Ethanol Extract of Stylissa carteri Induces Cell Death in Parental and Paclitaxel-Resistant Cervical Cancer Cells

Isna Nisrina Hardani, Fachreza Aryo Damara, Annisa Dewi Nugrahani, Muhammad Hasan Bashari

Abstract


Objective: To evaluate the cytotoxic effects of ethanol extract from marine sponge Stylissa carteri in both parental and paclitaxel-resistant HeLa cervical cancer cells.

Methods: This was an experimental in-vitro study subjected ethanol extract from Stylissa carteri obtained from Pramuka Island, Kepulauan Seribu National Park, Jakarta. Both parental and paclitaxel-resistant HeLa cells were treated with ethanol extract followed by microscopic observation and MTT assay. The IC50 and drug curves were analyzed using four-parametric logistic regression by SigmaPlot for Windows version 12.0 from Systat Software Inc., USA.

Results: Ethanol extract from Stylissa carteri triggered cell death-associated morphological changes in parental Hela cells. It demonstrated cytotoxic activities with an IC50 value of 1 ppm. Importantly, this extract also triggered cell death in paclitaxel-resistant HeLa cells. The IC50 of ethanol extract of Stylissa carteri was 4 ppm in paclitaxel-resistant HeLa cells.

Conclusions: There is a potential cytotoxic activity of Stylissa carteri that is not only confirmed in parental HeLa cells but also in paclitaxel-resistant HeLa cells.

Keywords: Cervical cancer, paclitaxel resistance, HeLa cells, cytotoxicity
assay, Stylissa carteri

 

DOI: 10.15850/ijihs.v6n2.1378


Keywords


Cervical cancer; paclitaxel resistance; HeLa cells; cytotoxicity assay; Stylissa carteri

Full Text:

PDF

References


Stewart BW, Wild CP. World cancer report 2014. Geneva: World Health Organization; 2014.

Wahidin M, Noviani R, Hermawan S, Andriani V, Ardian A, Djarir H. Population-based cancer registration in Indonesia. Asian Pacific J Cancer Prev. 2012;13(4):1709–10.

Marth C, Landoni F, Mahner S, McCormack M, Gonzalez-Martin A, Colombo N, et al. Cervical cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2017;28(Suppl 4):iv72–83.

Zhu H, Luo H, Zhang W, Shen Z, Hu X, Zhu X. Molecular mechanisms of cisplatin resistance in cervical cancer. Drug Des Devel Ther [serial on the internet]. 2016 Jun [cited 2018 Apr 9];2016(10):[about 11p.]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907638/.

Peng X, Gong F, Chen Y, Jiang Y, Liu J, Yu M, et al. Autophagy promotes paclitaxel resistance of cervical cancer cells: Involvement of Warburg effect activated hypoxia-induced factor 1-α-mediated signaling. Cell Death Dis. [serial on the internet]. 2014 Aug [cited 2018 Apr 7];5(8):[about 11p.]. Available from: https://www.nature.com/articles/cddis2014297.

O’Rourke A, Kremb S, Bader TM, Helfer M, Schmitt-Kopplin P, Gerwick WH, et al. Alkaloids from the sponge Stylissa carteri present prospective scaffolds for the inhibition of human immunodeficiency virus 1 (HIV-1). Mar Drugs. [serial on the internet]. 2016 Feb [cited 2018 Apr 9];102(6):[about 10p.]. Available from: http://www.mdpi.com/1660-3397/14/2/28.

McDermott M, Eustace AJ, Busschots S, Breen L, Crown J, Clynes M, et al. In vitro development of chemotherapy and targeted therapy drug-resistant cancer cell lines: a practical guide with case studies. Front Oncol [serial on the internet]. 2014 Mar [cited 2018 Apr 9];4(40):[about 16p.]. Available from: 2https://www.frontiersin.org/articles/10.3389/fonc.2014.00040/full.

Bashari MH, Fan F, Vallet S, Sattler M, Arn M, Luckner-Minden C, et al. Mcl-1 confers protection of Her2-positive breast cancer cells to hypoxia: therapeutic implications. Breast Cancer Res [serial on the internet]. 2016 Mar [cited 2018 Apr 9];18(1):[about 15p.]. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26921175%5Cnhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4769490.

Dewi AS, Suhita A. When East meets West: the chemistry of Indonesian sponges and Australian nudibranc




DOI: http://dx.doi.org/10.15850/1378

Refbacks

  • There are currently no refbacks.




This journal indexed by:

            

  

Creative Commons License
IJIHS is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License



View My Stats