Sapodilla (Manilkara Zapota) Broth as an Alternative Media for Candida Albicans

Chen Chui Ying, Ine Kuswardinah, Elsa Pudji Setiawati

Abstract


Objective: Sabouraud dextrose agar is used to support growth of fungi due to its’ high glucose content. Sabouraud dextrose agar is expensive because it’s imported from United States. Hence, the researcher wants to offer an alternative media to support growth of fungi. Among all the high galactose and arabinose content fruits, the researcher chose sapodilla because it is available year round and can get easily in market. Other than that, it also contains vitamins, calcium and phosphorus which are very useful for fungi growth. The aim of this research is to determine whether sapodilla can be used to grow Candida albicans.

Methods: This research uses an experimental study as a method of research. The researcher culture Candida albicans on the experimental sapodilla media and identifies the morphology of the fungi by using Gram staining method. The experiment will be replicated two times to get accurate result. The procedure of this experiment constitute of sapodilla media preparation, sapodilla media observation, organism preparation, planting and incubation, observation of fungal colonies and identification of the fungi.

Results: In 0%, there is no fungal growth at all. In 5%, there is mild density of fungal colonies. In 10%, there is moderate density of fungal colonies and in 15% the fungal grew with very dense colonies.

Conclusion: Sapodilla (Manilkara Zapota) broth can be used as an alternative media for Candida albicans.

Keywords: Sapodilla broth, Alternative media, Candida albicans


DOI: 10.15850/ijihs.v5n1.


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