two times to get accurate result. The procedure of this experiment constitute of sapodilla media preparation, sapodilla media observation, organism preparation, planting and incubation, observation of fungal colonies and identification of the fungi.

Results: In 0%, there was no fungal growth at all. In 5%, there was mild density of fungal colonies. In 10%, there was moderate density of fungal colonies and in 15% the fungal grew with very dense colonies.

Conclusions: Sapodilla (Manilkara zapota) broth can be used as an alternative media for Candida albicans.

Keywords: Alternative media, Candida albicans, Sapodilla broth


DOI: 10.15850/ijihs.v5n1.965


Full Text:

PDF

References


Chanda SV, Nagani KV. Antioxidant capacity of Manilkara zapota L. leaves extracts evaluated by four in vitro methods. Nat Sci. 2010;8(10):260‒6.

Annous BA, Fratamico PM, Smith JL. Quorum sensing in biofilms: why bacteria behave the way they do. J Food Sci. 2009;74(1):24‒37.

Janelle MH. Sabouraud agar for fungal growth. In: Gupta VK, Tuohy MG, Ayyachamy M, Turner KM, O’Donovan A, editors. Laboratory protocols in fungal biology. 2nd ed. New York: Springer; 2013. p. 211–6.

Shi J, Chinn MS, Sharma-Shivappa RR. Interactions between fungal growth, substrate utilization, and enzyme production during solid substrate cultivation of Phanerochaete chrysosporium on cotton stalks. Bioproc Biosyst Eng. 2014;37(12):2463‒73.

Ahn M, Yoon KC, Ryu SK, Cho NC, You IC. Clinical aspects and prognosis of mixed microbial (bacterial and fungal) keratitis. Cornea. 2011;30(4):409‒13

Mason KL, Downward JRE, Mason KD, Falkowski NR, Eaton KA, Kao JY, et al. Candida albicans and bacterial microbiota interactions in the cecum during recolonization following broad-spectrum antibiotic therapy. Infect Immunol. 2012;80(10):3371‒80.

Pandiyan P, Conti HR, Zheng L, Peterson AC, Mathern DR, Hernández-Santos N, et al. CD4+ CD25+ Foxp3+ regulatory T cells promote Th17 cells in vitro and enhance host resistance in mouse Candida albicans Th17 cell infection model. Immunity. 2011;34(3);422–34.

Woo PF, Yim HS, Khoo HE, Sia CM, Ang YK. Effects of extraction conditions on antioxidant properties of sapodilla fruit (Manilkara zapota). Food Res Int. 2013;20(5);2065–72.

Patel CJ, Asija S, Patel P, Dhruv M, Tyagi S. Medicated chewing gum: a modern era of novel drug delivery system. RJPDFT. 2012;4(6):293‒9.

Willey J, Sherwood L, Woolverton C. Prescott’s microbiology. 9th ed. New York: McGraw-Hill Companies Inc; 2014.

Misra VC, Randhawa HS. Occurrence and significance of Cryptococcus neoformans in vegetables and fruits. Indian J Chest Dis Allied Sci. 2000;16;42(4):317–22.

Deivanayaki M, Iruthayaraj A. Alternative vegetable nutrient source for microbial growth. Int J Bio Sci. 2012;2(5):47–51.


Refbacks

  • There are currently no refbacks.




This journal indexed by:

             

  

Creative Commons License
IJIHS is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License



View My Stats