Ethanol Extract of Stylissa carteri Induces Cell Death in Parental and Paclitaxel-Resistant Cervical Cancer Cells

Isna Nisrina Hardani, Fachreza Aryo Damara, Annisa Dewi Nugrahani, Muhammad Hasan Bashari


Objective: To evaluate the cytotoxic effects of ethanol extract of marine sponge Stylissa carteri in both parental and paclitaxel-resistant HeLa cervical cancer cells.

Methods: This was experimental in-vitro study subjected ethanol extract Stylissa carteri obtained from Pramuka Island, Kepulauan Seribu National Park, Jakarta. Paclitaxel-resistant HeLa cells were treated with ethanol extract followed by microscopic observation and MTT assay. The IC50 and drug curves were analyzed using four-parametric logistic regression by SigmaPlot for Windows version 12.0.

Results: Our data showed that ethanol extract of Stylissa carteri triggered cell death-associated morphological changes as well as inhibited cell viability. The IC50 was 1 ppm. Importantly, this ethanol extract of Stylissa carteri was also triggered cell death in paclitaxel-resistant HeLa cells. The IC50 was 4 ppm.  

Conclusion: This study revealed that a potential cytotoxic activity of Stylissa carteri not only in parental HeLa cells but also in paclitaxel-resistant HeLa cells. This recommends for further study to discover a novel compound contained in marine sponge.


Cervical cancer; paclitaxel resistance; HeLa cells; cytotoxicity assay; Stylissa carteri


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